Method for quickly evaluating influence degree of organic acid level for alcohol metabolism of Maotai-flavour liquor
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A Maotai-flavor liquor, a technology of influence degree, applied in biochemical equipment and methods, microbial determination/inspection, color/spectral characteristic measurement, etc. Effective evaluation of the effect
Pending Publication Date: 2021-04-02
贵州国台酒业集团股份有限公司 +1
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At present, although there are some published research methods that can detect and evaluate the degree of alcohol metabolism of different liquors, these methods mainly rely on human experiments and
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Embodiment 1
[0023] Embodiment 1, step one: carry out the preparation of alcohol dehydrogenase (ADH) enzymatic activity assay substrate mixture in 96-well microplate orifice plate, divide 96-well microplate plate into 4 groups, each group is 11 wells 150 μL of 100 mmol / L glycine-NaOH buffer solution (pH 10.0), 20 μL of 24 mmol / L NAD+ (nicotinamide adenine dinucleotide, coenzyme I for short), 20 μL of liquor sample .
[0024] According to the difference of each group, the liquor samples of each group are: 20uL of a Maotai-flavor liquor sample 1# (the initial alcohol content is 53% alc / vol, the final concentration is 100mM), 20μL of a Maotai-flavor liquor Liquor sample 2# (initial alcohol content is 53%alc / vol, final concentration is 100mM), 20μL of a sauce-flavored liquor sample 3# (initial alcohol content is 53%alc / vol, final concentration is 100mM), 53% alc / vol edible alcohol standard solution. Add 5 μL of lactic acid with concentrations of 0ppm, 200ppm, 400ppm, 800ppm, 1200ppm, 1600ppm...
Embodiment 2
[0032] Step 1: Prepare the substrate mixture for the determination of alcohol dehydrogenase (ADH) enzyme activity in a 96-well enzyme-labeled well plate, and add: 150 μL of 100 mmol / L glycine-NaOH buffer solution (pH10 .0), 20 μL of 24mmol / LNAD+, 20 μL of a Maotai-flavored liquor sample (the initial alcohol content of the wine sample is 53% alc / vol, the final concentration is 100mM, 5uL of organic acid was added to each of the 12 wells, according to the The types of organic acids are divided into three groups. The types of organic acids are lactic acid, acetic acid, and caproic acid. The 4 wells of one group are respectively added with lactic acid at a concentration of 0ppm, 500ppm, 1000ppm, and 1500ppm, and the 4 wells of the other group are respectively added with a concentration of 0ppm, 500ppm, 1000ppm, 1500ppm of acetic acid, the 4 wells of the last group were added with the concentration of 0ppm, 500ppm, 1000ppm, 1500ppm of hexanoic acid; then use ddH 2 O (double distill...
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Abstract
The invention discloses a method for quickly evaluating an influence degree of an organic acid level for alcohol metabolism of Maotai-flavour liquor. The method comprises the following steps of: S1: preparing mixed liquor; S2: adding 5[mu]L of 0.0075mg/ [mu]L of ethyl alcohol dehydrogenase liquid into the mixed liquor, after incubation is carried out, using a spectrograph to measure a light absorption value under a condition of 340mm wavelength to obtain a [delta]A determination tube; S3: defining the activity of ethyl alcohol dehydrogenase that at the temperature of 25 DEG C, an enzyme quantity required for each 1mL of acid solution to oxidize 1[mu] mol of coenzyme I in each 1min to generate 1[mu] mol of reduced nicotinamide adenine dinucleotide is one enzyme activity unit, and a following calculation formula is obtained: ADH([mu]mol/min/mL)=[([delta]A determination tube-[delta]A empty tube)/[epsilon]/d*V reaction total volume*10<6>]/V sample adding quantity/T; and S4: according to the above formula, analyzing the activity of the ethyl alcohol dehydrogenase under different concentrations of different organic acids. Through the above formula, the method disclosed by the invention calculates the activity of the ethyl alcohol dehydrogenase under different concentrations of different categories of organic acids, the influence degree of an organic acid level for after-drinking alcohol metabolism can be evaluated.
Description
technical field [0001] The invention relates to the technical field of brewing, in particular to a method for rapidly evaluating the degree of influence of organic acid levels on the alcohol metabolism of Maotai-flavor liquor. Background technique [0002] After alcohol is absorbed, it will enter the blood, and most of it will enter the liver through the blood. The liver can decompose about 90% of the alcohol, and about 10% of the alcohol will be excreted from the intestines, bladder and breath. The catabolism of alcohol in the liver mainly depends on two enzymes, namely alcohol dehydrogenase and acetaldehyde dehydrogenase. Among them, alcohol dehydrogenase (alcohol dehydrogenase, ADH) is the first key enzyme involved in the metabolism of alcohol in the body. ADH can reduce the bioavailability of the body to alcohol, alleviate the toxic effect of alcohol on the liver, brain and other organs, and prevent alcoholic intoxication. occurrence of liver disease. How to effectivel...
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