Unlock instant, AI-driven research and patent intelligence for your innovation.

Oomycete Inhibitors

A technology of oomycetes and lovastatin, applied in the direction of fungicides, biocides, biocides, etc.

Active Publication Date: 2021-10-26
SHENYANG AGRI UNIV
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no relevant report on the application of lovastatin in the control of oomycetes and the plant diseases caused by them

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Oomycete Inhibitors
  • Oomycete Inhibitors
  • Oomycete Inhibitors

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] The preparation of embodiment 1 lovastatin hydrolyzate

[0037] Add 400 mg of lovastatin to 20 mL of alkaline ethanol (15% v / v ethanol, 0.25% w / v NaOH), heat in a 60°C water bath until the lovastatin is completely dissolved and becomes active, and then stored at -20°C after filter sterilization.

Embodiment 2

[0038] Embodiment 2 lovastatin hydrolyzate bacteriostasis experiment

[0039] 1. Inhibit the growth of Phytophthora soybean mycelium

[0040] Add lovastatin hydrolyzate at final concentrations of 20, 40, and 80 μg / mL to V8 medium, inoculate Phytophthora soybean cake with a diameter of 5 mm in the center of the medium, and culture in the dark at 25°C for 3 days. By calculation, the growth rate of Phytophthora soybean mycelia on the control plate was 4.85 mm / d, and under the treatment of 20, 40, and 80 μg / mL lovastatin hydrolyzate, the growth rates were 4.50 mm / d, 4.07 mm / d and 3.30 mm / d, respectively. mm / d. Adding different concentrations of lovastatin hydrolyzate to the medium significantly reduced the mycelial growth rate of Phytophthora sojae ( figure 1 ).

[0041] 2. Inhibit the production of Phytophthora sojae sporangia

[0042] Lovastatin hydrolyzate was dissolved in water and prepared into solutions with concentrations of 20, 40, and 80 μg / mL respectively to stimulat...

Embodiment 3

[0050] Example 3 Lovastatin hydrolyzate reduces the incidence of soybean root rot caused by Phytophthora soybean

[0051] According to the results of indoor plate antibacterial experiments, 1 to 2 concentrations were selected for disease control experiments. Sow the susceptible soybean variety Hefeng 47 at 25°C under the conditions of 16h light / 8h dark, and grow for 2 weeks until the first pair of true leaves unfold. After inoculation of Phytophthora sojae hyphae, the aqueous solution containing lovastatin hydrolyzate (concentrations of 0, 40, and 80 μg / mL) was evenly sprayed on the soybean seedlings, and the incidence was counted after 3 days of cultivation. The results showed that all soybeans treated with control (concentration 0 μg / mL) were diseased and lodging, and the incidence rate was 100%; after treatment with lovastatin hydrolyzate at a final concentration of 40 μg / mL, the incidence rate dropped to 80%; especially 80 μg / mL After treatment with lovastatin hydrolyzate...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a novel oomycete inhibitor, the active ingredient of which is lovastatin hydrolyzate. The invention also provides the use of lovastatin in the prevention and treatment of plant oomycete diseases. By exogenously adding lovastatin to the culture medium, the growth rate of Phytophthora sojae hyphae can be significantly reduced, sporulation ability and pathogenicity ability can be inhibited, and it has similar growth inhibition effect on other plant pathogenic oomycetes. Lovastatin can be used as a potential inhibitor for the control of Phytophthora soybean and other oomycetes.

Description

technical field [0001] The invention relates to the field of plant disease control, in particular to an oomycete inhibitor. Background technique [0002] Oomycetes (oomycetes) is a large number of eukaryotic organisms, its shape is similar to fungi, filamentous growth, mycelium without septum. Oomycetes have long been classified as fungi, belonging to the subdivision Flagellate. However, they are quite different from fungi in the main components of the cell wall, the ploidy of chromosomes in the vegetative body, the types of asexual spores, and the size of the genome. Phylogenetic analysis at the molecular level found that oomycetes are closely related to plants such as diatoms, but far away from fungi. The new classification system classifies oomycetes into Stramenopila, Oomycota ( oomycota). Phytopathogenic oomycetes are an important group of pathogenic fungi, which can infect hundreds of plants and cause serious economic losses in agriculture and forestry. Phytophthor...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A01N37/36A01P3/00C07C67/29C07C69/30
CPCA01N37/36C07C67/29C07C2602/28C07C69/30
Inventor 杨新宇黄启凤姜雪孙慧颖梁月
Owner SHENYANG AGRI UNIV