Colorimetric detection method for nutrient activity of bait green algae
A detection method and bait technology, applied in the direction of microbial-based methods, biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problems of affecting biological growth, increasing the amount of residual bait, economic loss, etc., and achieve high detection efficiency , the effect of simple operation
Pending Publication Date: 2021-04-06
DALIAN OCEAN UNIV
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Problems solved by technology
Experiments have shown that when the bait green algae is in the exponential growth period with strong cell activity, the nutritional components such as total fat content and unsaturated fatty acid content are higher than those in the decay period. The low nutritional activity will affect the growth of organisms, and it will also increase the amount of residual bait, which will lead to the deterioration of water quality and cause economic losses
However, at present, for the management of bait green algae in the aquaculture industry, the number of algae is often measured by observing the living state through a microscope, empirical visual inspection or microscopic counting on a hemocytometer, and the nutritional activity of green algae cannot be accurately analyzed.
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[0016] A colorimetric detection method for the nutritional activity of bait green algae of the present invention is carried out according to the following steps:
[0017] a. Add 1.5g of anhydrous glucose to 150ml of the bait green algae liquid to be tested and shake well, seal it with a parafilm and then culture it under light at 22°C for 2 hours to obtain a culture medium. Oxygen; the algae liquid of green algae can be Chlorella pyrenoidesa (Chlorella pyrenoidesa) of the genus Chlorella, subcardiac flatweed ( Fetraselmis subcordiformis ) or Dunaliella salina ( Dunaliella salina );
[0018] b. Take 25ml of culture solution, add 5ml of iodine standard solution with a concentration of 0.05mol / L and 2ml of aqueous sodium hydroxide solution with a concentration of 2mol / L, shake well, seal and treat in dark for 15min;
[0019] c. Add 1ml of hydrochloric acid with a concentration of 6mol / L, 25ml of an aqueous solution of sodium thiosulfate with a concentration of 0.1mol / L and...
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The invention discloses a colorimetric detection method for nutritional activity of bait green algae, which is characterized in that the characteristic that green algae ingest glucose is utilized, glucose oxidation color development and an iodometry glucose measurement method are optimally combined, and detection conditions are limited to be higher alkalinity, so that algae cannot survive and settle in an alkaline environment, and then supernatant is taken for color development, so that the influence of the color of the green algae on glucose color development is avoided, the growth condition of the bait algae can be effectively reflected through color development, the cell density of the fresh algae liquid is rapidly compared, the nutritional activity of the green algae is determined, equipment such as culture experience and a blood cell counter is not needed, and the method has the advantages of simple operation, high detection efficiency and the like.
Description
technical field [0001] The invention relates to a detection method for the nutritional activity of bait green algae, in particular to a colorimetric detection method for the nutritional activity of bait green algae. Background technique [0002] Chlorophyta is the largest phylum among algae, with a wide variety of species and a wide distribution. About 90% of them are born in fresh water, and only about 10% of them live in seawater. Chlorella has a wide range of uses, and planktonic species such as Chlorella, Phytophthora, and Dunaliella are important bait for larvae of marine economic animals. Experiments have shown that when the bait green algae is in the exponential growth period with strong cell activity, the nutritional components such as total fat content and unsaturated fatty acid content are higher than those in the decay period. The low nutritional activity will affect the growth of organisms, and it will also increase the amount of residual bait, leading to deteri...
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IPC IPC(8): C12Q1/06C12R1/89
CPCC12Q1/06G01N2333/405
Inventor 赵文王宇魏杰张湾潘立峰
Owner DALIAN OCEAN UNIV