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Liquid chromatography-mass spectrometry analysis method of anthocyanin compounds in plants

An anthocyanin and analysis method technology, applied in the field of detection and analysis, can solve problems such as application limitations and affecting the effect of mass spectrometry analysis.

Inactive Publication Date: 2021-04-06
嘉兴迈维代谢生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because anthocyanins are only stable at low pH conditions (pH<3), and low pH values ​​will inhibit the ionization of electrospray mass spectrometry, which affects the effect of mass spectrometry analysis, resulting in limited application of this coupled technique

Method used

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  • Liquid chromatography-mass spectrometry analysis method of anthocyanin compounds in plants
  • Liquid chromatography-mass spectrometry analysis method of anthocyanin compounds in plants
  • Liquid chromatography-mass spectrometry analysis method of anthocyanin compounds in plants

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Embodiment 1

[0055] This embodiment provides a liquid chromatography-mass spectrometry analysis method for anthocyanins, which is tested using the parameter conditions in Table 1 and Table 2, including the following steps:

[0056] S1, according to the binding form of anthocyanin nucleus and sugar, deduce the anthocyanin compounds that may exist in plant samples.

[0057] There are six kinds of anthocyanin nuclei involved in the present invention, which are respectively Delphinidin, Cyanidin, Petunidin, Pelargonidin, and Paeoniflorin. (Peonidin), mallow pigment (Malvidin).

[0058] The binding forms of the anthocyanin core and sugar in the existing standard products are: the combination of the 3rd position with glucose, galactose, arabinose, rutinose, and malonyl glucose; or the combination of the 3rd position and the 5th position. glucose. It can be inferred that the six anthocyanin nuclei involved in the present invention all have the above-mentioned binding forms.

[0059] S2, using ...

Embodiment 2

[0074] This embodiment provides a liquid chromatography-mass spectrometry analysis method for anthocyanin compounds in orchids, comprising the following steps:

[0075] S1, preparing a plant sample solution to be tested.

[0076] Freeze-dried orchids, ground into powder. Add the extraction solvent to the powder at a weight-to-volume ratio of 1:20, vortex extract for 5 minutes, then ultrasonically centrifuge for 5 minutes, and take the supernatant; add the extraction solvent again to the precipitate, repeat the extraction for the second time, and combine the supernatant. Wherein, the extraction solvent is a mixed solvent containing 0.1% hydrochloric acid and water and methanol at a volume ratio of 2:8. Centrifuge and filter to obtain the orchid test solution.

[0077] S2, according to the chromatographic conditions shown in Table 1 and the mass spectrometry conditions shown in Table 2, the existing anthocyanin mixed standard solution and orchid test solution were tested on th...

Embodiment 3

[0080] This embodiment provides a liquid chromatography-mass spectrometry analysis method for anthocyanin compounds in grapes, comprising the following steps:

[0081] S1, preparing a plant sample solution to be tested.

[0082] Freeze-dried grapes, ground into powder. Add the extraction solvent to the powder at a weight-to-volume ratio of 1:20, vortex extract for 5 minutes, then ultrasonically centrifuge for 5 minutes, and take the supernatant; add the extraction solvent again to the precipitate, repeat the extraction for the second time, and combine the supernatant. Wherein, the extraction solvent is a mixed solvent containing 0.1% hydrochloric acid and water and methanol at a volume ratio of 2:8. Centrifuge and filter to obtain the orchid test solution.

[0083] S2, according to the chromatographic conditions shown in Table 1 and the mass spectrometry conditions shown in Table 2, the existing anthocyanin mixed standard solution and grape test solution were tested on the m...

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Abstract

The invention relates to a liquid chromatography-mass spectrometry analysis method of anthocyanin compounds in plants, the method comprises the following steps: preparing a to-be-detected plant sample solution, carrying out LC-MS / MS detection analysis under specific chromatographic conditions and mass spectrometric conditions, and determining whether the plant to be detected contains the anthocyanin compounds or not according to qualitative Q1 / Q3 ion pairs and retention time of the anthocyanin compounds. The liquid chromatography-mass spectrometry analysis method can be suitable for rapid analysis of anthocyanin substances in different plant samples.

Description

technical field [0001] The invention relates to the technical field of detection and analysis, in particular to a liquid mass spectrometry analysis method for anthocyanin compounds in plants. Background technique [0002] Anthocyanidin (anthocyanidin) is a kind of water-soluble pigment widely present in plants. It belongs to flavonoid compounds and is also the main coloring substance of plants. Moreover, it has certain nutritional and pharmacological effects, and has great application potential in food, cosmetics, medicine and other aspects. Experiments have shown that anthocyanins are the most powerful free radical scavenger found so far, and have health care functions, and have attracted more and more attention in recent years. Free anthocyanins are rare under natural conditions, mainly in the form of glycosides, often combined with one or more glucose, rhamnose, galactose, xylose, arabinose, etc. to form anthocyanins; and from plants The anthocyanins isolated from cyani...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/72
CPCG01N30/02G01N30/06G01N30/72
Inventor 许琳唐堂孟慧晓郑彬
Owner 嘉兴迈维代谢生物科技有限公司
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