Three-dimensional stent with communicating hollow structure and preparation method of three-dimensional stent

Abstract

The invention discloses a three-dimensional stent and a preparation method thereof. The preparation method comprises the steps of compounding cells and alginate hydrogel, and preparing 3D printing biological ink; constructing an active stent with a three-dimensional structure through 3D printing; performing multivalent cation cross-linking treatment on the active stent; transferring the active stent subjected to multivalent cation cross-linking treatment into a polylysine solution, and performing electrostatic complexing reaction treatment to obtain a stable structure; and chelating multivalent cations in the active stent after the electrostatic complexing reaction treatment, so that alginate in the active stent dissolves out. The three-dimensional stent has the communicating hollow structure, is high in operability, can carry various cells, medicines and bioactive factors through the communicating hollow structure besides meeting the physical characteristics of stability, a specific three-dimensional structure and the like required by a biological printing stent material, is beneficial to nutrient transportation and exchange, is beneficial to acceleration of tissue engineering repair, and is good in structural stability and excellent in pore controllability.

Description

technical field [0001] The invention relates to the technical field of tissue engineering repair, in particular to a three-dimensional scaffold with a connected hollow structure and a preparation method thereof. Background technique [0002] Tissue engineering is to use the principles and methods of engineering and life sciences to construct biologically active material complexes at the cellular and molecular levels. Permanent replacement and replacement to achieve functional reconstruction and regeneration of tissues and organs. In recent years, 3D printing, as an emerging technology, has attracted extensive attention from researchers in the field of tissue engineering. As an important branch of 3D printing technology, 3D bioprinting enables cells to grow and develop for a long time in a suitable material microenvironment through the precise three-dimensional spatial layout of biological materials, cells and bioactive factors, and realizes the bionic construction of human ...

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Problems solved by technology

[0005] However, the alginate bioprinted scaffolds of ionically cross-linked systems have the disadvantages of structural instability, such as the loss of multivalent cations will lead to structural damage.

Although the bioprinting effect of alginate can be improved by chemically modifying alginate (such as grafting photocrosslinking groups) or compounding with materials with other stable crosslinking methods, the simplicity of the preparation process, Convenience can be significantly affected

Although it has been reported that there is no polyvalent ion alginic acid 3D printing system, the carboxyl group of alginate and the amino group of polylysine can be amidated by using EDC / NSH chemical cross-linking solution to obtain a structurally stable scaffold, but the high Ink viscosity and EDC / NSH chemical cross-linking treatment will greatly reduce the survival rate of cells, so it is difficult to achieve bioprinting of loaded cells

In addition, most traditional alginate bioprinting scaffolds have a solid structure, and the diffusion resistance of the material is large, resulting in an internal nutritional environment that is not conducive to cell growth. How to give alginate bioprinting scaffolds more possibilities in design, such as forming a favorable The connected hollow structure of nutrient transport and exchange is also the direction of current research and exploration

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