Black dots remover and application thereof

Abstract

The invention discloses a black dots remover and application thereof, and relates to the field of biotechnology application. The black dots remover consists of the following components in parts by weight: 8-8.5 parts of ammonium chloride, 0.5-1.5 parts of potassium bicarbonate, 0.03-0.04 part of disodium EDTA and 995-1005 parts of double distilled water, and the application of an immunofluorescence mounting medium solution remover includes the following steps: A1, digesting adherent cells with 0.25% of pancreatin into a single-cell suspension; A2, performing centrifugal treatment on the single-cell suspension, re-depositing the single-cell suspension with the remover according to claim 1 after the centrifugal treatment, standing for 3-5min at room temperature, and then performing diluting with 10-15mL of PBS solution to obtain a diluent; and A3, carrying out centrifugal treatment on the diluent, carrying out re-suspension and re-culture with a medium after the centrifugal treatment to complete removal of the black dots. According to the black dots remover and the application thereof, the principle that the black dots are removed with the remover is different from the antibiotic sterilization principle, and the method is a physical method, so that sterilization is faster and more thorough, and the cost can also be lower.

Description

Technical field[0001]The present invention relates to the field of biotechnology applications, specific to a black gel deletion and its application thereof.Background technique[0002]Black cuvette pollution is a common contamination of laboratory cell culture. It is time to see a small black spot in the cell culture medium in 400 times microscope. The small black spots are sometimes dotted, sometimes small flakes, and the motion form is In situ vibration, like Brown movement, often known as a black gestium. The black gum is a commonly known as a pelletic substance formed after Nonchromacus infection, and a particulate matter formed by lysing cell residue. Norgobacterium has multiple resistance, and the current market is in the market to kill more antibiotics. However, due to the high resistance of the bacteria, it is often not fully removed, and often takes a certain period of time to kill. .Inventive content[0003]The present invention provides a problem with the problems in the prio...

AI Technical Summary

Problems solved by technology

Achromobacter has multi-drug resistance. At present, antibiotics are often used to kill black gum worms on the market. However, due to the high drug resistance of this bacteria, it is often impossible to completely remove such drug-resistant bacteria, and it often takes a certain period of treatment to kill them.

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