RNA bacteriostatic agent miRNA482a and crop pathogen inhibitor
A miRNA482a, inhibitor technology, applied in DNA/RNA fragments, fungicides, recombinant DNA technology, etc., can solve the problem of difficult occurrence of miRNA, and achieve the effect of strong specificity, good bacteriostatic effect and environmental friendliness
Active Publication Date: 2021-04-09
ZHEJIANG SCI-TECH UNIV
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Problems solved by technology
However, there are many differences between animal miRNAs and plant miRNAs, so it is generally believed that miRNAs in plants are difficult to appear in animals and even function
Method used
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Embodiment Construction
[0018] The present invention is further described below in conjunction with specific embodiment, but protection scope of the present invention is not limited thereto:
[0019] 1. Experiment preparation
[0020] (1) Synthesis of miRNA482a drug
[0021] The miRNA482a drug was synthesized at Gemma Gene (Shanghai, China). Each tube of miRNA482a drug 1OD, add RNase-free ddH according to the instructions 2 O, the final concentration of miRNA482a drug was 10 μM.
[0022] (2) Cultivation of Botrytis cinerea spores
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Abstract
The invention provides an RNA bacteriostatic agent miRNA482a, which has an RNA sequence shown in SEQ ID NO. 1. The invention also provides a crop pathogen inhibitor, which at least contains miRNA482a. Specifically, the inhibitor can be a spray containing miRNA482a, and effective prevention and treatment of botrytis can be realized by spraying. The solution applied with miRNA482a provided by the invention can significantly inhibit the germination of botrytis spores and the infection toxicity of botrytis to plants, and can be used for prevention and treatment of botrytis of vegetable crops.
Description
technical field [0001] The invention relates to the protection and treatment of botrytis cinerea, in particular to RNA bacteriostatic agent miRNA482a and a crop fungus inhibitor. Background technique [0002] Botrytis cinerea is a plant fungal disease caused by Botrytis cinerea. It can infect more than 200 kinds of fruits and vegetables such as tomatoes, strawberries, peppers, grapes, beans, and leeks. The host range is extremely wide. Botrytis cinerea is mainly transmitted by airflow, and the transmission speed is very fast, which makes the prevention and control of the disease very difficult in the process of tomato facility agriculture. With the continuous expansion of the planting area of protected cultivation agriculture, gray mold of tomatoes cultivated in protective facilities such as plastic greenhouses, greenhouses, and small arch sheds also commonly occurs. In general, the annual output reduction reaches 20% to 30%, and in severe cases it reaches more than 50%. ...
Claims
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IPC IPC(8): C12N15/113A01N57/16A01N25/06A01P3/00
CPCC12N15/113A01N57/16A01N25/06C12N2310/141
Inventor 吴方丽黄雕金伟波黄雅妮
Owner ZHEJIANG SCI-TECH UNIV
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