RNA bacteriostatic agent miRNA482a and crop pathogen inhibitor
A miRNA482a, inhibitor technology, applied in DNA/RNA fragments, fungicides, recombinant DNA technology, etc., can solve the problem of difficult occurrence of miRNA, and achieve the effect of strong specificity, good bacteriostatic effect and environmental friendliness
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[0018] The present invention is further described below in conjunction with specific embodiment, but protection scope of the present invention is not limited thereto:
[0019] 1. Experiment preparation
[0020] (1) Synthesis of miRNA482a drug
[0021] The miRNA482a drug was synthesized at Gemma Gene (Shanghai, China). Each tube of miRNA482a drug 1OD, add RNase-free ddH according to the instructions 2 O, the final concentration of miRNA482a drug was 10 μM.
[0022] (2) Cultivation of Botrytis cinerea spores
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