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Application of human thrombin-sensitive protein-1 in preparation of kit for predicting chemotherapy effect of intrahepatic cholangiocarcinoma

A technology for intrahepatic cholangiocarcinoma and sensitive proteins, which can be used in biological testing, measuring devices, material inspection products, etc., can solve the problems of less applied research, and achieve long overall survival and long progression-free survival

Pending Publication Date: 2021-04-09
上海柏运医疗器材有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still very few studies on the application of TSP1 in serological diagnostic markers
Moreover, there is no such research involving it as a marker for predicting chemotherapy sensitivity of intrahepatic cholangiocarcinoma (ICC)
[0005] Therefore, it is the first time that we use enzyme-linked immunosorbent assay (ELISA) to detect the expression of TSP1 in serum to predict the sensitivity of chemotherapy in patients with intrahepatic cholangiocarcinoma, which has not been reported in the literature before.

Method used

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  • Application of human thrombin-sensitive protein-1 in preparation of kit for predicting chemotherapy effect of intrahepatic cholangiocarcinoma
  • Application of human thrombin-sensitive protein-1 in preparation of kit for predicting chemotherapy effect of intrahepatic cholangiocarcinoma
  • Application of human thrombin-sensitive protein-1 in preparation of kit for predicting chemotherapy effect of intrahepatic cholangiocarcinoma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1: Screening and identification of TSP1

[0029] In the early stage, we screened gemcitabine-resistant human tumor xenograft models (Patient-Derived tumor Xenograft, PDX) using high-throughput general transcriptomes, and further used the genes with different expression levels in the drug-resistant models and sensitive models in these models, The 10 genes with the most significant changes were selected from the screened genes (see figure 1 ), and through further screening, it was found that among these genes, only the protein encoded by TSP1 could be detected in human serum, so it had the potential as a serum marker, so TSP1 was taken as the gene for research.

Embodiment 2

[0030] Example 2: Collection and processing of serum samples and detection of TSP1

[0031] (1) Serum sample collection: collect serum from patients with intrahepatic cholangiocarcinoma before chemotherapy.

[0032] (2) Serum sample processing: the collected peripheral blood samples were centrifuged at 3000 rpm at 4°C for 15 minutes, the supernatant was taken and packed into EP tubes, and stored at -80°C.

[0033] (3) Detection of TSP1 content in serum:

[0034] Prepare reagents and collect blood samples:

[0035] 1) The 10× specimen dilution is diluted 1:10 with distilled water (example: 1ml concentrated diluent + 9ml distilled water).

[0036] 2) Collect specimens: Serum, plasma (EDTA, citrate, heparin anticoagulant), cell culture supernatant, tissue homogenate, etc., should be tested as soon as possible, and stored at 2-8°C for 48 hours; longer time should be frozen (-20°C or -70°C) to avoid repeated freezing and thawing. Serum was diluted at least 100 times. Human sal...

Embodiment 3

[0049] Example 3: There are differences in the expression of TSP1 in serum between different patients

[0050] Through the detection method in Example 2, the expression level of TSP1 in TSP1 tissue and serum of patients with intrahepatic cholangiocarcinoma who have undergone surgery is detected, and it is found that TSP1 is different in different ICC patient tissues ( figure 2 A) and serum ( figure 2 B) There is a difference in the level, and the expression level of TSP1 in the serum is positively correlated with that in the tissue ( figure 2 C), which shows that TSP1 has the basic conditions and potential of clinical transformation as a serum marker.

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Abstract

The invention relates to the technical field of medical biological detection, and concretely relates to application of human thrombin-sensitive protein-1 in preparation of a kit for predicting the chemotherapy effect of intrahepatic cholangiocarcinoma. The application has the advantages that the protein chip is used for screening out the protein with expression difference in tissues of patients of an intrahepatic cholangiocarcinoma (ICC) chemotherapy drug-resistant group and an ICC chemotherapy sensitive group in the middle and advanced stages, the protein can be detected in serum, and it is found that the thrombin-sensitive protein-1 has the most obvious difference in two groups of patients and has a very high expression level in sensitive group patients. The invention provides the application of human thrombin-sensitive protein-1 as a serum marker for determining whether chemotherapy of an intrahepatic cholangiocarcinoma patient is sensitive or not. The expression level of the human thrombin-sensitive protein-1 is closely related to survival prognosis of the patient. The application provides a new clinical means for serological diagnosis.

Description

technical field [0001] The invention relates to the technical field of medical biological detection, in particular to the application of human thrombin-1 in the preparation of a kit for predicting the chemotherapy effect of intrahepatic cholangiocarcinoma. Background technique [0002] Proteins, as executors of life functions, play important functions in organisms, and proteins secreted outside the cell membrane are called secreted proteins or secreted proteins. Secreted proteins account for one-tenth of the human proteome. They play an important role in cell information transmission, and play roles in paracrine, autocrine, endocrine and other ways. Cell growth, differentiation, normal physiological functions of tissues and organs and The occurrence, development and outcome of diseases such as inflammation and tumor all depend on this kind of information transmission between cells. An important direction of secretory protein research is to use it as a serum marker for the d...

Claims

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Application Information

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IPC IPC(8): G01N33/574G01N33/68
CPCG01N33/57438G01N33/57488G01N33/6893
Inventor 周伟平张家宁杨远袁声贤
Owner 上海柏运医疗器材有限公司
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