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ZmGNAT19 gene SNP molecular marker related to corn leaf blight resistance and application

A technology of maize leaf spot and molecular markers, which is applied in the direction of DNA/RNA fragments, recombinant DNA technology, microbial measurement/inspection, etc., can solve the problems of reduced acetylation level, reduced resistance of rice blast bacteria, etc., and achieve simplified sequencing Effect

Active Publication Date: 2021-04-16
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Bo Ding et al. (Plant Cell, 2012) showed that overexpression of the sirtuin OsHDT701 gene in rice reduced the acetylation level of histone H4 in transgenic lines, resulting in reduced resistance to Magnaporthe oryzae
[0005] But so far, people's understanding of the role and function of the maize ZmGNAT19 gene is very limited, and there are few reports on the involvement of the ZmGNAT19 gene in the course of maize leaf spot disease.

Method used

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  • ZmGNAT19 gene SNP molecular marker related to corn leaf blight resistance and application
  • ZmGNAT19 gene SNP molecular marker related to corn leaf blight resistance and application
  • ZmGNAT19 gene SNP molecular marker related to corn leaf blight resistance and application

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Experimental program
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Embodiment 1

[0029] 1.1 Construction of GWAS population

[0030] The maize genome-wide association analysis population included 431 inbred lines with different genetic relationships. All the inbred lines are provided by the School of Plant Sciences of Jilin University and planted in the teaching and scientific research experiment base of the School of Plant Sciences of Jilin University (Lvyuan District, Changchun City, Jilin Province). The arrangement of the plots followed the random block design, and the blocks were repeated 3 times. The single-row plots had a row length of 3 m, a row spacing of 65 cm, and a plant-to-plant spacing of 20 cm. The field management measures were the same as those in the field.

[0031] 1.2 Collection of samples

[0032] At the 5-6 leaf stage after the emergence of corn seedlings in the plot, 3 seedlings were collected, cleaned and stored in a -20°C refrigerator.

[0033] 1.3 Disease classification of diseased leaves

[0034] The identification of disease g...

Embodiment 2

[0060] 2.1 Construction of sequencing library

[0061] (1) 200ng of genomic DNA was digested with the restriction endonuclease EcoRI, and purified and recovered by magnetic beads after complete digestion.

[0062] (2) The purified DNA after enzyme digestion was connected to Barcode adapters PI with T4 DNA Ligase, the connected product was purified and recovered by magnetic beads, and the qualified P1 primer label was recorded.

[0063] (3) All samples were mixed in equal proportions as required to form a total DNA mix, and the DNA was fragmented using covaris220. The fragment length of the fragmented DNA was about 200-500bp.

[0064] (4) After End Repair&dA-tailing, Adapter P2 is connected, and the connected product is purified and recovered by magnetic beads.

[0065] (5) Use the KAPA 2G Robust PCR Kit to amplify and enrich the adapter ligation products, use magnetic beads to purify and sort the PCR reaction products, and use 2% agarose gel electrophoresis to detect the qual...

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Abstract

The invention relates to a ZmGNAT19 gene SNP molecular marker related to corn leaf blight resistance and application, and belongs to the technical field of crop genetic breeding molecular marker application. The invention particularly relates to two extremely remarkale genes related to the leaf spot AUDPC (P<0.0001) ZmGNAT19 gene (Zm001d033620) 3'-UTR region on a corn chromosome 1 , an SNP molecular marker thereof and the application thereof. The ZmGNAT19 gene SNP molecular marker disclosed by the invention is obtained from whole genome association analysis of a maize inbred line group, and the nucleotide sequence of the ZmGNAT19 gene SNP molecular marker is shown as SEQ ID N01. The ZmGNAT19 gene SNP molecular marker disclosed by the invention can be used for auxiliary selection of corn leaf blight resistant varieties, the selection accuracy can be improved, and the cultivation process of disease-resistant varieties is accelerated.

Description

technical field [0001] The invention belongs to the technical field of application of molecular markers in crop genetic breeding, and in particular relates to a ZmGNAT19 gene SNP molecular marker related to resistance to maize leaf spot and its application. Background technique [0002] Northern corn leaf blight (Northern corn leaf blight) is a severe leaf blight disease caused by the fungus Exserohilum turcicum. Lesions can expand rapidly along the veins and are not limited by the veins, forming strips or long spindle-shaped lesions. In severe cases, the lesions merge, causing large areas of leaves to die, resulting in a significant reduction in yield. Generally, the large spot disease causes a 20% reduction in corn production in popular years, and more than 50% of the susceptible varieties in severe occurrence years. Breeding and promotion of maize leaf spot resistant varieties can not only reduce the incidence and yield loss, but also reduce the damage to the ecological ...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12N15/11
Inventor 胡军潘洪玉于汇琳杨凤肖坤钦何晓玥高超峰
Owner JILIN UNIV
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