Method capable of fully extracting algal polysaccharides and fucoxanthine in fucus marina

A fucoxanthin and seaweed polysaccharide technology, applied in the direction of organic chemistry, can solve the problems of lack of fucoxanthin extraction technology, fucoxanthin is not acid-resistant, and difficult to extract effective components, so as to improve the comprehensive utilization rate and economical efficiency. Benefits, fast extraction speed, and the effect of increasing corporate profits

Inactive Publication Date: 2021-04-20
SHANGHAI JAKA BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Fucoidan sulfate is not resistant to alkali, and fucoxanthin is not resistant to acid; and neither of them is resistant to temperature. The temperature resistance of fucoxanthin is worse than that of fucoxanthin, and it is easy to decompose under light conditions.
The existing process usually only extracts fucoidan sulfate or fucoxanthin from fucus, and currently there is no extraction process that can extract both fucoidan sulfate and fucoxanthin contained in fucus
In addition, the existing technology usually only extracts the active ingredients in the Fucus sporophyll, and it is difficult to extract the active ingredients contained in other parts of the Fucus algae

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] The embodiment of the present invention provides a method for comprehensively extracting fucoxanthin and seaweed polysaccharide from Fucus, comprising the following steps:

[0038] (1) Broken wall: get fresh Fucus and include all the algal bodies of sporophyll, dry the granules and sieve through a 50-mesh sieve, and soak the fine powder passing through the sieve with 5-10% calcium bicarbonate aqueous solution for 1 Wash for 1 hour to remove impurities on the surface, centrifuge (5000r / min, 10min) to drain the water on the surface of Fucus, then take out the Fucus and spread it on a plastic tray with a thickness of no more than 3 cm, and put it in -20°C Put the algae in the freezer below for more than 30 minutes to completely freeze the algae; then send the plastic tray into the microwave oven and use low-power continuous radiation for about 3 minutes until it is completely thawed and the thawing temperature is controlled not to exceed 25°C. The steps of freezing and mic...

Embodiment 2

[0046] The embodiment of the present invention provides a method for comprehensively extracting fucoxanthin and seaweed polysaccharide from Fucus, comprising the following steps:

[0047] (1) Wall breaking: wall breaking: get all the algal bodies of fresh Fucus algae containing sporophylls, dry and pulverize and sieve through a 70-mesh sieve, and use 5-10% calcium bicarbonate aqueous solution to get the fine powder passing through the sieve holes Soak for 30 minutes to wash and remove impurities on the surface, centrifuge (5000r / min, 15min) to drain the water on the surface of Fucus, then take out Fucus and lay them on a glass surface dish, the thickness of the layer is not more than 2 cm, and place The sub-zero environment freezes the algal body until it is completely frozen; then the glass watch glass is sent to a microwave oven and irradiated in a pulsed power of 1000 watts, each microwave irradiation time is 5 seconds, with an interval of 10 seconds, and the thawing is comp...

Embodiment 1

[0061] Take 10 grams of the Fucus powder sample obtained in step (1) of Example 1 or Example 2 after drying, and add 50 milliliters of absolute ethanol.

[0062] Using a solid phase extraction apparatus, the extraction temperature does not exceed 45 ° C, and the sample is extracted for more than 2 hours in a dark environment. Weigh the clear extract after removing the solid phase. Adopt high performance liquid chromatography to measure the content of fucoxanthin in the sample extract, calculate the theoretical content of fucoxanthin according to formula I, and calculate the yield of fucoxanthin according to formula II ("yield" is actual Yield / theoretical yield, "yield" is actual yield / raw material quality).

[0063] Fucoxanthin theoretical content = 100% * (fucoxanthin content in the extract * extract quality) / sample quality (Formula I)

[0064] Yield=100%*(fucoxanthin content*product weight / raw material quality in the product) / fucoxanthin theoretical content (Formula II) ...

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Abstract

The invention belongs to the technical field of marine natural product extraction, and particularly relates to a process capable of fully extracting algal polysaccharides and fucoxanthine in fucus marina so as to maximize the economic value of the fucus marina. The method is also suitable for comprehensively extracting fucoxanthin, fucosan sulfate, biosaccharide gum and other algal polysaccharides from other types of brown algae. The invention discloses a method for comprehensively extracting fucoxanthin and algal polysaccharides from fucus marina. The method comprises the following steps: (1) breaking walls; (2) extracting fucoxanthin; (3) extracting algal polysaccharides; and (4) purifying the algal polysaccharide crude product. The method has the advantages of simple operation, high yield and purity of fucoxanthin and fucosan sulfate, and the like.

Description

technical field [0001] The invention belongs to the technical field of marine natural product extraction, and specifically refers to a method for comprehensively extracting fucoxanthin and seaweed polysaccharide from fucus. Background technique [0002] Fucus vesiculosus (Latin scientific name Fucus vesiculosus) is a brown algae that grows attached to rocks on the shore. Its algae are dark brown, bifurcated branches, and flat horn-like leaves. Most are distributed in tropical and subtropical oceans, and some are distributed in frigid zones. In my country, it is distributed in Liaoning, Shandong and other coastal areas. The compound leaves of Fucus are covered with a slime that keeps the seaweed from dying when the tide recedes. The algae are perennial, bifurcated, without a central axis. Sperm sacs and oocysts grow in the reproductive fossa (or sporophyll) on the enlarged reproductive receptacle at the top of the algae. The base has disc-shaped holders, flat strip-shape...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D303/32C08B37/00
Inventor 田军刘青卢伊娜
Owner SHANGHAI JAKA BIOTECH CO LTD
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