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A method for improving enzymatic hydrolysis effect of lignocellulosic raw materials

A technology of lignocellulose and raw material enzymes, applied in the direction of fermentation, etc., can solve the problem that the enzymatic hydrolysis efficiency of bamboo chips and poplar sawdust is not up to expectations, and achieve the effect of reducing non-productive adsorption

Active Publication Date: 2021-06-22
NANJING FORESTRY UNIV
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  • Description
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Problems solved by technology

Similarly, according to previous research results, even if the intensity of dilute acid pretreatment is increased, the enzymatic hydrolysis efficiency of bamboo chips and poplar wood chips is still not as expected

Method used

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  • A method for improving enzymatic hydrolysis effect of lignocellulosic raw materials
  • A method for improving enzymatic hydrolysis effect of lignocellulosic raw materials
  • A method for improving enzymatic hydrolysis effect of lignocellulosic raw materials

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Embodiment 1

[0027] A method for improving the enzymatic hydrolysis effect of lignocellulosic raw materials, the steps are as follows:

[0028] (1) Weigh poplar wood chips and bamboo chips with an absolute dry mass of 10 g, and treat them with 0.5% (m / v) sulfuric acid aqueous solution at a solid-to-liquid ratio of 1:10 (g / g) at 170 °C for 1 h.

[0029] (2) After dilute acid pretreatment, solid-liquid separation was carried out, and the obtained solid residue was washed with distilled water to neutrality, and filtered to obtain poplar wood chips and bamboo chips after dilute acid pretreatment. The main component analysis is shown in Table 1.

[0030] (3) Weigh the above dilute acid pretreated material and microcrystalline cellulose with a dry weight of 1.000 g in 125 mL enzyme hydrolysis bottles, and add 1 mL of 1 mol / L citric acid to each enzyme hydrolysis bottle Add the expired milk protein solution with the dosage of 50, 100, and 150 mg / mg cellulose, 20 μL of 10 g / L tetracycline solution...

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Abstract

The invention discloses a method for improving the enzymatic hydrolysis effect of lignocellulose raw materials, which belongs to the technical field of lignocellulose raw materials pretreatment and enzymatic hydrolysis. The method is to carry out dilute acid pretreatment on the lignocellulose raw material in an acidic aqueous solution. After the pretreatment, the solid-liquid separation is completed to obtain the filter residue; then an adsorption system containing an aqueous solution of expired milk protein is added to the residue for adsorption, and fiber Sulfase, which enzymatically hydrolyzes the filter residue in the enzymatic hydrolysis system, realizes the enzymatic hydrolysis process of lignocellulosic raw materials. Before the enzymatic hydrolysis operation, the addition of expired milk protein effectively reduced the non-specific adsorption between cellulase and lignin, which was beneficial to the hydrolysis of cellulase. Compared with the existing research results, the enzymatic hydrolysis yield of the present invention is significantly improved, reaching 94.6%. It also provides a new idea for the recycling of expired milk.

Description

technical field [0001] The invention belongs to the technical field of lignocellulose raw material pretreatment and enzymatic hydrolysis, and in particular relates to a method for improving the enzymatic hydrolysis effect of lignocellulose raw material. Background technique [0002] With the rapid development of global economy and technology, people's demand for resources is increasing, especially fossil resources. However, insufficient supply of fossil resources and many climate problems such as global warming are caused by the massive use of fossil fuels. Therefore, it is urgent to find an alternative clean and green fuel, such as biofuel, which is a promising energy source. Lignocellulosic feedstock is an inexpensive, renewable and sustainable resource for biofuel production. Biomass resources mainly include forestry residues, grasses, agricultural wastes, etc., which are widely distributed and abundant in the world. Both bamboo and poplar are promising resources for b...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/14C12P19/02
Inventor 黄曹兴林文倩勇强赖晨欢李鑫凌喆
Owner NANJING FORESTRY UNIV