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Sulfhydrylated ssDNA probe-functionally modified MOFs compound material as well as preparation method and application thereof

A sulfhydrylation and functionalization technology, used in material excitation analysis, fluorescence/phosphorescence, etc., can solve the problems of complex manufacturing process, low sensitivity that cannot meet actual detection requirements, and heavy instrument detection equipment.

Active Publication Date: 2021-04-20
XI AN JIAOTONG UNIV
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  • Abstract
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Problems solved by technology

The SPR optical fiber sensor mainly realizes the detection and analysis of the target analysis characteristics by measuring the change of the transmitted optical power after plasma modulation, and its manufacturing process is complicated.
Graphene-based sensors utilize the flexibility and good electrical conductivity of graphene and the rich surface chemical properties and high absorption rate of bacterial cellulose nanofibers to realize the detection of liquid alcohol, but their low sensitivity cannot meet the actual detection requirements.
Liquid chromatography / flame ionization detection method has higher sensitivity and accuracy, but its heavy instrument detection equipment and complicated operation technology are the main reasons that limit its wide application

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  • Sulfhydrylated ssDNA probe-functionally modified MOFs compound material as well as preparation method and application thereof
  • Sulfhydrylated ssDNA probe-functionally modified MOFs compound material as well as preparation method and application thereof
  • Sulfhydrylated ssDNA probe-functionally modified MOFs compound material as well as preparation method and application thereof

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Embodiment Construction

[0054] The present invention will be described in further detail below in conjunction with the accompanying drawings and specific embodiments, but the present invention is not limited.

[0055] figure 1 Among them, the thiolated ssDNA probe of the present invention-functionalized modified MOFs composite material, including ZrCl 4 、C 8 h 7 NO 4 and C 7 h 6 o 2 MOFs material UiO-66-NH synthesized by other substances 2 , bifunctional cross-linking reagent Sulfo-SMCC, thiolated ssDNA probe, fluorescent dye RhoB.

[0056] A kind of sulfhydryl ssDNA probe-functionalized modified MOFs composite material, the material includes MOFs material UiO-66-NH 2 , bifunctional cross-linking reagent Sulfo-SMCC (Sulfosuccinimidyl4-(Nmaleimidomethyl)cyclohexane-1–carboxylate), sulfhydrylated ssDNA probe and fluorescent dye RhoB (Rhodamine B). Thiolated ssDNA probes were covalently modified to MOFs material UiO-66-NH by bifunctional cross-linking reagent Sulfo-SMCC 2 surface. Using MOFs ...

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Abstract

The invention discloses a sulfhydrylated ssDNA probe-functionally modified MOFs composite material as well as a preparation method and application of the MOFs composite material. The composite material comprises an MOFs material UiO-66-NH2, a bifunctional cross-linking reagent Sulfo-SMCC, a sulfhydrylated ssDNA probe, fluorescent dye RhoB and the like. The method comprises the following steps that: a sulfhydrylated ssDNA probe-functionally modified MOFs composite material is manufactured; the TEM diagram and the Zeta potential of the composite material are characterized; the composite material is used for specific detection of liquid alcohols; detection limit detection and repeatability experiments are carried out by taking ethanol as a representative. Results show that the sulfhydrylated ssDNA probe-functionally modified MOFs composite material realizes simple, effective, low-cost and high-sensitivity detection of liquid alcohols, and a new research direction is provided for development of portable detection equipment of the liquid alcohols.

Description

technical field [0001] The invention relates to the field of alcohol detection, in particular to a sulfhydrylated ssDNA probe-functionalized modified MOFs composite material and its preparation method and application. Background technique [0002] The detection of alcohols in solution is of great significance in the fields of forensic science, drug screening, environment and food safety. Current methods for the detection of liquid alcohols are surface plasmon resonance (SPR) fiber optic sensors, graphene-based sensors, and liquid chromatography / flame ionization detection. The SPR optical fiber sensor mainly realizes the detection and analysis of the target analysis characteristics by measuring the change of the transmitted optical power after plasma modulation, and its manufacturing process is complicated. Graphene-based sensors utilize the flexibility and good electrical conductivity of graphene as well as the rich surface chemical properties and high absorptivity of bacte...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
Inventor 吴春生陈雅婷田玉兰朱平刘淑阁杜立萍陈炜
Owner XI AN JIAOTONG UNIV
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