Immunosensing carrier and kit for syphilis antibody detection, and preparation method of carrier

A technology of antibody detection and immune sensing, applied in the field of immune detection, can solve the problems of TP antibody detection speed and accuracy to be improved, and achieve the effect of high-throughput automatic quantitative detection and accurate detection

Active Publication Date: 2021-04-20
JIHUA LAB
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of the above-mentioned deficiencies in the prior art, the object of the present invention is to provide an immunosensing carrier, kit and preparation method for syphilis antibody detection, aiming at solving the problem that the speed and accuracy of the existing TP antibody detection need to be improved

Method used

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  • Immunosensing carrier and kit for syphilis antibody detection, and preparation method of carrier
  • Immunosensing carrier and kit for syphilis antibody detection, and preparation method of carrier
  • Immunosensing carrier and kit for syphilis antibody detection, and preparation method of carrier

Examples

Experimental program
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Embodiment 1

[0084] 1) Pretreatment silk film

[0085] Take an appropriate amount of 50g silkworm cocoons, cut the silkworm cocoons, wash and soak in water for 1h, and bathe in water at 60°C for 2h, soak and wash to remove impurities on the surface of the silkworm cocoons. The PBS buffer solution with a concentration of 0.01M and a pH of 7.2, the tris-Hcl buffer solution with a concentration of 0.01M and a pH of 7.2, and the borate buffer solution with a concentration of 0.01M and a pH of 7.2 were prepared according to the ratio of 1:1:1. Mix by volume to make a mixing buffer. Soak the silkworm cocoons with 300ml of mixing buffer, wash and stir for 30min. Add 120ml of a mixture of glycerol and enzyme, and incubate at 30°C for 30min while stirring. The mixed solution of glycerin and enzyme comprises 40 parts of glycerin, 20 parts of lipase and 20 parts of papain in terms of parts by mass. Add 1.8 g of protease inhibitor PMSF, act for 5 minutes, filter with a 200-mesh sieve, discard the f...

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Abstract

The invention discloses an immunosensing carrier and a kit for syphilis antibody detection, and a preparation method of the carrier. The immunosensing carrier for syphilis antibody detection comprises a porous membrane; the porous membrane is connected with a specific anti-silk membrane antibody; the specific anti-silk membrane antibody is connected with a cross-linked antibody; and the cross-linked antibody is connected with the TP specific antibody and the recombinant TP antigen. The immunosensing carrier and the kit for syphilis antibody detection provided by the invention can realize rapid and accurate detection of the TP antibody.

Description

technical field [0001] The invention relates to the field of immune detection, and mainly relates to an immune sensing carrier, a kit and a preparation method for detecting syphilis antibodies. Background technique [0002] In recent years, the incidence of syphilis in my country has shown an obvious upward trend, posing a serious threat to public health and clinical blood transfusion. Accurate screening of clinical blood transfusion and blood products is helpful to control the spread of TP. Therefore, it is very important to establish a rapid and highly sensitive detection method for syphilis at the protein or nucleic acid level for the prevention, treatment and prognosis of the disease. At present, there are mainly pathogenic detection and serological detection methods in clinical practice. Serological detection of TP antibody is an important method for clinical diagnosis of syphilis, including venereal laboratory research test (vDRL), rapid plasma reagin ring card test ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/571G01N33/68G01N33/531
Inventor 李勇王红梅段生宝丁少华魏双施田晶晶陈晔洲吴斐然杜子谦
Owner JIHUA LAB
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