Preparation method of polyphenol-protein nanoparticles for relieving colitis

A nanoparticle and colitis technology, applied in the direction of nanotechnology, nanotechnology, nanomedicine, etc., can solve the problems of loss of activity, limit wide application, poor stability, etc., and achieve the effect of increasing the loading rate

Pending Publication Date: 2021-04-23
HAIKOU EXPERIMENTAL STATION CHINESE ACAD OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, polyphenols are very sensitive to environmental changes such as light, heat, and pH, have poor stability, and

Method used

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  • Preparation method of polyphenol-protein nanoparticles for relieving colitis
  • Preparation method of polyphenol-protein nanoparticles for relieving colitis
  • Preparation method of polyphenol-protein nanoparticles for relieving colitis

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preparation example Construction

[0026] The first aspect of the present invention provides a method for preparing polyphenol-protein nanoparticles for relieving colitis, comprising the following steps:

[0027] (1) Dissolve the protein in the buffer solution, stir at room temperature until the protein is completely hydrated to obtain a protein solution, and store it at 0-5°C for later use;

[0028] (2) performing heat treatment after the protein solution is filtered through the membrane, and then cooling to obtain a heat-treated protein solution;

[0029] (3) Dissolve the polyphenols in the solution and store them away from light; sequentially add them to the heat-treated protein solution at intervals of 0.5-2 hours to obtain a complex solution; disperse with a high-speed disperser and homogenize several times to obtain .

[0030] step 1)

[0031] In the present invention, the step (1) specifically includes: dissolving the protein in the buffer solution, stirring at room temperature until the protein is co...

Embodiment 1

[0078] Embodiment 1 provides a kind of preparation method of the polyphenol (quercetin)-beta-lactoglobulin nanoparticle that alleviates colitis, is prepared by the preparation method comprising the following steps:

[0079] (1) Dissolve protein in sodium phosphate buffer (40mmol / L, pH 6), add sodium azide as preservative to avoid microbial growth. Stir continuously at 300 rpm / min for 100 min at room temperature to ensure complete hydration of the protein, store the protein solution at 4°C overnight, and filter the sample through a 0.2 μm filter membrane to remove protein aggregates and impurities. Put the protein solution into a stoppered glass tube, heat it in a water bath at 60°C for 12 minutes, take it out and cool it in ice water for 10 minutes, and the concentration of the obtained protein solution is 10 mg / mL;

[0080] (2) According to the hydrophilicity of the polyphenols, they were respectively dissolved in sodium phosphate buffer solution, absolute ethanol or 70% etha...

Embodiment 2

[0083] Embodiment 2 provides a kind of preparation method of the polyphenol (ferulic acid, quercetin)-beta-lactoglobulin nanoparticle that alleviates colitis, is prepared by the preparation method comprising the following steps:

[0084] (1) Dissolve protein in sodium phosphate buffer (40mmol / L, pH 6.5), add sodium azide as a preservative to prevent microbial growth. Stir continuously at 300 rpm / min for 110 min at room temperature to ensure complete hydration of the protein, store the protein solution at 4°C overnight, and filter the sample through a 0.2 μm filter membrane to remove protein aggregates and impurities. Put the protein solution into a stoppered glass tube, heat it in a water bath at 60°C for 10 minutes, take it out and cool it in ice water for 10 minutes, and the concentration of the obtained protein solution is 10mg / mL;

[0085] (2) According to the hydrophilicity of the polyphenols, they were respectively dissolved in sodium phosphate buffer solution, absolute ...

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Abstract

The invention relates to the technical field of food biology, in particular to a preparation method of polyphenol-protein nanoparticles for relieving colitis. The invention provides the preparation method of the polyphenol-protein nanoparticles for relieving colitis. The preparation method comprises the following steps: (1) dissolving protein in a buffer solution, performing stirring at room temperature until the protein is completely hydrated to obtain a protein solution, and storing at 0-5 DEG C for later use; (2) filtering the protein solution through a filter membrane, then performing heat treatment, and then performing cooling to obtain a heat-treated protein solution; (3) dissolving polyphenol in the solution, and performing storing in a dark place; sequentially adding the protein solution subjected to heat treatment at intervals of 0.5-2h to obtain a compound solution; and performing dispersing by using a high-speed disperser, and performing homogenizing for several times to obtain the product. A polyphenol-protein nano-emulsion prepared by the method provided by the invention can protect polyphenol from being influenced by digestion of gastric juice and intestinal juice, so that the polyphenol effectively reaches colon, and the effects of resisting oxidation and inflammation and relieving colitis symptoms are achieved.

Description

technical field [0001] The invention relates to the field of food biotechnology, in particular to a preparation method of polyphenol-protein nanoparticles for alleviating colitis. Background technique [0002] Plant-derived polyphenols, such as ferulic acid (hydrophilic), quercetin (hydrophobic) and vanillic acid (amphiphilic), resveratrol, etc. have antibacterial, anti-inflammatory, anti-oxidant, free radical scavenging, etc., such as Potato tannin has astringent properties, can shrink the capillaries of the wound, and has local hemostatic effect. It can be used orally to treat gastrointestinal bleeding, ulcers and watery diarrhea. In addition, yam tannin can interact with protein and coagulate the protoplasm in microorganisms, so it has antibacterial effect. However, polyphenols are very sensitive to environmental changes such as light, heat, and pH, have poor stability, and are easily oxidized to form quinones that lose their activity, which limits their wide application...

Claims

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Application Information

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IPC IPC(8): A23L33/105A23L33/19A23L33/17A61K47/69A61P1/00A61P39/06A61P29/00B82Y5/00A61K31/05
CPCA23L33/105A23L33/19A23L33/17A61K47/6931A61P1/00A61P39/06A61P29/00B82Y5/00A61K31/05A23V2002/00A23V2250/2132A23V2250/54252A23V2250/54242A23V2250/54244A23V2250/5436A23V2200/32
Inventor 杨旸盛占武艾斌凌郑丽丽郑晓燕校导钟爽
Owner HAIKOU EXPERIMENTAL STATION CHINESE ACAD OF TROPICAL AGRI SCI
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