Complex special enzyme for efficient sea cucumber peeling and preparation method and application thereof
A special enzyme and sea cucumber technology, which is applied in the field of high-efficiency sea cucumber peeling and compounding special enzymes and preparation, can solve the problems of poor controllability of removing sea cucumber skin, loss of nutrients, and low yield.
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Embodiment 1
[0040] A preparation method for high-efficiency sea cucumber peeling compound special enzyme, comprising the following steps:
[0041]S1: Extraction of papain: collect papaya pulp, mix papaya pulp and water according to the weight ratio of 1:5, wait for the papaya pulp to be fully dissolved and extract for 30 minutes, filter, and the filtrate will be removed by microfiltration bio-organic membrane within 30 minutes Impurities are sterilized until the total number of food-grade colonies is ≤1×10 4 cfu / g, then concentrated to 70% concentration through ultrafiltration biofilm, freeze-dried to obtain papain with a purity of ≥ 98%, crushed to 90% and can pass through an 80-mesh standard sieve in powder form for future use;
[0042] S2: Extract bromelain: mix pineapple juice and water at a weight ratio of 1:5, and pass the mixed solution through microfiltration bio-organic membrane to remove impurities and bacteria within 30 minutes until the total number of food-grade colonies is ≤...
Embodiment 2
[0052] A preparation method for high-efficiency sea cucumber peeling compound special enzyme, comprising the following steps:
[0053] S1: Extraction of papain: collect papaya pulp, mix papaya pulp and water according to the weight ratio of 1:5, wait for the papaya pulp to be fully dissolved and extract for 30 minutes, filter, and the filtrate will be removed by microfiltration bio-organic membrane within 30 minutes Impurities are sterilized until the total number of food-grade colonies is ≤1×10 4 cfu / g, then concentrated to 70% concentration through ultrafiltration biofilm, freeze-dried to obtain papain with a purity of ≥ 98%, crushed to 90% and can pass through an 80-mesh standard sieve in powder form for future use;
[0054] S2: Extract bromelain: mix pineapple juice and water at a weight ratio of 1:5, and pass the mixed solution through microfiltration bio-organic membrane to remove impurities and bacteria within 30 minutes until the total number of food-grade colonies is ...
Embodiment 3
[0064] A preparation method for high-efficiency sea cucumber peeling compound special enzyme, comprising the following steps:
[0065] S1: Extraction of papain: collect papaya pulp, mix papaya pulp and water according to the weight ratio of 1:5, wait for the papaya pulp to be fully dissolved and extract for 30 minutes, filter, and the filtrate will be removed by microfiltration bio-organic membrane within 30 minutes Impurities are sterilized until the total number of food-grade colonies is ≤1×10 4 cfu / g, then concentrated to 70% concentration through ultrafiltration biofilm, freeze-dried to obtain papain with a purity of ≥ 98%, crushed to 90% and can pass through an 80-mesh standard sieve in powder form for future use;
[0066] S2: Extract bromelain: mix pineapple juice and water at a weight ratio of 1:5, and pass the mixed solution through microfiltration bio-organic membrane to remove impurities and bacteria within 30 minutes until the total number of food-grade colonies is ...
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