Antihypertensive pharmaceutical composition as well as preparation method and application thereof

Inactive Publication Date: 2021-05-04
SHANDONG UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is currently a lack of antihypertensive products with clear active ingredients and definite curative effects of traditional Chinese medicine

Method used

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  • Antihypertensive pharmaceutical composition as well as preparation method and application thereof
  • Antihypertensive pharmaceutical composition as well as preparation method and application thereof
  • Antihypertensive pharmaceutical composition as well as preparation method and application thereof

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Experimental program
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Effect test

preparation example Construction

[0020] The present invention also provides a method for preparing the pharmaceutical composition described in the above technical solution, comprising the following steps: mixing isorhynchophylline and sinapine thiocyanate to obtain the pharmaceutical composition.

[0021] In the present invention, the preparation method of the isorhynchophylline preferably includes: cold-soaking Uncaria in 70% ethanol aqueous solution by volume, filtering the filtrate, concentrating under reduced pressure until it has no alcohol smell, and adjusting the pH value to 1.0~3.0, more preferably 2.0, to obtain the first extract, the first extract is mixed with chloroform according to (1~2):(1~2), more preferably 1:1 volume ratio and extract , take the water phase to adjust the pH value to 10.0~12.0, more preferably 11.0, to obtain the second extract, and the second extract is according to (1~2):(1~2), more preferably 1:1 volume Mix with chloroform, extract, take the organic phase, recover under red...

Embodiment 1

[0030] Synergistic protective effects of isorhynchophylline and sinapine thiocyanate against angiotensin II-induced HUVECs cell injury

[0031] Experimental Materials

[0032] Experimental cells: human umbilical vein endothelial cells (Human Umbilical Endothelial Cells, HUVECs), purchased from ScienCell (USA), the 4th to 7th passages were used for experiments.

[0033] Experimental drugs: Isorhynchophylline (IRN), sinapinethiocyanate (Sinapinethiocyanate, ST), and valsartan (Valsartan, Val) were purchased from Chengdu Chromar Biotechnology Co., Ltd. Angiotensin II (Angiotensin II, Ang II) was purchased from Beijing Suolaibao Technology Co., Ltd.

[0034]Experimental reagents: nitric oxide (NO) (product number: A013-2-1) was purchased from Nanjing Jiancheng Bioengineering Institute, endothelin 1 (ET-1) enzyme-linked immunoassay (ELISA) kit (product number: JYM0710Hu) was purchased from The Cell Proliferation and Toxicity Detection (CCK-8) Kit (Product No.: MAO218-1) was purch...

Embodiment 2

[0054] Antihypertensive Pharmacodynamic Experiment of Isorhynchophylline and Sinapine Thiocyanate Composition on Spontaneously Hypertensive Rats (SHR)

[0055] Experimental Materials

[0056] Experimental animals: the model group was SPF grade 8-week-old male spontaneously hypertensive rats (SHR), with a body weight of 180-200 g, and the normal group was SPF-grade 8-week-old male Wistar Kyoto rats (WKY), with a body weight of 180-200 g. All were purchased from Beijing Weitong Lihua Experimental Animal Technology Co., Ltd. (SCXK (Beijing): 2016-0005), and were kept in an animal laboratory at a room temperature of 20-25°C and a humidity of 50-65%.

[0057] Experimental drugs: isorhynchophylline and sinapine thiocyanate, both purchased from Chengdu Cromar Biotechnology Co., Ltd., production batch number 20201011, specification 2g / bottle; valsartan, production batch number x1041 H2020723, Beijing Novartis Pharmaceutical Co., Ltd. product.

[0058] Experimental equipment: BP-98A ...

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Abstract

The invention relates to an antihypertensive pharmaceutical composition as well as a preparation method and application thereof, and belongs to the technical field of antihypertensive drugs. The pharmaceutical composition disclosed by the invention is prepared from isorhynchophylline and sinapine thiocyanate. The pharmaceutical composition is low in price, easy to obtain, definite in curative effect, low in toxic and side effect and capable of preventing and treating hypertension in a multi-target mode.

Description

technical field [0001] The invention relates to the technical field of antihypertensive drugs, in particular to an antihypertensive pharmaceutical composition, a preparation method and application thereof. Background technique [0002] Hypertension is a common chronic disease and an important risk factor for the occurrence and death of cardiovascular diseases. It has high morbidity, disability, and fatality rates. It seriously consumes medical and social resources, causing serious harm to the entire family and society. The heavy burden has also brought great challenges to the public health of all mankind. The application of antihypertensive western medicine can effectively control the blood pressure level of patients, but it has the characteristics of large side effects and it is difficult to improve the quality of life of patients. In terms of prevention and treatment of hypertension, traditional Chinese medicine can not only effectively lower blood pressure, but also sign...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/438A61K31/216A61P9/12
CPCA61K31/438A61K31/216A61P9/12A61K31/437A61K36/74A61K2236/00A61K2300/00
Inventor 李运伦王小明蒋海强蔺琳冯俊杰
Owner SHANDONG UNIV OF TRADITIONAL CHINESE MEDICINE
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