Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method of lentinus edodes extract containing NMN

A technology of shiitake mushroom extract and shiitake mushroom, applied in food science, function of food ingredients, food ultrasonic treatment, etc., can solve the problems of inability to extract NMN and low efficiency of NMN

Pending Publication Date: 2021-05-11
金华贝塔生物科技有限公司
View PDF1 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The present invention proposes a preparation method of NMN-containing shiitake mushroom extract, which solves the existing problem of being unable to extract NMN from shiitake mushrooms for commercialization, and the existing low efficiency of extracting NMN from natural food

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of lentinus edodes extract containing NMN
  • Preparation method of lentinus edodes extract containing NMN
  • Preparation method of lentinus edodes extract containing NMN

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] A preparation method of a shiitake mushroom extract containing NMN, comprising:

[0038] Add 200 kg of fresh shiitake mushrooms (picked in the first three days) to 600 liters of 75% ethanol, sonicate for 30 minutes, soak for 24 hours, filter, carry out molecular distillation (control temperature 83°C, vacuum degree below 30bar), remove a large amount of ethanol and water , The concentrated solution was left to stand for more than 3 hours, the supernatant was taken, and freeze-dried to obtain 3.126 kg of powder. Detected by HPLC, the NMN content was 3.2%. The molecular weight of NMN in it was 334 as detected by liquid chromatography-mass spectrometry.

Embodiment 2

[0040] A preparation method of a shiitake mushroom extract containing NMN, comprising:

[0041] Add 1000 kg of fresh shiitake mushrooms (picked five days ago) with 3000 liters of 70% ethanol, ultrasonic for 33 minutes, soak for 12 hours, filter, molecular distillation (control temperature 85°C, vacuum below 30bar), remove a large amount of ethanol and water, Let the concentrated solution stand for more than 3 hours, take the supernatant, and spray dry it with a spray dryer with a product cooling collection system (inlet air temperature 150° C.) to obtain 15.523 kg of powder. As detected by HPLC, the NMN content was 3.3%. Liquid-mass spectrometer detection, wherein the molecular weight of NMN is 334.

Embodiment 3

[0043] A preparation method of a shiitake mushroom extract containing NMN, comprising:

[0044] Fresh shiitake mushrooms (picked two days ago) 2,000 kg plus 6,000 liters of 73% ethanol, ultrasonicated for 28 minutes, soaked for 36 hours, filtered, molecularly distilled (control temperature 80°C, vacuum below 30bar), after removing a large amount of ethanol and water, the The concentrate was left to stand for more than 3 hours, and the supernatant was taken, and spray-dried with a spray dryer with a product cooling collection system (inlet air temperature 150° C.) to obtain 31.6 kg of powder. As detected by HPLC, the NMN content was 3.5%.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a preparation method of a lentinus edodes extract containing NMN. The preparation method comprises the following steps of adding an ethanol solution into fresh lentinus edodes, and carrying out ultrasonic treatment and soaking to obtain a leaching solution; filtering the leaching solution, then carrying out molecular distillation, and removing ethanol and water to obtain a concentrated solution; and standing the concentrated solution, taking supernate, and drying to obtain the lentinus edodes extract containing the NMN. According to the method, an extract containing NMN is extracted from fresh lentinus edodes. The content of the NMN in the obtained extract is greater than 3%, and the yield of the NMN is greater than five per ten thousand.

Description

technical field [0001] The present invention relates to the field of preparation of NMN, particularly a kind of preparation method of the mushroom extract containing NMN. Background technique [0002] NMN (nicotinamide mononucleotide) is the precursor of intracellular NAD+ (nicotinamide adenine dinucleotide), NAD+ is the redox coenzyme of the tricarboxylic acid cycle, NMN is an intermediate in the NAD+ salvage pathway, and has anti- Oxidation and reducing oxidative stress can enhance energy metabolism, slow down the physiological decline of organisms, and prolong life span. Harvard Medical School, Washington University, Japan's Kei University and other top scientific research institutions in the world have respectively confirmed its effectiveness from multiple perspectives, including reversing muscle atrophy to improve physical fitness, inhibiting cognitive decline caused by aging, reversing vascular death and protecting cardiovascular and cerebrovascular functions. Signifi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A23L2/39A23L2/38A23L33/10A23L31/00A23L33/00
CPCA23L2/39A23L2/38A23L33/10A23L31/00A23L33/00A23V2002/00A23V2200/30A23V2250/208A23V2300/10A23V2300/48
Inventor 林军陈顺成钱哲锋
Owner 金华贝塔生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com