Method for separating and purifying staphylococcus aureus in proteusbacillus vulgaris

A technology of Staphylococcus and Proteus, applied in the directions of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of inability to separate pure Staphylococcus aureus, etc., to shorten the time of purification, good repeatability, and shorten the effect of time period

Pending Publication Date: 2021-05-11
孙杨
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The object of the present invention is: the method for separating and purifying Staphylococcus aureus in Proteus, to solve the existing defect of being unable to separate pure Staphylococcus aureus from Proteus due to the eve growth characteristics of Proteus

Method used

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  • Method for separating and purifying staphylococcus aureus in proteusbacillus vulgaris
  • Method for separating and purifying staphylococcus aureus in proteusbacillus vulgaris
  • Method for separating and purifying staphylococcus aureus in proteusbacillus vulgaris

Examples

Experimental program
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Effect test

Embodiment 1

[0029] The method for separating and purifying Staphylococcus aureus in Proteus comprises the following steps:

[0030] Step 1. Take 2ml of normal saline with a concentration of 0.45% in a drug-sensitive turbidimetric tube;

[0031] Step 2. Put more than 2 aztreonam drug-sensitive paper sheets into the normal saline, mix well to obtain aztreonam drug liquid, and mix with a mixer for 30 seconds;

[0032] Step 3. Dip the aztreonam drug liquid with a cotton swab, and wipe off the excess aztreonam drug liquid;

[0033] Step 4. Dip aztreonam drug liquid with a cotton swab and apply it evenly on the blood agar plate. Repeat 3 times. After each application, turn the blood agar plate at an angle of 60 degrees and let it stand for 8 minutes to obtain aztreonam. Blood agar plates for drugs;

[0034] Step 5, pick a mixture of Proteus and Staphylococcus aureus, inoculate it on a blood agar plate containing aztreonam, and then draw 4 lines;

[0035] Step 6, incubating in the incubator f...

Embodiment 2

[0037] The method for separating and purifying Staphylococcus aureus in Proteus comprises the following steps:

[0038] Step 1. Take 2ml of normal saline with a concentration of 0.45% in a drug-sensitive turbidimetric tube;

[0039] Step 2. Put more than 4 aztreonam drug-sensitive paper sheets into the normal saline, mix well to obtain aztreonam drug liquid, and mix with a mixer for 30 seconds;

[0040] Step 3. Dip the aztreonam drug liquid with a cotton swab, and wipe off the excess aztreonam drug liquid;

[0041] Step 4. Dip the aztreonam drug liquid with a cotton swab and apply it evenly on the blood agar plate. Repeat 2 times. After each application, turn the blood agar plate at an angle of 60 degrees and let it stand for 10 minutes to obtain aztreonam. Blood agar plates for drugs;

[0042] Step 5, pick a mixture of Proteus and Staphylococcus aureus, inoculate it on a blood agar plate containing aztreonam, and then draw 3 lines;

[0043] Step 6, incubating in the incuba...

Embodiment 3

[0045] The method for separating and purifying Staphylococcus aureus in Proteus comprises the following steps:

[0046] Step 1. Take 2ml of 0.9% normal saline in a drug-sensitive turbidimetric tube;

[0047] Step 2. Put more than one piece of aztreonam drug-sensitive paper into the normal saline, mix well to obtain aztreonam drug liquid, and mix with a mixer for 30 seconds;

[0048] Step 3. Dip the aztreonam drug liquid with a cotton swab, and wipe off the excess aztreonam drug liquid;

[0049] Step 4. Dip aztreonam drug liquid with a cotton swab and apply it evenly on the blood agar plate. Repeat 3 times. After each application, turn the blood agar plate at an angle of 60 degrees and let it stand for 6 minutes to obtain aztreonam. Blood agar plates for drugs;

[0050] Step 5, pick a mixture of Proteus and Staphylococcus aureus, inoculate it on a blood agar plate containing aztreonam, and then draw 4 lines;

[0051] Step 6, incubating in the incubator for 16 hours, the Stap...

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Abstract

The invention discloses a method for separating and purifying staphylococcus aureus in proteusbacillus vulgaris, belongs to a microbial separation and purification technology, particularly relates to a staphylococcus aureus separation and purification technology, and aims to overcome the defect that the staphylococcus aureus cannot be separated and purified from the proteusbacillus vulgaris. The method comprises the following steps of taking normal saline; putting an aztreonam drug sensitive paper sheet into the normal saline, and performing uniform mixing to obtain aztreonam drug liquid; dipping the aztreonam drug liquid with a cotton swab; uniformly smearing the aztreonam drug liquid on a blood agar plate, and performing standing for 5-10 minutes to obtain an aztreonam drug-containing blood agar plate; selecting a mixture of the proteusbacillus vulgaris and the staphylococcus aureus, and performing inoculating; and performing incubating in an incubator for 16-24 hours to separate out the staphylococcus aureus. As long as the staphylococcus aureus exists in the mixture, a large number of single pure colonies can be accurately separated out, are not polluted by the proteusbacillus vulgaris and can be directly used for a drug sensitivity test, so that the time period is greatly shortened.

Description

technical field [0001] The invention relates to a method for separating and purifying Staphylococcus aureus from Proteus, and the invention belongs to the technical field of microorganism separation and purification, in particular to the technical field of separation and purification of Staphylococcus aureus. Background technique [0002] Staphylococcus aureus (Staphylococcus aureus), also known as "Staphylococcus aureus", belongs to the genus Staphylococcus and is a common food-borne pathogenic microorganism. Lambert staining is positive, Staphylococcus aureus does not require high nutrition, grows well on common medium, aerobic or facultative anaerobic, optimum growth temperature is 37°C, optimum growth pH is 7.4, the colonies on the plate are thick and shiny , round protrusions, 1-2mm in diameter, and a transparent hemolytic ring formed around the blood plate colony. Staphylococcus aureus has a high degree of salt tolerance, can grow in 10-15% NaCl broth, and can decompos...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/02C12R1/445
CPCC12N1/02C12N1/20
Inventor 孙杨叶梅毅方梦莹
Owner 孙杨
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