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Haplotype molecular markers related to high fecundity of sheep, screening method and application

A technology of molecular markers and haplotypes, applied in the field of molecular genetics, can solve the problems of high fecundity and low fecundity of sheep, etc.

Active Publication Date: 2021-05-11
XINJIANG ACADEMY OF AGRI & RECLAMATION SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the research on MTNR1A gene in sheep mainly focuses on seasonal estrus, and there are few related studies on high fecundity of sheep, and there are no molecular markers and haplotypes of MTNR1A gene applied in molecular breeding of multiparous sheep.

Method used

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  • Haplotype molecular markers related to high fecundity of sheep, screening method and application
  • Haplotype molecular markers related to high fecundity of sheep, screening method and application
  • Haplotype molecular markers related to high fecundity of sheep, screening method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Example 1 Detection and analysis of SNP in the coding region of sheep MTNR1A gene

[0054] 1. Extraction of sheep genomic DNA

[0055] Method 1: Using a general sheep blood genomic DNA extraction method

[0056] (1) Transfer 1 mL of anticoagulated whole blood into a sterile 2 mL centrifuge tube.

[0057] (2) Add an equal volume (1 mL) of PBS buffer, shake gently for 10 min; centrifuge at 3500 g at room temperature for 10 min, discard the supernatant with a pipette, and repeat the steps until the supernatant is transparent and the precipitate is colorless.

[0058] (3) Add 1 mL of DNA extraction solution (10 mmol / L Tris (pH8.0), 0.1 mol / L EDTA, 0.5% SDS) to the centrifuge tube, gently shake to suspend the cell pellet; 37 ° C water bath for 1 h.

[0059] (4) Add 3 μL of proteinase K (final concentration: 60 μg / mL), mix well; incubate in a constant temperature water bath at 55°C (about 2 to 4 hours), until the cell sediment is completely digested and the solution is clar...

Embodiment 2

[0101] Example 2 Detection of Polymorphism Distribution of Molecular Markers Prepared by the Present Invention in Chinese Merino Sheep (Xinjiang Military Reclamation Type) and Multiparous Suffolk Sheep

[0102] The polymorphisms of the three SNP sites in the coding region of the sheep MTNR1A gene were detected, and three genotypes were detected at each site. The genotype frequencies and allele frequencies are shown in Table 3.

[0103] Table 3 Genotype frequency and allele frequency of MTNR1A gene

[0104]

[0105]

[0106] Note: The number in parentheses in the column of genotype frequency is the number of individuals of the genotype.

[0107] It can be seen from Table 3 that three genotypes were detected at the three mutation sites of the sequence. At the MTNR1A_SNP1, MTNR1A_SNP2 and MTNR1A_SNP3 sites, the dominant alleles were G, G, and C, respectively, and the dominant genotypes were GG , GG and CC.

Embodiment 3

[0108] Example 3 Association analysis and application of molecular markers of the present invention and sheep reproductive traits

[0109] In order to determine whether the detected sheep MTNR1A_SNP1, MTNR1A_SNP2 and MTNR1A_SNP3 markers are related to sheep reproductive traits, 224 Chinese Merino sheep (Xinjiang Army Reclamation type) and 190 multiparous Suffolk sheep were selected as experimental materials, and the samples were all from Xinjiang Academy of Agricultural Reclamation Sciences In the breeding sheep farm, the number of lambs of each sheep was recorded, the polymorphism was detected by PCR-RFLP method, and the correlation between different genotypes of the sheep MTNR1A gene coding region and sheep lambing traits was analyzed. The correlation analysis between genotype and phenotype was carried out by using SPSS19.0 software, and the statistical model of genetic effect of genotype was constructed as follows: Y=μ+G+B+(G×B)+e, where Y is the observed value of traits; μ...

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Abstract

The invention discloses a haplotype molecular markers related to high fecundity of sheep, a screening method and application, and belongs to the technical field of molecular genetics. The haplotype molecular markers are composed of an SNP1, an SNP2 and an SNP3; the SNP1 is located at the 735bp position of the MTNR1A gene, and the basic group at the position is G or A; the SNP2 is located at the 753bp position of the MTNR1A gene, and the basic group at the position is G or A; and the SNP3 is located at the 845bp position of the MTNR1A gene, and the basic group at the position is C or A. When the tested sheep SNP1 base is G, SNP2 base is G, SNP3 base is C, and they are all homozygous, or when a haplotype is GGC, the sheep to be detected is a high-fecundity individual. The invention provides an effective and accurate molecular breeding marking method for sheep breeding, which can improve the accuracy and breeding process of group selection, and can play a role in molecular breeding of multifetal mutton sheep and multifetal fine-wool sheep.

Description

technical field [0001] The invention relates to the technical field of molecular genetics, in particular to haplotype molecular markers, screening methods and applications related to high fecundity of sheep. Background technique [0002] In recent years, with the gradual improvement of people's living standards, people have paid more and more attention to a healthy and reasonable diet structure. The demand for mutton with high protein content and low fat and cholesterol content has increased year by year. Breeding sheep with high fecundity, raising sheep with multiple births, and increasing the reproductive rate of sheep groups can significantly increase the number of lambs on hand, and alleviate the situation of high mutton prices and tight supply in the market. With the implementation of various policies and measures of closing mountains and prohibiting grazing, sheep breeding has changed from traditional grazing and free-range feeding to house feeding. Power sheep has be...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156Y02P60/87
Inventor 杨华杨永林王明远赵宗胜余乾张宾倪建宏陈宁卢守亮刘长彬
Owner XINJIANG ACADEMY OF AGRI & RECLAMATION SCI
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