Preparation method of 2-hexenal absorption developing film
A technology of hexenal and color film, which is applied in material analysis, measuring device, instrument and other directions by observing the influence on chemical indicator, which can solve the problems of complicated and cumbersome analysis process.
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Embodiment 1
[0028] (1) 5.321 g polyvinyl alcohol (PVA1799 99%, M W =74885) was dissolved in 40 mL of deionized water, cooled to 50 °C, added with 10 ml of absolute ethanol, and stirred for 2 hours under constant temperature magnetic force to dissolve to obtain a casting solution. Use a 10mL syringe to draw 5mL onto a dry and clean Petri dish, and put it into a 45°C oven to dry it into a PVA film.
[0029] (2) Accurately weigh 0.0175g of methyl red sodium salt into a mixture of 25ml of water and 21ml of methanol, stir for 1 hour, then slowly add 4ml of glycerin, and stir evenly with magnetic force at room temperature to obtain an aldehyde indicator. Use a pipette gun to take 1.6ml of aldehyde indicator and drop it on the PVA film and dry it to obtain a chromogenic film. The dosage of the aldehyde indicator is to drop 0.08ml per square centimeter of film.
[0030] (3) Suspend the obtained chromogenic film to contain 50 μL of 89.47 mg / mL 2-hexenal solution and control the sensing distance ...
Embodiment 2
[0032] This example is used to illustrate the drawing of the standard curve for the detection of 2-hexenal by the colorimetric film.
[0033] Prepare a standard solution to be tested containing 2-hexenal in a concentration gradient, wherein the concentration of 2-hexenal is 20, 40, 60, 80, 100, 120, 140, 160, 180, 200 mg / ml. The chromogenic film of Example 1 was suspended until it contained 50 μL of the above-mentioned standard solution to be tested, and the sensing distance was controlled to be 0.7 cm, and the color was developed by adsorption at an ambient temperature of 65 °C for 60 min.
[0034] Use image J software to analyze the color of 2-hexenal before and after the response, and the specific steps are as follows: select the same measurement area that can include all the color-developing areas, and sequentially measure the color of the culture dish covered with the color-developing film before and after adsorption. The color area is the average gray value of each color...
Embodiment 3
[0036] (1) Dissolve 6.315 g of polyvinyl alcohol (PVA1799) in 45 mL of deionized water at 90 °C, cool down to 50 °C, add 15 ml of absolute ethanol, and stir for 2 h at constant temperature to obtain a casting solution. Use a 10mL syringe to draw 5mL onto a dry and clean Petri dish, and put it into a 45°C oven to dry it into a PVA film.
[0037] (2) Accurately weigh 0.0175g of methyl red sodium salt into a mixture of 25ml of water and 21ml of methanol, stir for 1 hour, then slowly add 4ml of glycerin, and stir evenly with magnetic force at room temperature to obtain an aldehyde indicator. Use a pipette gun to take 1.6ml of aldehyde indicator and drop it on the PVA film and dry it to obtain a chromogenic film. The dosage of the aldehyde indicator is to drop 0.08ml per square centimeter of film.
[0038](3) Suspend the obtained chromogenic film to contain 50 μL of 180 mg / mL 2-hexenal solution and control the sensing distance to 0.7 cm, and absorb and develop color at an ambient ...
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