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Mutant strain of trichoderma reesei, and protein manufacturing method

A technology of Trichoderma reesei and mutant strains, applied in botany equipment and methods, microorganism-based methods, biochemical equipment and methods, etc., can solve the problems of increased oxygen supply, decreased oxygen volume transfer coefficient, and large shear damage and other problems, to achieve the effect of increasing the production volume and suppressing the decrease of dissolved oxygen saturation

Inactive Publication Date: 2021-05-14
TORAY IND INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, if the size of the culture tank is increased, the oxygen volume transfer coefficient will decrease. Therefore, in order to maintain the dissolved oxygen saturation in the culture at a certain level or more, it is necessary to further increase the number of stirring and the amount of oxygen supply.
However, if the number of stirring is increased, there is a problem that a large shear damage is imparted to the bacterial cells, and there is also a problem that a large amount of energy is required to increase the oxygen supply amount.

Method used

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  • Mutant strain of trichoderma reesei, and protein manufacturing method
  • Mutant strain of trichoderma reesei, and protein manufacturing method
  • Mutant strain of trichoderma reesei, and protein manufacturing method

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Embodiment

[0147] The following examples will be given to specifically describe the present invention.

reference example 1

[0148] Protein Concentration Measurement Conditions

[0149] Protein concentration measurement reagent used: Quick Start Bradford Protein Measurement Kit (manufactured by Bio-Rad)

[0150] Measurement conditions

[0151] Measurement temperature: room temperature

[0152] Protein concentration determination reagent: 250 μL

[0153] Culture solution of filamentous bacteria: 5 μL

[0154] Response time: 5 minutes

[0155] Absorbance: 595nm

[0156] Standard: BSA.

[0157] Calculation of Dissolved Oxygen Saturation

[0158] Dissolved oxygen saturation refers to the ratio of dissolved oxygen during the culture period to saturation when the culture conditions are set to pH and temperature using a bacteria-free medium, and the saturation state of dissolved oxygen when the air is ventilated is taken as 100%. The proportion of oxygen is calculated as dissolved oxygen saturation. As the DO meter, a sealed dissolved oxygen electrode SDOC-12F-L120 (manufactured by Eibul Corporatio...

reference example 4

[0161] Determination of the amount of bacteria

[0162] In order to measure the amount of bacterial cells contained in the culture solution, the culture solution was suction-filtered with filter paper, and the difference in the dry bacterial cell weights of the filter paper before and after suction filtration was used as the bacterial cell amount.

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Abstract

The present invention relates to a mutant strain of Trichoderma reesei that has a mutation eliminating or reducing a function of a polypeptide that comprises the amino acid sequence shown in SEQ ID NO:8, said mutant strain preferably further having the mutation indicated hereinafter in one or more polypeptides selected from polypeptides comprising the amino acid sequence shown in any of SEQ ID NO:6, 7, 9, and 10. Proteins, especially cellulases, can be produced using said mutant strain, while keeping the viscosity of a cultivation liquid low during cultivation.

Description

technical field [0001] The present invention relates to a mutant strain of Trichoderma reesei capable of maintaining low viscosity of a culture solution and improving protein production ability, and a method for producing a protein using the mutant strain. Background technique [0002] Trichoderma reesei is known to have a high ability to produce proteins, and studies have been conducted on protein production using Trichoderma reesei. Trichoderma reesei uses cellulose, lactose, cellobiose, and the like as inducers to produce cellulase, which is classified as a saccharification enzyme, among proteins. In order to enhance the amount of cellulase production, many studies have been conducted since ancient times on gene changes represented by overexpression and deletion of factors controlling cellulase production, optimization of culture conditions, and the like. [0003] On the other hand, Trichoderma is an aerobic filamentous fungus that requires oxygen for growth and reproduc...

Claims

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Application Information

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IPC IPC(8): C12N1/15C12N15/31C12P21/02
CPCC12P21/02C12N15/80C12R2001/885C07K14/37C12N9/2437
Inventor 加川雄介平松绅吾山田胜成
Owner TORAY IND INC
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