Biologically active peptide with amino acid structure vvrkplnkegkkp and its preparation method and application
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A bioactive peptide and amino acid technology, applied in the protein field, to achieve the effect of exerting the body's immune function, improving the ability, and good anti-inflammatory activity
Active Publication Date: 2022-04-08
ZHEJIANG HUITAI LIFE HEALTH TECH CO LTD
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At present, there is no research on the function of the polypeptide fragment of 40S ribosomal protein S6 protein in the prior art
Method used
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Embodiment 1
[0034] The artificial synthesis of embodiment 1 active peptide VVRKPLNKEGKKP and VVRKPLNKEGKKPR
[0035] 1. Synthesis of Bioactive Peptides
[0036] 1. Weigh 3g of RINK resin (degree of substitution: 0.3mmol / g) into a 150ml reactor and soak with 50ml of dichloromethane (DCM).
[0037] 2. After 2 hours, wash the resin with nitrogen-dimethylformamide (DMF) that is 3 times the volume of the resin, and then drain it. Repeat this four times, and drain the resin for use.
[0038] 3. Add a certain amount of 20% piperidine (piperidine / DMF=1:4, v:v) into the reactor, and shake it on a decolorizing shaker for 20 minutes to remove the Fmoc protecting group on the resin. After deprotection, wash four times with 3 times the resin volume of DMF, and then drain.
[0039] 4. Take a small amount of resin and test it with ninhydrin (Ninhydrin Nine Wells) method (two drops each of test A and test B, react at 100°C for 1 min), if the resin has color, it means that the deprotection is successful...
Embodiment 2
[0078] Example 2 The immunomodulatory activity experiment of biologically active peptides
[0079] 1. Determination of the amount of bioactive peptide VVRKPLNKEGKKP that promotes the induction of nitric oxide in macrophages (Griess method)
[0080] 1. Experimental reagents and instruments:
[0081]Reagents: experimental animal balb / c mice (male 6-8 weeks old) spleen lymphocyte-derived bioactive peptide VVRKPLNKEGKKP; LPS, purchased from Sigma Company; neutral red staining solution, produced by Beyond Biotechnology Research Institute.
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Abstract
The present invention relates to the field of protein, in particular to a biologically active peptide having an amino acid structure VVRKPLNKEGKKP and its preparation method and application. The amino acid sequence of the biologically active peptide VVRKPLNKEGKKP is shown in SEQ ID NO: 1, and the amino acid sequence of the biologically active peptide VVRKPLNKEGKKPR is shown in SEQ ID NO: 2 shown. In vitro immunoregulatory function experiments have proved that the bioactive peptides VVRKPLNKEGKKP and VVRKPLNKEGKKPR have good immune regulatory functions. The VVRKPLNKEGKKP and VVRKPLNKEGKKPR of the present invention have the ability to promote the increase in the induction of macrophage nitric oxide under certain concentration conditions, thereby improving the body's ability to resist external pathogen infection, reducing the incidence of the body, and at the same time promoting the proliferation of macrophages in vitro and exerting their full potential. The immune function of the body is of great significance to the development of food, health products and medicines with immune regulation function.
Description
technical field [0001] The invention relates to the field of proteins, in particular to a biologically active peptide with an amino acid structure VVRKPLNKEGKKP and a preparation method and application thereof. Background technique [0002] Because of its many potential biological functions, bioactive peptides have attracted more and more attention and become one of the hotspots of scientific research. At present, the beneficial effects of many bioactive peptides have also been well proven, such as anti-cancer, lowering blood pressure, antibacterial, lowering cholesterol, anti-diabetic and other properties. More than 3,000 different bioactive peptides have been reported in BIOPEP-UMW, the most authoritative bioactive peptide database. However, due to the large number of living peptides, there are still many peptides to be studied for their related properties. [0003] At present, most studies on bioactive peptides focus on food-derived peptides, while there are few studies ...
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