Method for detecting expected curative effect of docetaxel and cis-platinum and efficient typing of gene loci related to toxic and negative effects and application of gene loci efficient typing method

Abstract

The invention discloses a method for detecting expected curative effects of docetaxel and cis-platinum and efficient typing of gene loci related to toxic and negative effects and application of the method. The efficient typing method comprises the following steps of constructing a detection site table, determining a to-be-detected SNP site, synthesizing an amplification primer, preparing a solvent, carrying out an amplification reaction, carrying out an SAP reaction, carrying out a single base extension reaction, desalting and purifying, and analyzing data to obtain a result. According to the method, expected curative effects of docetaxel and cis-platinum and gene loci related to toxic and negative effects can be rapidly and accurately detected, the operation process is simple, the detection accuracy is high, the detection flux is high, and the detection cost is low; data are judged and read through an instrument, the result analysis is convenient, the interpretation standard is clear, and the consumed time is short; and the operation is convenient, and market practical applicability is achieved.

Description

technical field [0001] The invention relates to a high-efficiency typing method for detecting gene loci related to the expected curative effect and toxic side effects of docetaxel and cisplatin and its application, belonging to the field of biotechnology. Background technique [0002] With the rapid development of the global life science field, drug efficacy and toxic side effects have become hot issues in the medical field. Due to the adverse reactions of drugs, the treatment of patients brings huge hidden dangers. [0003] In recent years, a large number of clinical studies have shown that each chemotherapy drug has a corresponding target to evaluate its effect, such as CASP7, ABCC2, TP53, ERCC1 and other genes. The expression levels and polymorphisms of these genes can scientifically predict the expected curative effect and toxic side effects of docetaxel and cisplatin, improve the pertinence of treatment, and provide guidance for clinical medication. [0004] Docetaxel...

AI Technical Summary

Problems solved by technology

[0006] Existing gene detection technologies mainly include fluorescent PCR method, fluorescent chip method, Sanger sequencing, next-generation sequencing, etc., all of which have certain defects; the flux of the fluorescent PCR method is low and the cost is high; the operation of Sanger sequencing is cumbersome and the throughput Low; the cost of next-generation sequencing is very high

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