Dissolved sugar barrier test strip as well as preparation method and use method thereof

A technology for test strips and dissolving sugar, which is applied in the direction of biochemical equipment and methods, microbe measurement/inspection, etc., can solve the problems of slowing down, etc., and achieve the effects of simple preparation, prolonging reaction time, and increasing reaction time

Pending Publication Date: 2021-05-25
SHAANXI UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is to provide a kind of dissolving sugar barrier test strip and preparation method thereof and using method thereof, this method solves existing by slowing down flow velocity and strengthens test paper sensitivity method and only focuses on one aspect of research (marked nano-gold and The reaction time of the target, or the reaction time of the complex and the capture object after the labeled gold nanoparticles are combined with the target)

Method used

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  • Dissolved sugar barrier test strip as well as preparation method and use method thereof
  • Dissolved sugar barrier test strip as well as preparation method and use method thereof
  • Dissolved sugar barrier test strip as well as preparation method and use method thereof

Examples

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Effect test

preparation example Construction

[0019] The method for preparing and using the dissolved sugar barrier test strip of the present invention, the specific steps are as follows:

[0020] Step 1: prepare sucrose solution;

[0021] Step 2: Prepare blank test strips;

[0022] Step 3: Add quality control line probes, detection line probes and gold nanoparticles labeled with detection probes on the surface of the nitrocellulose membrane of the blank test strip prepared in step 2 to prepare conventional test strips;

[0023] Step 4: Add sucrose solution, quality control line probe, detection line probe and gold nanoparticles labeled with detection probe to the blank test strip prepared in step 2 to prepare a test strip with a sugar-dissolving barrier;

[0024] Step 5: Detect the conventional test strip and the dissolved sugar barrier test strip prepared in steps 3 and 4, and compare the difference in sensitivity.

[0025] The specific process of step 1 is: take 80g~100g sucrose and dissolve in 100ml of ultrapure wat...

Embodiment 1

[0030] Example 1: nucleic acid detection

[0031] A method for dissolving the sugar barrier to enhance the detection sensitivity of the test paper, comprising the following steps:

[0032] Step 1, prepare sucrose solution;

[0033] Weigh 80g of sucrose and dissolve it in 100mL of ultrapure aqueous solution to prepare a saturated sucrose solution, dilute the saturated solution to a sucrose solution with a mass concentration of 10%, and store it at room temperature for subsequent use.

[0034] Step 2, preparing blank test strips;

[0035] Paste the absorbent paper, nitrocellulose membrane, binding pad, and sample pad on the back-adhesive board in sequence (any two of them overlap each other by 2mm), and then use a cutting machine to cut it into 3mm-wide blank test paper.

[0036] Step 3, add quality control line probes, detection line probes and gold nanoparticles labeled with detection probes on the surface of the blank test strip nitrocellulose membrane prepared in step 2 to...

Embodiment 2

[0044] Embodiment 2: Nucleic acid detection

[0045] A method for dissolving the sugar barrier to enhance the detection sensitivity of the test paper, comprising the following steps:

[0046] Step 1: prepare sucrose solution;

[0047] Weigh 80g of sucrose and dissolve it in 100mL of ultrapure aqueous solution to prepare a saturated sucrose solution, dilute the saturated solution to a sucrose solution with a mass concentration of 10%, and store it at room temperature for subsequent use.

[0048] Step 2: Prepare blank test strips;

[0049] Paste the absorbent paper, nitrocellulose membrane, binding pad, and sample pad on the back-adhesive board in order (any two of them overlap each other by 2mm), and then cut it into 3mm-wide blank test strips with a cutting machine.

[0050] Step 3: Add quality control line probes, detection line probes and gold nanoparticles labeled with detection probes on the surface of the blank test strip nitrocellulose membrane prepared in step 2 to pr...

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Abstract

The invention provides a dissolved sugar barrier test strip and a preparation method and a use method thereof. The test strip sequentially comprises absorbent paper, a nitrocellulose membrane, a combination pad and a sample pad from one end to the other end, and a quality control line formed by a quality control line probe and a detection line formed by a detection line probe are arranged on the surface of the nitrocellulose membrane; nanogold marked by a detection probe is arranged on the combination pad; and sucrose barriers are arranged on the surface of the nitrocellulose membrane between the quality control line and the detection line and the surface of the nitrocellulose membrane between the detection line and the combination pad. Through the redissolution process of sugar, the flowing speed of liquid to be detected is slowed down, the reaction time between a target object and marked nanogold and the reaction time between a target object and nanogold combined compound and captured molecules in the detection process are prolonged, and the detection sensitivity of the test strip is enhanced.

Description

technical field [0001] The invention belongs to the field of rapid test paper detection of various pathogens, and relates to a test paper strip for dissolving sugar barrier and a preparation method and a use method thereof. Background technique [0002] As a real-time detection technology, lateral flow chromatography test paper has been widely used in the detection fields of disease diagnosis, environmental monitoring and food safety. It can realize the detection of different targets, such as: early pregnancy test paper, blood sugar test paper, HIV test paper, Helicobacter pylori test paper, nitrite detection test paper, heavy metal lead and mercury detection test paper, etc. However, the low detection sensitivity of lateral flow chromatography test paper limits its further development. [0003] So far, many scholars have improved the detection sensitivity of lateral flow chromatography test paper by slowing down the flow rate in different ways. For example: wax barrier ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6834
CPCC12Q1/6834C12Q2565/625C12Q2565/519C12Q2563/137C12Q2563/155
Inventor 唐蕊华谢明月李敏倪永浩
Owner SHAANXI UNIV OF SCI & TECH
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