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Light sheet fluorescence microscopic imaging method and device based on double-core optical fiber light control

A dual-core optical fiber and microscopic imaging technology, which is applied in microscopes, measuring devices, fluorescence/phosphorescence, etc., can solve problems such as expensive scanning mirrors, complex structures of light sheet microscopes, and poor cell movement accuracy.

Active Publication Date: 2021-05-25
GUILIN UNIV OF ELECTRONIC TECH
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Problems solved by technology

Scanning mirrors are expensive, and the addition of scanning mirrors will complicate the structure of the light sheet microscope
Using a micron-level micro-stage, the accuracy of cell movement cannot meet the requirements. Although the precision of a nano-level micro-stage is high enough, the high-precision micro-stage is expensive

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  • Light sheet fluorescence microscopic imaging method and device based on double-core optical fiber light control
  • Light sheet fluorescence microscopic imaging method and device based on double-core optical fiber light control
  • Light sheet fluorescence microscopic imaging method and device based on double-core optical fiber light control

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Embodiment Construction

[0025] The present invention will be further described in detail below in conjunction with examples, so that those skilled in the art can implement it with reference to the description.

[0026] It should be understood that terms such as "having", "comprising" and "including" used herein do not exclude the presence or addition of one or more other elements or combinations thereof.

[0027] A sheet light imaging method and device based on dual-core optical fiber light manipulation. It is characterized in that: it consists of laser light source 1, 10, 31; lens 2, 3, 13, 14, 25, 28; cylindrical lens 4; apochromatic microscopic objective lens 5, 9; CMOS camera 6; imaging lens 7; Optical sheet 8; single-mode optical fibers 15 and 16, 23, 24, fiber couplers 17, 22; 5 / 5 beam splitters 12, 27; gradient attenuation sheets 26, 29; mirrors 12, 30 and cells to be tested 19 . The laser light source 1 emits laser light, expands the beam through the lenses 2 and 3, passes through the cylin...

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Abstract

The invention provides a light sheet fluorescence microscopic imaging method and system based on double-core optical fiber light control. The system is characterized in that a light control part of the device is formed by oppositely installing two two-core optical fibers of which the output end surfaces are processed into specific angles, wherein a focused light field formed near the output end face of one two-core optical fiber stably captures a cell to be detected and adjusts the output power of each fiber core of the other two-core optical fiber, so that the cell rotates around a specific axis. And when the cells rotate to a certain angle and reach a stable state every time, a structure image of a certain layer of cells is obtained by using a light sheet fluorescence microscopy technology, and finally a three-dimensional structure image of the cells is obtained. The system constructed by the invention can be used for obtaining high-time and high-spatial resolution three-dimensional structure images of living single cells, and has the characteristics of small light damage, simple structure, low cost, simplicity and convenience in operation and the like. The system can be applied to many research fields such as biology, medicine and life science.

Description

[0001] (1) Technical field [0002] The invention provides a sheet light imaging method and device based on dual-core optical fiber control, which can be used to control the rotation of living single cells through optical fibers to achieve tomographic imaging of cells, and belongs to the field of optical microscopic imaging. [0003] (2) Background technology [0004] Cells are the basic unit of life structure and function. In-depth research on cells is the key to revealing the mysteries of life phenomena, transforming life and conquering diseases. There are huge individual differences in enzyme activity, gene expression, and cell signal transduction among individual cells, even among individual cells cultured in vitro with the same genetic background. Analytical studies of single cells can reveal phenomena and mechanisms not observed when studying large populations of cells. At present, one of the hot spots in life science research is to clarify the basic activity rules of si...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N15/14G01N21/64G02B21/00
CPCG01N21/6458G01N15/1434G01N15/1468G02B21/0032G02B21/0076G01N2015/1445
Inventor 尹君陈宏宇于凌尧王少飞贾源胡徐锦苑立波
Owner GUILIN UNIV OF ELECTRONIC TECH
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