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Method for rapidly determining content of components in corncob hydrolysate

A technology for corncob hydrolyzate and component content, which is applied in measurement devices, material separation, and analysis of materials, etc., can solve the problem that sugar and inhibitor components cannot be quantitatively detected at the same time, and achieve reliable measurement results and short analysis time. , the effect of high detection efficiency

Inactive Publication Date: 2021-05-25
ZHEJIANG HUAKANG PHARMA
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The technical problem to be solved by the present invention is to provide a method for rapidly measuring the content of components in the corncob hydrolyzate, which is simple and easy Easy, fast, convenient and stable, can simultaneously separate and detect the concentration of monosaccharides and monosaccharides, as well as between monosaccharides and acetic acid and furfural

Method used

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  • Method for rapidly determining content of components in corncob hydrolysate
  • Method for rapidly determining content of components in corncob hydrolysate
  • Method for rapidly determining content of components in corncob hydrolysate

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Embodiment 1

[0049]A preferred embodiment of a method of rapidly determining component content in corn core hydrolyzing fluids, including the following steps:

[0050]1), the preparation of corn core hydrolyzing:

[0051]First, the corn core feedstock was pulverized with a pulverizer, and then the molecular sieve was sieved to obtain corn core particles of 35 mesh to 80 mesh diameter, and after drying at 105 ° C for 3 h, it was taken out and stored in a brown fine glass bottle.

[0052]Use electronic analysis to weigh 2.00g (no quality) corn core particles are placed in a blue cover water solution, add a certain quality ultrapure water (controlled solid-liquid ratio within a certain range, 1: 5 ~ 1: 15) And 1.0% (wt%) of the dilute sulfuric acid solution, then placed the hydrolysis bottle to perform the rapid uniform mixing of the liquid liquid, then transferred to the vertical pressure steam sterilizer, warm to 121 ° C temperature hydrolysis Remove the cooling after 60min. Finally, the acid hydrolyzed l...

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Abstract

The invention relates to a method for rapidly determining the content of components in corncob hydrolysate. Five components, namely glucose, xylose, arabinose, acetic acid and furfural, in a corncob hydrolysate sample to be determined are simultaneously subjected to content determination and analysis by HPLC (High Performance Liquid Chromatography) analysis, the component analysis in the corncob hydrolysate is completed at one time, the peak patterns of the substances are sharp, and the separation effect is obvious; the analysis is completed within 15 minutes, and the analysis time is short; the used equipment is common, the mobile phase is simple to prepare, the method is easy to implement, and the detection cost is relatively low. Calculating is performed through a standard solution external standard method to obtain the content of each component in the corncob hydrolysate. The standard curve obtained by the method has the advantages of favorable linear relationship, high precision and accuracy, the measurement result is reliable, the detection efficiency is high, and the method can be operated in batches.

Description

Technical field[0001]The present invention belongs to the field of corn core hydrolyzing component, and in particular, the present invention relates to a method of rapidly determining component content in corn core hydrolyzing.Background technique[0002]The corn core is rich in half cellulose and cellulose, which can be obtained by glycose, glucose, glycose, sugar substances such as xylose, glucose, arabic sugar after acid or enzymatic hydrolysis, and hydrolysis by-products such as furfural and acetic acid. During the hydrolysis process, it is necessary to track the change in the component of the hydrolyzing, and the detection analysis method in general use is high performance liquid chromatography and gas chromatography.[0003]Sugar material analysis is commonly used in high performance liquid chromatography, the detector is mainly based on the variance of the refractive light detector and the evaporation light scatter detector, such as the national standard GB / T23532-2009 "xylose"...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/74
CPCG01N30/02G01N30/06G01N30/74G01N2030/062
Inventor 傅丹宁曾徐浩唐艳军李勉胡昌辉王静
Owner ZHEJIANG HUAKANG PHARMA
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