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BeSO4 contaminated rat lung tissue differential expression protein detection and analysis method

A technology of differentially expressed protein and analysis method, applied in the field of detection and analysis of differentially expressed protein in the lung tissue of rats exposed to BeSO4, can solve the problem of incomplete elucidation of the toxicological mechanism, and achieve strong separation ability, wide adaptability and high sensitivity Effect

Pending Publication Date: 2021-05-28
NANHUA UNIV
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Problems solved by technology

[0004] At present, the toxicological mechanism of beryllium compound-induced lung injury has not been fully elucidated, and the treatment for "beryllium lung" is only symptomatic and supportive.

Method used

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  • BeSO4 contaminated rat lung tissue differential expression protein detection and analysis method
  • BeSO4 contaminated rat lung tissue differential expression protein detection and analysis method
  • BeSO4 contaminated rat lung tissue differential expression protein detection and analysis method

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Embodiment 1

[0063] BeSO 4 The method for detection and analysis of differentially expressed proteins in the lung tissue of poisoned rats, the steps are as follows:

[0064] S01, obtaining rat lung tissue:

[0065] a. Select 10 healthy male rats and randomly divide them into a control group of 5 rats and a poisoning group of 5 rats. They were fed with water and fed for 6 weeks, and the poisoned group was instilled with 0.25 ml of BeSO 4 solution into the rat trachea, and the control group instilled 0.25ml of sterilized ultrapure water into the rat trachea in the second week;

[0066] b. A total of 10 rats in the poisoning group and the control group were anesthetized with ether, and blood was collected by the abdominal aorta blood collection method. After the rats died, the lungs were collected for subsequent use.

[0067] In this step, BeSO 4 The concentration of the solution was 12ml / kg.

[0068] In this step, the breed of rats is SD rats.

[0069] S02, sample preparation:

[0070...

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Abstract

The invention discloses a BeSO4 contaminated rat lung tissue differential expression protein detection and analysis method. The method comprises the following steps: 1, obtaining rat lung tissues; 2, preparing a sample; 3, carrying out protein reduction treatment; 4, performing protein alkylation treatment; 5, carrying out proteolysis; 6, carrying out TMT labeling on the protein; 7, performing LC-MS / MS detection on the sample; 8, carrying out enrichment analysis on GO and KEGG; and 9, carrying out PPI network diagram analysis. According to the invention, by using a TMT-labeled quantitative proteomics technology and a bioinformatics analysis technology, the protein expression condition of the BeSO4 contaminated rat lung tissue is rapidly detected in a high-throughput mode, and key protein is rapidly and accurately screened out of the rat lung tissue, so that the research direction is pointed out for the occurrence mechanism of the BeSO4-induced lung cell injury, and the important guiding significance is achieved for diagnosis and treatment of the beryllium lung.

Description

technical field [0001] The invention relates to the field of experimental research on lung diseases, in particular to a BeSO 4 Detection and analysis methods of differentially expressed proteins in lung tissue of poisoned rats. Background technique [0002] Beryllium (Be) is a gray-white alkaline earth metal with a high melting point and strong electrical and thermal conductivity. It is mainly used in three forms: beryllium metal, beryllium alloy and beryllium oxide, and is widely used in aviation. Aerospace, information technology, medical industry and nuclear industry. [0003] Beryllium was listed as a Class I carcinogen by the International Agency for Research on Cancer as early as 1993. Exposure to beryllium compounds can cause acute beryllium disease, beryllium sensitization, chronic beryllium disease, and lung cancer. Beryllium exposure (that is, exposure to environments containing beryllium compounds) can damage multiple organs throughout the body. The lung is the...

Claims

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Application Information

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IPC IPC(8): G01N33/68
CPCG01N33/6848
Inventor 张朝晖郑凯刘艳萍徐新云蔡颖雷媛娣
Owner NANHUA UNIV
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