Application of bactericidal composition containing cryptolepine and plant essential oil in prevention and treatment of plant pathogenic fungi

A technology of phyllophylline and plant essential oils, which is applied in the field of natural medicinal chemistry, can solve problems such as the unreported use of plant pathogenic fungi in combination, achieve the effects of reducing pesticide costs, reducing dosage, and improving control effects

Pending Publication Date: 2021-06-04
LANZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report about the combined use of basaltine and plant essential oils on phytopathogenic fungi. Based on this, this study uses basaltine as the main agent and plant essential oils as an auxiliary agent in the prevention and treatment of Rhizoctonia solani. , Sclerotinia sclerotiorum, tomato cinerea, wheat head blight, rice blast fungus plant pathogenic fungi

Method used

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  • Application of bactericidal composition containing cryptolepine and plant essential oil in prevention and treatment of plant pathogenic fungi
  • Application of bactericidal composition containing cryptolepine and plant essential oil in prevention and treatment of plant pathogenic fungi
  • Application of bactericidal composition containing cryptolepine and plant essential oil in prevention and treatment of plant pathogenic fungi

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Embodiment 1: a kind of fungicide composition containing phyllophylline and plant essential oil, comprises active component A and active component B (as shown in structural formula 1), and described active component A is phyllophylline, The active component B is plant essential oil.

[0014]

Embodiment 2

[0015] Embodiment 2: Determination of indoor antibacterial activity of phytopathogenic fungi and results

[0016] The phytopathogenic fungi used in this experiment were strains preserved at 4°C in the laboratory, and the mycelial growth method was adopted. All phytopathogenic fungi in the laboratory were grown on potato agar dextrose medium (PDA for short). PDA medium formula: potato (peeled) 200g, glucose 20g, agar 15g, distilled water 1000mL, natural pH.

[0017] Preparation method of PDA medium: Wash and peel the potatoes, weigh 200g and cut into small pieces, add water and boil until rotten (boil for 20-30 minutes, it can be pierced by a glass rod), filter with four layers of gauze and fill the water in a beaker To 1000mL, add 15-20g of agar according to the needs of the experiment, add 20g of glucose, stir to make it fully dissolved, then divide into Erlenmeyer flasks, sterilize at 121°C for 20 minutes, and cool down for later use.

[0018] experimental method

[0019] ...

Embodiment 3

[0029] Example 3: Indoor activity determination of Rhizoctonia solani mixed with basaltine and active component B

[0030] The mixed synergistic effect of fungicides is calculated according to the Wadley (1967) formula to calculate the synergistic effect coefficient after mixing different agents:

[0031]

[0032] In the formula, a and b respectively represent the proportions of phyllophylline (A) and plant essential oil (B) in the mixture, EC 50 (A), EC 50 (B) represent the actual observed EC 50 value, EC 50 (Exp) represents the theoretical EC of two agents A and B mixed according to a:b 50 value, EC 50 (Obs) is the actual observed EC after mixing the two agents of A and B according to a:b 50 value. SR>1.5 is synergistic; SR<0.5 is antagonistic; SR between 0.5 and 1.5 is additive.

[0033] Table 2 shows the indoor virulence synergistic effect of basaltine and active component B on Rhizoctonia solani.

[0034] Table 2 Indoor virulence synergistic effect of basaltine...

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Abstract

The invention belongs to the field of natural medicinal chemistry, and discloses a composition containing cryptolepine and plant essential oil (oregano essential oil, pogostemon cablin essential oil, cinnamon essential oil, litsea cubeba essential oil, clove essential oil, thyme essential oil, geranium essential oil, garlic essential oil, tea tree oil, calamus oil, lemon grass essential oil, ocimum gratissimum oil, zedoary turmeric oil, West Indian sandalwood oil and violet essential oil). The composition is used for preventing and treating diseases caused by rhizoctonia solani, sclerotinia sclerotiorum, fusarium graminearum, botrytis cinerea and magnaporthe oryzae, and the mass part ratio of cryptolepine to plant essential oil is 1:10 or 1:20. The bactericidal composition provided by the invention has the advantages of wide bactericidal spectrum, low toxicity and reduction of drug resistance of plants, and has further research and development values.

Description

technical field [0001] The invention belongs to the field of natural medicinal chemistry, and relates to a bactericidal composition containing two active ingredients of phyllophylline and plant essential oil, and the composition is used for preventing and treating rhizoctonia solani, Sclerotinia sclerotiorum, tomato cinerea, wheat Use in diseases caused by scab, cotton fusarium wilt and rice blast fungus. Background technique [0002] Phytopathogenic fungi refer to those fungi that can parasitize on plants and cause plant diseases. Diseases caused by plant pathogenic fungi account for about 70-80% of plant diseases and insect pests. The agricultural diseases caused by plant pathogenic fungi have the characteristics of many types, great impact and frequent outbreaks. The scope and severity of their occurrence have caused very significant losses to my country's national economy and agricultural production. The use of chemical reagents is still the main means of preventing an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N43/90A01N65/22A01N65/24A01N65/28A01N65/40A01N65/42A01N65/44A01N65/48A01N65/08A01P3/00
CPCA01N43/90A01N65/00A01N65/08A01N65/22A01N65/24A01N65/28A01N65/40A01N65/42A01N65/44A01N65/48
Inventor 刘映前吴天琳杜莎莎张智军贺颖慧胡勇梅马越杨程杰赵文斌
Owner LANZHOU UNIVERSITY
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