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Porcine-derived fibrin-electrostatic spinning nanofiber antibacterial hemostatic patch and preparation method thereof

A technology of electrospinning and fibrin, applied in the direction of cellulose/protein conjugated rayon, rayon manufacturing, fiber chemical characteristics, etc., can solve the problems of complex operation and high storage conditions, and achieve good air permeability and biological Good compatibility, the effect of promoting the formation of granulation tissue

Pending Publication Date: 2021-06-04
SHANGHAI LIKANGRUI BIOLOGICAL ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The new hemostatic patch developed by the present invention overcomes the difficulty in using existing fibrin adhesives such as complex operation, high requirements for storage conditions, and multiple components, and endows it with mechanical properties to promote adhesion and hemostatic effects

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] A pig-derived fibrin-electrospinning nanofiber antibacterial hemostatic patch, the preparation steps are as follows:

[0043] (1) Preparation of antibacterial regenerated silk fibroin electrospinning film: weigh silk fibroin with a mass concentration of 4%, dissolve it in hexafluoroisopropanol, and prepare a silk fibroin solution with a certain mass concentration for subsequent use;

[0044] Weigh polyethylene oxide with a mass concentration of 10%, dissolve it in trifluoroethanol, prepare a polyethylene oxide solution with a mass concentration of 10%, and set aside;

[0045]Mix the prepared silk fibroin solution and polyethylene oxide solution at a volume ratio of 1:2, add nano-silver at a mass concentration of 0.1%, stir magnetically for 2 hours, and let stand to defoam to obtain a spinning solution.

[0046] Fill the spinning solution into a 10mL plastic syringe (a small amount is added in portions, and the remaining liquid continues to vortex), and a 20G blunt stain...

Embodiment 2

[0053] Adjust the amount of each component of the fibrinogen absolute ethanol suspension in step (2) of Example 1 to 40% fibrinogen, 10% sodium citrate, 6% sodium chloride, 11% albumin, 25% Arginine HCl, 4% Riboflavin. The amount of thrombin suspension in absolute ethanol was adjusted to 750 I.U. / mL thrombin, 6% calcium chloride, and 30% mannitol. The rest of the step parameters remain unchanged.

Embodiment 3

[0055] Adjust the amount of each component of the fibrinogen absolute ethanol suspension in step (2) of Example 1 to 35% fibrinogen, 6% sodium citrate, 4% sodium chloride, 15% albumin, 30% Arginine Hydrochloride, 5% Riboflavin. The amount of thrombin suspension in absolute ethanol was adjusted to 1000 I.U. / mL thrombin, 7% calcium chloride, and 40% mannitol. The rest of the step parameters remain unchanged.

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Abstract

The invention relates to the technical field of medical hemostatic patches, and particularly relates to a porcine-derived fibrin-electrostatic spinning nanofiber antibacterial hemostatic patch and a preparation method thereof. The preparation method comprises the following steps of: preparing a porcine-derived fibrinogen and thrombin blended suspension, then spraying or soaking the blended suspension on thes urface of a silk fibroin nanofiber film prepared by an electrostatic spinning technology, and drying to prepare the porcine-derived fibrin hemostatic absorbable patch for postoperative hemostasis. The patch has an excellent hemostatic effect, combines a fibrin thrombin hemostatic mechanism with the mechanical properties of the antibacterial silk fibroin film to enhance the hemostatic effect, and is natural and absorbable, so that the hemostatic effect is improved, and the fibrinogen and thrombin are combined into one, four components are changed into a single component, preparation is not needed, and the patch can be used immediately after being opened, thereby greatly simplifying the problems of complex use and long time consumption of the existing fibrinogen adhesive; and the patch can be stored and transported at room temperature, and has significant technical, cost and clinical advantages.

Description

technical field [0001] The invention relates to the field of medical hemostatic patches, in particular to a nanofiber antibacterial hemostatic patch. Background technique [0002] Bleeding is one of the most common problems in surgery and trauma, and effective hemostatic methods and excellent hemostatic materials are particularly critical. Difficulty in suturing wound bleeding and postoperative suture oozing and other problems often bring troubles to the completion of surgery and postoperative recovery of patients. Choosing a suitable hemostatic dressing can effectively solve this problem. [0003] At present, gelatin sponge and collagen sponge are mostly used as hemostatic auxiliary materials for medical surgery, and the latest hemostatic product, fibrin adhesive, is also used clinically. Medical surgical fibrin adhesive has good hemostasis, tissue compatibility and adhesiveness. By simulating the reaction process of the terminal stage of blood coagulation, it forms a visc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L15/32A61L15/42A61L15/46A61L15/64A61L15/62A61L15/44D01F8/02D01F8/16D01F1/10D04H1/4326D04H1/4382D04H1/728
CPCA61L15/32A61L15/42A61L15/46A61L15/64A61L15/62A61L15/44A61L15/425D01F8/02D01F8/16D01F1/10D01F1/103D04H1/4326D04H1/4382D04H1/728A61L2400/04A61L2300/104A61L2300/404A61L2300/418A61L2300/254A61L2300/252C08L89/00
Inventor 张景添刘璐张军东吴剑英
Owner SHANGHAI LIKANGRUI BIOLOGICAL ENG
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