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BTEX degrading bacterium, screening method therefor and application of BTEX degrading bacterium

A screening method and technology for degrading bacteria, applied in chemical instruments and methods, microorganism-based methods, biochemical equipment and methods, etc., can solve the problems of soil and water environment damage, high cost, and insufficient removal of low-concentration ethylbenzene pollutants. And other issues

Active Publication Date: 2021-06-04
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a kind of BTEX degrading bacteria and its screening method and application, to overcome the destruction of the soil and water body environment caused by the existence of the above-mentioned technology, the removal of low-concentration ethylbenzene pollutants is not thorough enough, requires high cost, and is easy to cause Secondary pollution and other issues

Method used

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  • BTEX degrading bacterium, screening method therefor and application of BTEX degrading bacterium
  • BTEX degrading bacterium, screening method therefor and application of BTEX degrading bacterium
  • BTEX degrading bacterium, screening method therefor and application of BTEX degrading bacterium

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Effect test

Embodiment 1

[0052] Strain acquisition

[0053] The polluted soil was collected from a pesticide factory in Anhui and transported back to the laboratory in a brown bottle. The soil was added to the culture medium to prepare a mixture. After screening and domestication, a strain of highly efficient BTEX degrading bacteria was obtained, and the degradation of benzene series was verified for this strain. , it was found that the strain can efficiently degrade benzene, toluene, ethylbenzene and xylene, and the strain has stable passage characteristics.

[0054] Gram staining of the strain was negative, and the strain had no capsule and bacillus. Colony morphology on Luria-Bertani medium plates was regular, yellow, raised, opaque and smooth.

[0055] 16S rDNA sequencing was carried out on it, and the measured 16S rDNA sequences were compared by BLAST. The comparison results showed that the nucleotide sequence of the 16S rDNA of the strain ED-3 was similar to that of the nuclei of different stra...

Embodiment 2

[0070] Study on Degradation Characteristics of Bacterial Strains

[0071] Put 20mL of inorganic salt medium containing ethylbenzene in a 150mL serum bottle, after expanding ED-3 in Luria-Bertani medium, centrifuge at 8000r / min for 5min, and resuspend with 1% inorganic salt medium to wash away yeast powder Waiting for the carbon source, repeating twice, the total volume remains unchanged, and measuring the absorbance value at a wavelength of 600nm to ensure that the initial absorbance value in the degradation system is 0.1. In order to prevent the volatilization of ethylbenzene, cover and seal, shake culture at 28°C, 180rpm, and avoid light, change the concentration of ethylbenzene, pH value, yeast powder concentration, temperature, and take samples after 48 hours (the study on the influence of yeast powder takes samples at 36 hours) ), to determine the residual concentration of ethylbenzene.

[0072] Degradation characteristics results such as Figure 4 As shown, ED-3 can to...

Embodiment 3

[0074] Study on Degradation of Benzene Series in Water by Bacterial Strains

[0075] Put 20mL of inorganic salt medium containing 50mg / L benzene series (benzene, toluene, ethylbenzene, o-xylene, m / p-xylene) into a 150mL serum bottle, and expand culture of ED-3 in Luria-Bertani medium Finally, centrifuge at 8000r / min for 5min, resuspend with 1% inorganic salt medium, wash away carbon sources such as yeast powder, repeat twice and then measure the absorbance value under the condition of 600nm wavelength to ensure the initial absorbance in the degradation system The value is 0.1. In order to prevent the volatilization of benzene series, cover and seal, shake and culture at 28°C, 180 rpm, and avoid light, take samples regularly, and measure the residual concentration of benzene series.

[0076] Figure 8 It shows that ED-3 can degrade all BTEX substances, and has a good removal effect on benzene, toluene, and ethylbenzene. Benzene is completely consumed within 92 hours, toluene ...

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Abstract

The invention relates to a BTEX degrading bacterium, a screening method therefor and application of the BTEX degrading bacterium. The degrading bacterium belongs to beneficial bacilli, is called after Diaphorobacter sp. ED-3, is preserved in China Center for Type Culture Collection and has a preservation number of CCTCC NO. M 2021059. The screening method comprises the steps of taking and placing a contaminated soil sample in a closed serum bottle, and carrying out acclimatization, separation and purification, thereby obtaining the BTEX degrading bacterium. The bacterium ED-3 can completely degrade 100mg / L of ethylbenzene in 60h, metabolic accumulation of toxic intermediate products is absent, and no secondary pollutant is generated; and the bacterium can simultaneously degrade benzene, methylbenzene, o-xylene, m-xylene and p-xylene. The bacterium is a non-pathogenic bacterium and is stable in degradation effect; and the operating cost is relatively low, adverse impact on environments is low, and thus, the degrading bacterium has very good development and utilization prospects.

Description

technical field [0001] The invention belongs to the technical field of microbial treatment and degradation, and relates to a BTEX degrading bacterium and a screening method and application thereof. Background technique [0002] Benzene, toluene, ethylbenzene and xylene (Benzene, Toluene, Ethylbenzene, and Xylene, BTEX) are not only widely used as chemical raw materials in the synthesis of plastics and pesticides, but also important components of crude oil and petroleum products. Leaks in pipes and underground pipes often lead to contamination of soil and groundwater. BTEX has an obvious "three-to-one" effect, and many countries list it as a priority pollutant. In recent years, BTEX has been frequently detected in pesticide, petrochemical and other contaminated soil and groundwater, and its concentration is high and volatile. Especially in the process of excavation, transportation and remediation, secondary pollution will be generated. Such contaminated soil and groundwater ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/02C02F3/34B09C1/10C02F101/32C12R1/01
CPCC12N1/20C12N1/02C02F3/34B09C1/10C02F2101/322Y02W10/10
Inventor 崔长征张磊李莹莹单广波袁波张峰陈欣刘勇弟林匡飞罗启仕
Owner EAST CHINA UNIV OF SCI & TECH
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