Test strip for double-line detection of antibody after novel coronavirus vaccine inoculation

Abstract

The invention discloses a test strip for detecting novel coronavirus vaccination antibodies, which comprises a bottom plate, a sample pad, a combination pad, a reaction pad and a water absorption pad, the sample pad, the combination pad, the reaction pad and the water absorption pad are sequentially overlapped on the bottom plate, the reaction pad is provided with a control band and two detection bands, one of the detection bands is used for detecting whether an organism generates an immune reaction after the novel coronavirus vaccine is inoculated and generating a corresponding antibody; and the other detection band is used for detecting whether the neutralizing antibody generated by the body can reach a certain titer or not to form a protective effect. The test strip is good in sensitivity, good in detection performance, low in cost and simple in use method, and can be used for rapidly, simply and conveniently detecting the effect of the novel coronavirus vaccine after inoculation without professional technicians and professional equipment in multiple scenes such as hospitals, clinics and even at home; the sample demand is low; the effective vaccination rate of the vaccine can be improved, infection caused by poor vaccination effect is prevented, and the vaccine has a good clinical application prospect.

Description

technical field [0001] The invention relates to the technical field of rapid immunochromatographic detection, in particular to a test strip for double-line detection of antibodies after vaccination against the new coronavirus. Background technique [0002] The new type of coronavirus, namely severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the SARS coronavirus in 2003 and the MERS coronavirus in 2012 belong to the genus β of the Coronaviridae family. The virus is also listed by the World Health Organization as the seventh coronavirus to infect people. After the virus is infected, the incubation period is 7-14 days. After the onset, it is accompanied by pneumonia. The common symptoms are fever, cough and myalgia or fatigue; the less common symptoms are sputum, headache, hemoptysis, diarrhea, and dyspnea. The symptoms are similar to those of the common cold, and if not diagnosed and treated in time, there will be difficulty breathing and eventually death. SARS-C...

AI Technical Summary

Problems solved by technology

[0005] Chemiluminescence, colloidal gold / colloidal selenium, and enzyme-linked immunoassays can all be used for neutralizing antibody detection, but the inventors found for the first time that when using colloidal gold to detect neutralizing antibodies, the use of traditional control band coating strategies will lead to strip detection. The problem of invalid results; at the same time, a single test strip cannot explain the two levels of the body's response to the vaccine and the formation of protection at the same time, that is, whether the body produces neutralizing antibodies and whether the neutralizing antibodies can provide protection.

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