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Functionalized oligopeptide assembly as well as preparation method and application thereof

An assembly and functionalization technology, applied in chemical instruments and methods, preparation of organic compounds, preparation of aminohydroxy compounds, etc., can solve the problem that the morphology of the new assembly cannot obtain new functions, the uniformity of the new assembly is not good, and the limitations Assembly application and other issues, to achieve the effect of uniform size, good catalytic performance, simple and easy operation

Pending Publication Date: 2021-06-11
中科院过程工程研究所南京绿色制造产业创新研究院 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The above scheme adjusts the factors that affect the assembly behavior of the assembly, and the short peptide assembly behavior changes, which leads to the change of the shape of the assembly. A series of new assemblies are designed and prepared by changing the assembly environment, but the new assemblies are often not homogeneous. Good or cannot be prepared in large quantities, and new assemblies often only obtain changes in morphology and cannot obtain new functions, which limits the further application of assemblies

Method used

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  • Functionalized oligopeptide assembly as well as preparation method and application thereof
  • Functionalized oligopeptide assembly as well as preparation method and application thereof
  • Functionalized oligopeptide assembly as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0085] This example provides a functionalized short peptide assembly (Au@Fmoc-YK), which is prepared by the following preparation method:

[0086] (1) Weigh 0.27mg of Fmoc-YK and pour it into a 10mL plastic centrifuge tube, add 900μL×5 deionized water, shake for 5min to promote the dissolution of the sample, if there is any undissolved part, ultrasonicate for 3min to completely dissolve it, to avoid the existence of insoluble matter ;Use a pipette gun to pipette 2.43mL deionized water to dissolve the newly purchased unopened 1g package of HAuCl 4 , to prepare 1M mother solution, take 10μL mother solution and dilute to 1mL to get 10mM HAuCl 4 solution;

[0087] (2) Take 900μL Fmoc-YK solution in a 1.5mL plastic centrifuge tube, then add 100μL 10mM HAuCl 4 solution, shake for 3 minutes to mix the two solutions evenly; place the reaction at 25°C for 24 hours to obtain Au@Fmoc-YK nanospheres.

[0088] in, figure 1 For the SEM figure of the Fmoc-YK that embodiment 1 provides, ...

Embodiment 2

[0090] This example provides a functionalized short peptide assembly (Au@Fmoc-YK), which is prepared by the following preparation method:

[0091](1) Weigh 0.27mg of Fmoc-YK and pour it into a 1.5mL plastic centrifuge tube, add 900μL of deionized water, shake for 5 minutes to promote the dissolution of the sample, and if there is any undissolved part, ultrasonicate for 3 minutes to completely dissolve it to avoid the existence of insoluble matter; Use a pipette gun to pipette a total of 2.43mL of deionized water to dissolve the newly purchased unopened 1g package of HAuCl 4 , to prepare 1M mother solution, take 10μL mother solution and dilute to 1mL to get 10mM HAuCl 4 solution;

[0092] (2) Add 100μL 10mM HAuCl to 900μL Fmoc-YK solution 4 solution, shake for 3 minutes to mix the two solutions evenly; place the reaction at 25°C for 24 hours to obtain Au@Fmoc-YK nanospheres.

Embodiment 3

[0094] This example provides a functionalized short peptide assembly (Au@Fmoc-YK), which is prepared by the following preparation method:

[0095] (1) Weigh 0.53 mg of Fmoc-YK and pour it into a 1.5 mL plastic centrifuge tube, add 900 μL of deionized water, shake for 5 minutes to promote the dissolution of the sample, and if there is any undissolved part, ultrasonicate for 3 minutes to completely dissolve it to avoid the existence of insoluble matter; Use a pipette gun to pipette a total of 2.43mL of deionized water to dissolve the newly purchased unopened 1g package of HAuCl 4 , to prepare 1M mother solution, take 10μL mother solution and dilute to 1mL to get 10mM HAuCl 4 solution;

[0096] (2) Add 100μL 10mM HAuCl to 900μL Fmoc-YK solution 4 solution, shake for 3 minutes to mix the two solutions evenly; place the reaction at 25°C for 24 hours to obtain Au@Fmoc-YK nanospheres.

[0097] in, figure 2 For the SEM image of Au@Fmoc-YK (0.1mM) provided in Example 1, the size i...

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Abstract

The invention provides a functionalized oligopeptide assembly as well as a preparation method and application thereof. The functionalized oligopeptide assembly is prepared from the following raw materials: Fmoc dipeptide, chloroauric acid and a solvent, the Fmoc dipeptide is Fmoc-YK or Fmoc-YR, and the Fmoc dipeptide is Fmoc-YK. The functionalized oligopeptide assembly is prepared by regulating and controlling the Fmoc dipeptide assembly behavior through gold ions, 9-fluorenylmethoxycarbonyl (Fmoc) modified dipeptide and chloroauric acid are used as raw materials, no organic solvent or other components are introduced in the reaction process, and the dipeptide nanospheres prepared by the method are uniform in size and good in dispersity, and the prepared nano-particles have wide application prospects in the aspects of nano-material synthesis, drug delivery and catalysis.

Description

technical field [0001] The present invention relates to technical fields such as material science, synthesis of functionalized nanomaterials, shape regulation and catalysis of assemblies, in particular to a functionalized short peptide assembly and its preparation method and application, especially to a gold ion-regulated Fmoc dipeptide Assembly Behavior A method for preparing spherical functionalized short peptide assemblies. Background technique [0002] Molecular self-assembly refers to the process in which molecules spontaneously form ordered structures through non-covalent forces (electrostatic forces, hydrogen bonds, π-π stacking, and hydrophobic interactions) under certain thermodynamic and dynamic conditions. Inspired by functional molecular assemblies (proteins and DNA) in the human body, a large number of polypeptide or short peptide molecules with different sequences were designed and synthesized, and then used to assemble to form polypeptide or short peptide orde...

Claims

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Application Information

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IPC IPC(8): C07K5/065B82Y5/00B82Y40/00B01J31/04B01J35/08B01J35/02C07C213/02
CPCC07K5/06078B82Y5/00B82Y40/00B01J31/04C07C213/02B01J35/40B01J35/51C07C215/76Y02P20/55
Inventor 白硕王胜涛王安河
Owner 中科院过程工程研究所南京绿色制造产业创新研究院