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Application of lactobacillus plantarum gene in folic acid biological production

A Lactobacillus plantarum, gene technology, applied in the direction of plant gene improvement, microorganism-based methods, applications, etc., can solve problems such as differences in synthesis methods, and achieve the effect of improving folic acid production

Pending Publication Date: 2021-06-15
KUNMING UNIV OF SCI & TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Plants and some microorganisms can completely biosynthesize folic acid, but there are differences in the way they synthesize

Method used

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  • Application of lactobacillus plantarum gene in folic acid biological production
  • Application of lactobacillus plantarum gene in folic acid biological production
  • Application of lactobacillus plantarum gene in folic acid biological production

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Embodiment Construction

[0037] The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative work shall fall within the protection scope of the present invention.

[0038] 1. Using the method of homologous recombination, the folQ gene in the DHPPP pathway of folic acid synthesis of the L. plantarum YML4-3 strain was knocked out in a targeted manner, and the folQ gene knockout strain △folQ was obtained. The specific method is as follows:

[0039] (1), respectively prepare MRS broth (agar) medium, LB broth (agar) medium and improved CDM medium.

[0040] (2), carry out the experiment (Southern Bloting...

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Abstract

The invention discloses an application of a lactobacillus plantarum gene in folic acid biological production. By knocking out and replenishing a folQ gene in a folic acid synthesis DHPPP pathway of a YML4-3 strain and measuring a growth curve, pH, OD600, pathogenic bacteria inhibition capability and the like of each strain, it is determined that the folQ gene plays an important role in folic acid synthesis. The research gene is derived from food grade microorganisms, which is safe, folic acid is an indispensable nutrient element in the development process of microorganisms or human bodies, and proper supplementation is beneficial to healthy development, so that studies on the function and status of key enzyme genes for folic acid synthesis in the microorganisms are helpful to understand the mechanism of folic acid production, improve the yield of the folic acid, and lay a foundation for later development of folic acid fermented food.

Description

technical field [0001] The invention belongs to the technical field of folic acid synthesis, and in particular relates to the application of a gene of Lactobacillus plantarum in the biological production of folic acid. Background technique [0002] Folic acid, vitamin B9, is a water-soluble B vitamin. It is a combination of pterin, p-aminobenzoic acid (pABA) and one or more glutamic acids. It is a colorless and odorless yellow or orange crystal or crystalline powder, insoluble in ethanol, Diethyl ether and other organic solvents are soluble in alkaline solutions such as ammonia and potassium hydroxide. They are extremely unstable under acidic conditions and are easily destroyed under light conditions, especially ultraviolet radiation. Organisms generally require folic acid. However, different organisms The way the body obtains this nutrient varies. Animals cannot synthesize folic acid by themselves, but can obtain it through ingestion of food. Plants and some microorganis...

Claims

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Application Information

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IPC IPC(8): C12N15/74C12P17/18C12N15/55C12R1/25
CPCC12N15/746C12P17/182C12N9/14C12Y306/01Y02A50/30
Inventor 柳陈坚赵志敏李晓然
Owner KUNMING UNIV OF SCI & TECH