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Protein composition, application thereof and kit for early screening and diagnosis of ST segment elevation type myocardial infarction

A technology of myocardial infarction and composition, applied in the field of molecular biology, can solve the problems of early diagnosis and low prognostic value, clinical sensitivity and specificity limitations, etc.

Pending Publication Date: 2021-06-15
TEDA INT CARDIOVASCULAR HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these traditional or new biomarkers cannot fully reflect the progression and prognosis of ST-segment elevation myocardial infarction, and there are limitations in clinical sensitivity and specificity
For example, cTn T is highly specific in the heart, but it can take hours to increase its concentration above the 99th percentile reference upper limit (URL) of the assay, and it only increases during myocardial infarction, so it is important for The early diagnosis and prognostic value of the disease is very small

Method used

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  • Protein composition, application thereof and kit for early screening and diagnosis of ST segment elevation type myocardial infarction
  • Protein composition, application thereof and kit for early screening and diagnosis of ST segment elevation type myocardial infarction
  • Protein composition, application thereof and kit for early screening and diagnosis of ST segment elevation type myocardial infarction

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0097] 1. Collection of Human Plasma Samples

[0098] ST-segment elevation myocardial infarction group and unstable angina pectoris group were patients with ST-segment elevation myocardial infarction and unstable angina pectoris in emergency department, and patients with negative coronary angiography were the control group. 2ml of whole blood was collected from each patient's peripheral vein, and immediately centrifuged at 2500rpm for 15 minutes to separate the upper layer of plasma. The separated plasma was then divided into several equal parts, placed in plasma collection tubes, and stored in a -80°C refrigerator for testing.

[0099] 2. Proteomic detection

[0100] 1) Plasma proteome detection

[0101] Data independent acquisition (DIA) technology was used to detect proteins in each mixed sample of the three groups.

[0102] 2) Protein extraction

[0103] a) Take 40 μl of plasma from each sample and dilute ten-fold with binding buffer (kit: Binding Buffer).

[0104] b)...

Embodiment 2

[0182] Enzyme-linked immunosorbent assay (ELISA)

[0183]Prostaglandin H2 D-isomerase, H-cadherin / H-cadherin, and Rho GDP dissociation inhibitor 2 were verified in freshly collected plasma samples by ELISA. Experimental principle: The antibody of the protein to be tested is pre-embedded at the bottom of the 96-well plate. After the standard and sample are added, the protein to be tested will bind to the antibody. After removal of unbound substrate, a biotin-conjugated antibody to the protein of interest is added. After washing, anti-biotin conjugated horseradish peroxidase-labeled antibody (HRP) was added, and unbound HRP was removed by washing. After adding a chromogenic reagent to terminate the reaction, measure the absorbance of the liquid.

[0184] Validated Prostaglandin H2 D-isomerase, Include:

[0185] Multi-well plate coated with prostaglandin H2 D-isomerase antibody

[0186] Pure prostaglandin H2 D-isomerase as a standard

[0187] Biotin-labeled antibody

[...

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Abstract

The invention provides a protein composition, application thereof and a kit for early screening and diagnosis of ST segment elevation type myocardial infarction. The composition is composed of prostaglandin H2D-isomerase, H-cadherin / H-cadherin and Rho GDP dissociation inhibition factor 2. The detection and functions of the prostaglandin H2D-isomerase, H-cadherin / H-cadherin and Rho GDP dissociation inhibition factor 2 are preliminarily studied by utilizing a modern biological technology and bioinformatics analysis; the high-concentration prostaglandin H2D-isomerase, H-cadherin / H-cadherin and the Rho GDP dissociation inhibition factor 2 can be used for early prediction of ST segment elevation type myocardial infarction, and can be applied to preparation of detection preparations or medical instruments for early intervention. The protein composition and the kit have relatively good clinical diagnosis efficiency and potential huge application value on the early ST segment elevation type myocardial infarction.

Description

technical field [0001] The invention relates to the field of molecular biology, in particular to a protein composition and its application and an ST-segment elevated myocardial infarction early screening diagnostic kit. Background technique [0002] Acute coronary syndrome refers to the acute ischemic syndrome of the heart caused by the rupture or erosion of unstable atherosclerotic plaques in coronary arteries secondary to the formation of fresh thrombus. It is divided into ST-segment elevation myocardial infarction, non-ST-segment elevation High-grade myocardial infarction and unstable angina. Coronary artery disease causing acute coronary syndrome remains one of the leading causes of death worldwide. Among them, ST-segment elevation myocardial infarction refers to acute myocardial ischemic necrosis, which is caused by a sharp decrease or interruption of coronary blood flow, resulting in severe and persistent acute ischemia of the corresponding myocardium. The incidence ...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/58G01N33/573
CPCG01N33/6893G01N33/573G01N33/58G01N33/581G01N2800/324G01N2333/99G01N2333/4727G01N2333/4704
Inventor 何国伟薛红梅宋昱张云强侯海涛杨沁王君
Owner TEDA INT CARDIOVASCULAR HOSPITAL
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