Indocyanine green hydrogel fluorescent calibration plate as well as preparation method and application thereof

Abstract

The invention provides an indocyanine green hydrogel fluorescent calibration plate as well as a preparation method and application thereof. The indocyanine green hydrogel fluorescent calibration plate is prepared with the method comprising following steps: (1) preparing a mixed solution of polyvinyl alcohol, water and dimethyl sulfoxide; and (2) dissolving indocyanine green in the mixed solution prepared in the step (1) to generate a stable three-dimensional network structure, and preparing the long-acting and stable indocyanine green hydrogel fluorescent calibration plate with a certain shape by regulating and controlling the temperature. The indocyanine green hydrogel fluorescent calibration plate has the advantages of high fluorescence intensity stability, reusability, high light transmittance, convenience in use and the like, and has great application potential in near-infrared fluorescence imaging.

Description

technical field [0001] The present invention relates to the field of near-infrared surgical navigation imaging, and in particular to an indocyanine green hydrogel fluorescence calibration plate and its preparation method and application. Background technique [0002] Indocyanine green (Indocyanine green, ICG) is a hydrophilic fluorescent tracer with a molecular weight of about 776Da. Under the irradiation of near-infrared fluorescence with a wavelength of 780nm, it can emit fluorescence with a wavelength of 830nm, which has good penetration, and ICG can be used for fluorescence detection in some deep tissues. When ICG enters the blood, it can quickly combine with plasma proteins to form protein-coated ICG nanomolecules, which can be enriched in tumor tissues through the EPR effect and phagocytized by the reticuloendothelial phagocytic system, thereby participating in tumor visualization and lymph node tracking. However, before using fluorescence navigation equipment for tum...

AI Technical Summary

Problems solved by technology

[0003] ICG, as the only near-infrared fluorescent tracer currently approved for clinical practice, has a fluorescence quantum yield of 0.5% in aqueous solution. Indocyanine green solution is easily oxidized and photobleached under the conditions of light and oxygen, resulting in fluorescent signals. disappear

Due to the problems of photobleaching and degradation of ICG solution, the ICG concentration gradient solution cannot be stored stably and reused. Therefore, it is necessary to prepare ICG gradient concentration solution before each navigation operation, which inevitably leads to the waste of ICG and the complexity of surgical preparation.

[0004] It can be seen that the development of long-acting and stable indocyanine green system can make up for the deficiency of indocyanine green in actual use, and has high scientific research and clinical application value, but there is currently no effective solution in the industry

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