Light-digital PCR chamber and light-digital PCR device using same

Abstract

The present invention relates to a light-digital PCR chamber and a light-digital PCR device. The light-digital PCR chamber comprises: a transparent substrate; a metal thin film layer formed on the transparent substrate; a light shielding layer formed on the metal thin film layer; and a microchannel structure formed on the light shielding layer. The light-digital PCR device comprises a laminate comprising a transparent substrate, a metal thin film layer formed on the transparent substrate, and a light-shielding layer formed on the metal thin film layer.

Description

technical field [0001] The present invention relates to an optical digital PCR chamber and an optical digital PCR device using the chamber. Background technique [0002] The polymerase chain reaction (PCR) method is a technique for mass amplification of specific regions of nucleic acid (DNA or RNA) in vitro. PCR equipment using this technology is used in hospitals and research institutions to amplify small amounts of DNA in blood, and the PCR equipment is also used to diagnose various diseases such as malaria, tuberculosis, and hepatitis. [0003] In recent years, the PCR method has been developed into real-time PCR, digital PCR, etc., which provide results with various conveniences that cannot be provided by existing PCR, such as real-time diagnosis, high sensitivity, digitization of measurement results, and high accuracy. [0004] Among them, digital PCR is a new method for detection and quantitative analysis of nucleic acid (DNA or RNA), and the digital PCR reaction is p...

AI Technical Summary

Problems solved by technology

However, since the existing PCR device uses electric energy as a thermal energy source, it takes 1 hour or more to complete the nucleic acid amplification reaction, and requires components such as a heating device, a radiator, etc.

As a result, there has been a problem in that the equipment takes up a large volume and is inconvenient, and therefore, it is somewhat inconvenient to apply the existing PCR device to fields requiring rapid diagnosis of diseases

However, applying an electric current to generate heat on a gold film requires a longer detection time compared to light energy-based PCR, which limits

Also, in Korean Patent Laid-Open No. 2017-0106995, light energy-based PCR is disclosed, but since the PCR reaction is performed in a liquid phase, there are problems in that multiple diagnosis is not possible in one chamber, and it is impossible to recover Primers used

[0009] In particular, existing PCR devices have limitations in terms of high price, large-scale equipment, long detection time (more than 1 hour), inconvenience to carry, and on-site instant diagnosis. Therefore, to address these issues, PCR studies have been steadily performed

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