Application of arbuscular mycorrhizal fungi in salt stress resistance of carica papaya

A technology of arbuscular mycorrhizal fungi and papaya, applied in application, seed coating/seed dressing, cultivation and other directions, can solve the problems of grana disorder, destruction of electron transfer, swelling of chloroplast thylakoid, etc., to promote growth , enhance salt resistance, improve the effect of soil environment

Active Publication Date: 2021-06-25
HUIZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] (2) Salt stress can also inhibit the photosynthesis of plants. The water loss caused by unbalanced salt stress in plants reduces the stomatal conductance to reduce transpiration and water loss, resulting in a decrease in the supply of CO2 required for the dark reaction of photosynthesis; plant chloroplasts Sensitive to salt stress. Under salt stress, chloroplast thylakoids will swell, decrease in number, and grana disorder, etc., which will affect the activity of chlorophyll synthase, resulting in a decrease in chlorophyll content; salt stress will also destroy photosystem II reaction center QA to QB electron transfer
[0009] After searching the literature, no published literature has been found on the mechanism of papaya and arbuscular mycorrhizal symbiosis and mycorrhizal seedling resistance to salt stress

Method used

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  • Application of arbuscular mycorrhizal fungi in salt stress resistance of carica papaya
  • Application of arbuscular mycorrhizal fungi in salt stress resistance of carica papaya
  • Application of arbuscular mycorrhizal fungi in salt stress resistance of carica papaya

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Embodiment

[0055] 1. Test materials

[0056]The transgenic papaya Huanong No. 1 and conventional papaya Suizhonghong were obtained from mature fruits. After peeling off the outer coat, the seeds were surface-sterilized with 0.1% HgCl solution for 5 minutes, rinsed with distilled water for 3 times, soaked in sterile water for 24 hours, and placed Germinate on moist sterile filter paper in a light incubator (light time 16h, temperature 25°C, humidity >90%). After the seeds germinated, they were sown in plastic pots with 1000 g of culture soil, and 5 seeds were sown in each pot. After 2 weeks, the seedlings were thinned out, and one seedling was kept in each pot;

[0057] Rhizophagus intraradices was isolated from the soil of papaya planting base in Huidong as the tested fungus. After propagating with clover, obtain clover rhizosphere soil containing spores, hyphae and infected root segments as bacterial agent (50 spores / g soil). When sowing, the inoculation process applies 10g fungicide...

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Abstract

The invention discloses application of arbuscular mycorrhizal fungi in salt stress resistance of carica papaya. The invention relates to the technical field of plant protection. The arbuscular mycorrhizal fungi are rhizophagus intraradices. The method is realized by any one of the following modes: (1) mixing rhizophagus intraradices with clover and sterilized soil, and then carrying out propagation to obtain a root soil mixture containing spores, hyphae and infected root segments, so as to obtain a target microbial agent; mixing a target microbial agent with the soil where the carica papaya seeds are to be sown, and sowing the carica papaya seeds or seedlings; (2) mixing rhizophagus intraradices with a matrix to obtain a mixed matrix, and sowing the carica papaya seeds or seedlings on the matrix. By adopting the technical scheme, the plant growth and stress resistance of the carica papaya under the salt stress condition are promoted.

Description

technical field [0001] The invention relates to the technical field of plant protection, in particular to the application of an arbuscular mycorrhizal fungus in resisting salt stress of papaya. Background technique [0002] In the north of the Yangtze River and in many coastal areas in China, the saline-alkali content in the soil is often too high, causing harm to plants, as follows: [0003] (1) Excessive salinity in the soil will harm the growth and development of plants and cause physiological drought in plants. The accumulation of salt reduces the water potential of the soil, making it difficult for plant roots to absorb water and cannot absorb enough water. If there is an imbalance between water uptake and transpiration by plants, the plants will wilt and even die. [0004] (2) Salt stress can also inhibit the photosynthesis of plants. The water loss caused by unbalanced salt stress in plants reduces the stomatal conductance to reduce transpiration and water loss, res...

Claims

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Application Information

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IPC IPC(8): A01C1/06A01G17/00
CPCA01C1/06A01G17/005
Inventor 陈勇智谢海伟曾令达李佳赛王晓旭黄冰冰郭乐榆龙彩玲
Owner HUIZHOU UNIV
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