Application of arbuscular mycorrhizal fungi in salt stress resistance of carica papaya
A technology of arbuscular mycorrhizal fungi and papaya, applied in application, seed coating/seed dressing, cultivation and other directions, can solve the problems of grana disorder, destruction of electron transfer, swelling of chloroplast thylakoid, etc., to promote growth , enhance salt resistance, improve the effect of soil environment
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[0055] 1. Test materials
[0056]The transgenic papaya Huanong No. 1 and conventional papaya Suizhonghong were obtained from mature fruits. After peeling off the outer coat, the seeds were surface-sterilized with 0.1% HgCl solution for 5 minutes, rinsed with distilled water for 3 times, soaked in sterile water for 24 hours, and placed Germinate on moist sterile filter paper in a light incubator (light time 16h, temperature 25°C, humidity >90%). After the seeds germinated, they were sown in plastic pots with 1000 g of culture soil, and 5 seeds were sown in each pot. After 2 weeks, the seedlings were thinned out, and one seedling was kept in each pot;
[0057] Rhizophagus intraradices was isolated from the soil of papaya planting base in Huidong as the tested fungus. After propagating with clover, obtain clover rhizosphere soil containing spores, hyphae and infected root segments as bacterial agent (50 spores / g soil). When sowing, the inoculation process applies 10g fungicide...
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