Culture medium for culture, preservation and rejuvenation of edible fungus strains, preparation method and application of culture medium
A technology for culturing bacteria and culture medium, applied in the field of culture medium, it can solve the problems of affecting quality, prone to degradation or aging, production and processing effects, etc.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0028] Weigh 20g of agar, 200g of potatoes, 20g of glucose, KH 2 PO 4 1.5g, MgSO 4 0.5g, V b A small amount (about 0.01g), peel and cut potatoes (cut to a length and width close to 1cm), add 1000mL deionized water and boil for 15-20min, filter the potato extract with gauze, then add the remaining substances, heat and stir evenly To 1000mL, divide into two 1000mL Erlenmeyer flasks, each 500mL; weigh 5g of 200-300 mesh activated carbon, divide into two 50ml Erlenmeyer flasks, each 2.5g, seal with a sealing film and place under high pressure In the bacteria pot, sterilize at 121°C for 18 minutes. After the sterilization is completed, take out the triangular flask and place it on the ultra-clean workbench. Shake well, then divide into sterile petri dishes, and obtain the culture medium after cooling and solidifying.
[0029] The culture medium can be used in the processes of cultivation, preservation and rejuvenation of edible fungus strains.
[0030] Using Ganoderma lucidu...
Embodiment 2
[0037] Weigh 15g of yeast powder, 20g of glucose, KH 2 PO 4 1.5g, MgSO 4 0.5g, V b A small amount (about 0.01g), add 1000mL deionized water and boil for 15-20min, then weigh 20g of agar powder and continue to add and stir to dissolve, set the volume to 1000mL, transfer to a 1000mL Erlenmeyer flask, then weigh 5g of 200-300 mesh Add activated carbon into the conical flask, transfer the conical flask to a magnetic stirrer, add the rotor to the conical flask, stir the medium at a stirring speed of 500rpm, and use the intelligent liquid dispensing pump to dispense the medium to 18*180mm In a glass test tube, cover it with a rubber stopper and sterilize it at 121°C for 18 minutes. After the sterilization is completed, take out the test tube and shake it gently to mix well, then place the test tube flat on a slope with an appropriate inclination. base. In addition to the preservation of the edible fungus strains, the culture medium can also be used in the cultivation and rejuv...
Embodiment 3
[0039] Weigh 15g of yeast powder, 20g of glucose, KH 2 PO 4 1.5g, MgSO 4 0.5g, V b A small amount (about 0.01g), add 500mL deionized water and boil for 15-20min, transfer to a 1L Erlenmeyer flask, then weigh 5g of 200-300 mesh activated carbon, add it to the Erlenmeyer flask, stir and shake with a glass rod Evenly, weigh 20g of agar powder into another 1L Erlenmeyer flask, add 500mL of deionized water and transfer to a water bath for heating and dissolving. After the medium and agar are sealed, sterilize at 121°C for 20min, and transfer to an ultra-clean Mix and shake well behind the counter, pour it into a flat plate, and wait for it to solidify.
[0040] The degraded Ganoderma lucidum was transferred to the plate, and after a few days, mycelium could germinate and grow, while the control plate medium (without adding activated carbon) mycelia did not germinate, such as figure 2 As shown, it is shown that the culture medium of this embodiment can restore the vitality of...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More