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Pharmaceutical composition for alleviating eye fatigue, containing, as active ingredients, luteolin-7-o-diglucuronide and apigenin-7-o-diglucuronide isolated from perilla frutescens (l.) britton var. acuta (thunb.) kudo leaf extract

A technology of glucuronide and perilla leaf extract, which is applied in the direction of medical preparations containing active ingredients, drug combinations, organic active ingredients, etc., can solve the problems of decreased dark adaptation ability and easy fatigue of the eyes, and achieve improved eye health. The effect of fatigue

Inactive Publication Date: 2021-06-25
JEONNAM BIOIND FOUND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In a highly developed mechanical civilization, the eyes are prone to fatigue due to various environmental pollution, excessive viewing of TV, excessive use of personal computers and electronic entertainment equipment, etc., and when driving or working at night, vision loss such as decreased dark adaptation ability will occur The phenomenon

Method used

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  • Pharmaceutical composition for alleviating eye fatigue, containing, as active ingredients, luteolin-7-o-diglucuronide and apigenin-7-o-diglucuronide isolated from perilla frutescens (l.) britton var. acuta (thunb.) kudo leaf extract
  • Pharmaceutical composition for alleviating eye fatigue, containing, as active ingredients, luteolin-7-o-diglucuronide and apigenin-7-o-diglucuronide isolated from perilla frutescens (l.) britton var. acuta (thunb.) kudo leaf extract
  • Pharmaceutical composition for alleviating eye fatigue, containing, as active ingredients, luteolin-7-o-diglucuronide and apigenin-7-o-diglucuronide isolated from perilla frutescens (l.) britton var. acuta (thunb.) kudo leaf extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1. The effect of perilla extract in aortic smooth muscle cells (in vitro) and rabbit ciliary muscle (in vitro), adjusting proximal point (human body applicable test)

[0036] 1. Preparation of hot water extraction of perilla leaves

[0037] 3 kg of perilla leaves (dried leaves) were extracted with hot water at 100° C. for 3 hours using 10 times as much distilled water. The water extract extracted above was concentrated under reduced pressure and freeze-dried to obtain 650 g of hot water freeze-dried perilla leaves.

[0038] 2. Use HPLC to analyze the components of perilla extract

[0039] The HPLC device used in the component analysis of perilla leaf hot water extract is YL 9100HPLC system, and the chromatographic column uses Triart C18 plus (250x 4.6mm, 5um, YNC Co., Ltd.). Mobile phase is methanol (mobile phase A) and distilled water for HPLC (mobile phase B, 0.1% formic acid), and the ratio of methanol is adjusted from 30% (0-10 minutes) to 30% ~ 50% (10...

Embodiment 2

[0060] Example 2. Effects of luteolin-7-O-diglucuronide and apigenin-7-O-diglucuronide contained in perilla leaf extract on ciliary muscle isolated from SD rats Cellular Eyestrain Effects

[0061] 1. Preparation of perilla leaf hot water extract and isolation of flavonoid glycoside compounds

[0062] 1-1. Preparation of perilla leaf water extract

[0063] The dried perilla leaves were subjected to hot water extraction at 100° C. for 3 hours with distilled water. The above extract was concentrated under reduced pressure and then freeze-dried and spray-dried to obtain hot water freeze-dried perilla leaves (650 g, 21.6%) and spray-dried (669 g, 22.4%).

[0064] 1-2. Separation of active ingredients using formaldehyde-based resin HP-20 (Diaion HP-20resin)

[0065] In order to separate the effective components from perilla leaf extract, 2L of perilla leaf hot water extract was added to formaldehyde-based resin HP-20, and water and methanol were sequentially mixed at 30:70, 50:50...

Embodiment 3

[0100] Embodiment 3: the human body application test of eye regulation

[0101] 1. Applicable test method for human body

[0102] This study was conducted under the approval of the Medical Device Clinical Trials Review Board of D University Hospital (IRB license number: DSGOH-033).

[0103] A total of 35 adult men and women aged 18+ to 60+ were screened, and the subjects voluntarily gave their written consent after receiving an explanatory note for the study. No congenital or chronic diseases; results of medical diagnosis and treatment, no pathological symptoms or characteristics; results of blood tests and vital signs within the normal range; A total of 30 people were finally studied. The study was conducted by taking perilla extract and placebo control group, and performing close proximity task (VDT) for 2 hours before the final dose.

[0104] 2. Adjust near point (Near Point of Accomodation) check

[0105] Adjusting the near point is measured as follows: In the state of...

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Abstract

Provided is a functional health food product exhibiting eye fatigue prevention and alleviation by means of luteolin-7-O-diglucuronide and apigenin-7-O-diglucuronide compounds contained in a Perilla frutescens (L.) Britton var. acuta (Thunb.) Kudo extract. It has been confirmed that the compounds of luteolin-7-O-diglucuronide and apigenin-7-O-diglucuronide are contained in equal amounts as active ingredients of freeze-dried and spray-dried products of the leaves of Perilla frutescens (L.) Britton var. acuta (Thunb.) Kudo of the present invention. In ciliary muscle cells isolated from rat eyes (rCSMCs), the amounts of NO and cGMP, which are associated with eye relaxation, are increased and the amount of [Ca2+]i ion is decreased. In addition, the amount of cGMP increased in animal experiments. These compounds increase the amount of cGMP in ciliary muscle cells isolated from rat eyes, thereby being usable in a functional health food composition useful for the prevention and alleviation of eye fatigue.

Description

technical field [0001] The present invention relates to a method comprising luteolin-7-O-diglucuronide (luteolin-7-O-diglucuronide) and apigenin-7-O-diglucuronide extracted from Perilla leaf extract (apigenin-7-O-diglucuronide) is an active ingredient of a pharmaceutical composition for improving eye fatigue and a health functional food composition. Background technique [0002] Basil (scientific name: Perilla frutescens (L.) Brittonvar.acuta (Thunb.) Kudo), as an annual medicinal plant, belongs to Angiosperm, Dicotyledoneae, Labiatae in biological classification, and is distributed in Korea and China. [0003] The stem is upright and square, the whole plant is purple and has a fragrance, the leaves grow in pairs, the leaves are broadly ovate, the apex is short and pointed, the base is round or roughly wedge-shaped and the edges are serrated, both sides are pilose, especially the longest and short with long Plumber, petiole is long. Flowers bloom in lavender from August t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/535A61K31/7048A61K9/20A61K9/48A61P27/02
CPCA61K9/20A61K31/7048A61P27/02A61K9/48A61K36/535A61K2236/331A61K2236/51
Inventor 崔彻雄金龙姜厚远A·曹吴乧理金瑜真林沼廷李涩琪李圭玉朴成伦柳根昌成乐宣
Owner JEONNAM BIOIND FOUND
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