Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of entoderm stem cell in prevention and/ or treatment of liver immune disorder disease

A technology of immune disorders and stem cells, applied in the field of biomedicine, can solve problems such as complex sources of MSCs

Pending Publication Date: 2021-06-29
CENT FOR EXCELLENCE IN MOLECULAR CELL SCI CHINESE ACAD OF SCI +1
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, there are also problems such as complex sources of MSCs and large differences in MSCs from different sources.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of entoderm stem cell in prevention and/ or treatment of liver immune disorder disease
  • Application of entoderm stem cell in prevention and/ or treatment of liver immune disorder disease
  • Application of entoderm stem cell in prevention and/ or treatment of liver immune disorder disease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0114] Example 1 Efficacy evaluation of hEnSCs transplantation in the treatment of acute liver failure in a rat model

[0115] In order to comprehensively evaluate the efficacy of hEnSCs in the treatment of acute liver failure, different animal models, including mice and pigs, were used to measure the efficacy of hEnSCs in the treatment of acute liver failure ( figure 1 A). In a commonly used rat model, D-GalN was used as a drug to induce acute liver failure, and hEnSCs cells were transplanted through the hepatic portal vein 24 hours after induction ( figure 1 B), then during the acute liver failure morbidity period, that is, within a week, some rats are collected blood, liver tissue and other sampling work, and record the survival curve of the rats that do not collect blood (such as figure 1 A). Rats treated with hEnSCs transplantation can be seen in the survival curve within one week, which has a significantly improved survival rate compared with untreated rats, and is sli...

Embodiment 2

[0116] Example 2 Evaluation of the curative effect of hEnSCs transplantation in the treatment of acute liver failure in a miniature pig model

[0117] In order to be closer to the human situation, in addition to experiments on rat models, the curative effect of hEnSCs was verified again on large animals. Therefore, a commonly used large animal model of acute liver failure was selected, which was consistent with rats, that is, the acute liver failure model of pigs induced by D-GalN. The pig strain is Chinese Bama Pigs. Due to the larger size of pigs, more cells need to be transplanted, and the number of hEnSCs transplanted by each pig is about 5X10 8 indivual. A total of 10 minipigs were transplanted, of which 8 survived ( figure 2 A, 2B). In terms of liver function indexes of pigs transplanted with hEnSCs, alanine aminotransferase (ALT) and aspartate aminotransferase (AST) did not decrease significantly on Day 1 and Day 2, but blood ammonia (NH 3 ) and prothrombin time (...

Embodiment 3

[0119] Example 3 Comparative evaluation of the curative effect of hEnSCs and other types of cells in different acute liver failure models

[0120] In order to further verify the therapeutic effect of hEnSCs on acute liver failure, hEnSCs were compared in detail with MSCs known to have therapeutic effects. At the level of liver function indicators, EnSCs performed slightly better than MSCs, but not statistically significant ( image 3A). Immunohistochemical section also reflected that these two kinds of cells can effectively protect liver structure and reduce inflammatory infiltration ( image 3 B). Not only that, in addition to the D-GalN-induced rat and pig models, the efficacy of hEnSCs has continued to be validated in other models of acute liver failure and compared with other cell types. Concanavalin A (ConA)-induced mouse (Mouse) acute liver failure model, and D-galactoside hydrochloride (D-Galactosamine hydrochloride, D-GalN) plus lipopolysaccharide (Lipopolysaccharid...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides application of an entoderm stem cell in prevention and / or treatment of a liver immune disorder disease. Specifically, the invention provides a composition or a preparation for preventing and / or treating the liver immune disorder disease. The entoderm stem cell disclosed by the invention can obviously improve the survival rate of an animal, in addition, the entoderm stem cell performs a protection function for the liver, meanwhile, the entoderm stem cell has a function of inhibiting apoptosis and inflammation, and all the above functions of the entoderm stem cell are favorable for survival of the animal, recovery of liver functions and regeneration of liver tissues.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to the application of endoderm stem cells in the prevention and / or treatment of liver immune disorders. Background technique [0002] Acute liver failure (ALF) is an acute syndrome with high mortality, which is characterized by rapid necroptosis and apoptosis of a large number of hepatic parenchymal cells in a short period of time, and the loss of original liver function. And accompanied by intrahepatic immune system disorders, hepatic encephalopathy and possible multiple organ failure and other complications. [0003] The causes of acute liver failure include the following three types: those caused by hepatitis viruses such as hepatitis A, B and E (Hepatitis A, B&E); those caused by improper medication or poisoning, such as Chinese herbal medicine (Chinese Herbal Medicine), acetaminophen (Acetaminophen) ); other factors such as ischemic hepatocellular injury (Ischemic Hepatocellular Inj...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K35/545A61K35/28A61K35/407A61P1/16
CPCA61K35/545A61K35/28A61K35/407A61P1/16A61K2300/00A61K35/12C12N5/06Y02A50/30
Inventor 程新施晓雷徐艺林王经琳任昊桢
Owner CENT FOR EXCELLENCE IN MOLECULAR CELL SCI CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com