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Method for degrading glyphosate pesticide by using lactobacillus alkaline phosphatase

A phosphatase and glyphosate technology, applied in the field of pesticide degradation, can solve problems such as pollution, endangering human health, increasing the risk of cancer, cardiovascular disease and infertility

Inactive Publication Date: 2021-07-02
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, the pollution caused by glyphosate pesticide residues has been closely watched by people. Many reports have confirmed that glyphosate pesticides have caused serious pollution to soil, water quality and other ecological environments and food, and also endangered human health through enrichment. Glyphosate can cause varying degrees of damage to multiple human systems, and can even increase the risk of cancer, cardiovascular disease, and infertility

Method used

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  • Method for degrading glyphosate pesticide by using lactobacillus alkaline phosphatase
  • Method for degrading glyphosate pesticide by using lactobacillus alkaline phosphatase
  • Method for degrading glyphosate pesticide by using lactobacillus alkaline phosphatase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] (1) culturing lactic acid bacteria and adopting ultrasonic pulverization method to obtain alkaline phosphatase;

[0021] (2) Detect the residue of glyphosate pesticide by high performance liquid chromatography;

[0022] (3) Discover the mode of action of glyphosate and ALP by molecular docking Auto Dock technology, and visualize the information such as the action site and binding force;

[0023] (4) ALP was used to degrade glyphosate pesticides at a concentration of 0.5 mg / L, respectively, and the degradation rate was analyzed in combination with the liquid phase peak area after 4 hours.

Embodiment 2

[0025] (1) culturing lactic acid bacteria and adopting ultrasonic pulverization method to obtain alkaline phosphatase;

[0026] (2) Detect the residue of glyphosate pesticide by high performance liquid chromatography;

[0027] (3) Discover the mode of action of glyphosate and ALP by molecular docking Auto Dock technology, and visualize the information such as the action site and binding force;

[0028] (4) Glyphosate pesticides with a concentration of 1 mg / L were degraded by ALP respectively, and the degradation rate was analyzed in combination with the peak area of ​​the liquid phase after 4 hours.

Embodiment 3

[0030] (1) culturing lactic acid bacteria and adopting ultrasonic pulverization method to obtain alkaline phosphatase;

[0031] (2) Detect the residue of glyphosate pesticide by high performance liquid chromatography;

[0032] (3) Discover the mode of action of glyphosate and ALP by molecular docking Auto Dock technology, and visualize the information such as the action site and binding force;

[0033] (4) Glyphosate pesticides with a concentration of 2 mg / L were degraded by ALP respectively, and the degradation rate was analyzed in combination with the liquid phase peak area after 4 hours.

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Abstract

The invention aims to extract alkaline phosphatase (ALP) from food-grade strain lactic acid bacteria, apply the ALP to degradation of pesticide glyphosate, and explore the mechanism of the alkaline phosphatase for promoting the degradation of the glyphosate pesticide and the interaction mode of the ALP and the glyphosate pesticide by using a computer-aided molecular simulation technology. The invention develops a method for degrading glyphosate pesticide by using lactobacillus alkaline phosphatase.

Description

technical field [0001] The invention relates to the technical field of pesticide degradation, and provides a method for degrading glyphosate pesticide by utilizing lactic acid bacteria alkaline phosphatase. Background technique [0002] As an important means of preventing and controlling crop diseases and insect pests, pesticides play an important role in the development of agriculture around the world. In recent years, the demand for pesticides has also been increasing. The usage amount is as high as 320,000 tons per year on average. Organophosphorus pesticides are widely used all over the world due to the advantages of wide variety, wide application and strong pharmacophore. [0003] Glyphosate has become one of the most widely used pesticides in the past three decades due to its advantages of being quickly absorbed by plants and reducing components. It has been widely used in many fields such as weed control, especially in genetically modified crops. . In recent years, ...

Claims

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Application Information

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IPC IPC(8): A62D3/02A62D101/04A62D101/26
CPCA62D3/02A62D2101/04A62D2101/26
Inventor 王玉堂任海斌张英华修红霞杨慧慧
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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