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Rose palmata, fruity mushroom and cultivation method thereof

A method for cultivating and a technique for rose ears, applied in the field of microorganisms, can solve the problems of inability to commercialize production and low yield, and achieve the effects of shortening the fruiting cycle, fast nutrient transformation, and simple operation.

Active Publication Date: 2021-07-09
河南果菇蓉生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in this thesis, the mushroom is only artificially cultivated experimentally in the laboratory, and the output is very low, so it cannot be commercially produced.

Method used

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  • Rose palmata, fruity mushroom and cultivation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The separation and identification of embodiment 1 palmate rose ear

[0034] In August 2020, the inventor Tuligul collected the strain of Rosa palmatum in Shansongling, Jiaohe City, Jilin Province. After tissue isolation and culture, biological characteristics observation, detection and identification, it was determined to be the fungus of Rosa palmatum Silk, preserved in the General Microorganism Center (CGMCC) of the China Committee for the Collection of Microbial Cultures (CGMCC), the definition symbol is GGR-001, the address is No. 1, Beichen West Road, Chaoyang District, Beijing, China, the preservation number is CGMCC No. 21941, and the preservation date for April 2, 2021.

Embodiment 2

[0035] The cultivation of embodiment 2 test tube parent species

[0036]Wash and peel 200g of potatoes and cut into thin slices, add 1000mL of water to boil for 20min, filter with gauze, take the filtrate and make up water to 1000mL, add 22g of agar powder, 0.5g of magnesium sulfate, 0.8g of potassium dihydrogen phosphate, 2.5 of peptone g, 25g of glucose and 0.5g of vitamin B1 powder, heat again until the agar powder is fully melted and then put into test tubes, the amount of each test tube is 1 / 5 of the length of the test tube, after filling, put a sterile cotton plug or silica gel stopper, use plastic Seal the paper well, tie a bundle for every 7-10 test tubes, and tie them up with strings; then put them into a high-pressure steam sterilizer, heat to 100°C, discharge cold air, and heat again to a pressure of 0.1-0.12MPa (pot (injection temperature reaches 121°C) for 30 minutes. When the pressure drops to 0, slowly release the gas in the pot. When the culture medium is not s...

Embodiment 3

[0037] Embodiment 3 solid bacterial classification expansion culture bacterial classification

[0038] Including the following steps:

[0039] (1) Original seed culture: put 400g of sorghum solid medium in a 500mL glass container, cover with a sterile air-permeable film, sterilize at 118-122°C and pressure 0.12-0.14MPa for 90-120min, and wait for the inside of the bottle to cool When reaching 22-24 ℃, insert the parent species of the test tube of embodiment 2 under aseptic conditions, and the inoculum size is 10cm 3 The mother species of the test tube obtained in Example 3 was cultivated for 40 days at 20-22°C to obtain the original species;

[0040] The preparation method of the sorghum solid medium is as follows: adding quicklime to water at a temperature of 100° C. to adjust the pH value to 8.5, putting the sorghum into the water, submerging the top surface of the sorghum by 10 cm, boiling for 20 minutes until there is no white heart in the center of the sorghum, and then ...

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Abstract

The invention belongs to the technical field of microorganisms, and particularly relates to rose palmata, fruity mushroom and a cultivation method thereof. The rose palmata is preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC No.21941. The fruity mushroom cultivation method comprises the following steps: (1) culture of a test tube mother strain is conducted, specifically, a test tube filled with a mother strain culture medium is sterilized, a palmarosa palmosa tissue block is inoculated under an aseptic condition, and culturing is conducted at 20-22 DEG C for 20-25 days to obtain the test tube mother strain; (2) enlarged culture of the strain is conducted, specifically, the test tube mother strain obtained in the step (1) is subjected to enlarged culture to obtain an enlarged culture strain; and (3) fruiting culture is conducted, specifically, the enlarged culture strain in the step (2) is subjected to bag cultivation or bottle cultivation, and fruiting is conducted to obtain a finished product. The fruity mushroom obtained through the method is high in nutrient content and short in cultivation time, the biological conversion rate reaches up to 70% or above, and the method can be widely applied to industrial production.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a palm-shaped fungus, a fruit-flavored mushroom and a cultivation method thereof. Background technique [0002] Palm-shaped rose ears (net-capped powder mushroom), the umbrella-shaped cap that curls inward on the edge of the mushroom body and the continuous mushroom stalk are its main features. It is edible and delicious. The stipe is crisp, sweet and tender, and the cap is rich in gum , high polysaccharide content, the stipe part will naturally secrete golden yellow sticky water droplets in the early stage of growth, and then gradually turn orange and dark red, the cap part will emit a special fruity smell when growing, and people with a normal sense of smell It can be smelled that the boiled cooked food tastes sweet after entering the mouth. It is suitable for soup, stir-fry and stew. Fresh mushrooms can also be used to make tea. The soup is pink or dark red and ha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G18/00A01G18/40A01G18/20
CPCA01G18/00A01G18/40A01G18/20
Inventor 图力古尔李中魁
Owner 河南果菇蓉生物科技有限公司
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