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Tissue culture technique of Seven Lucky Gods

A tissue culture and process technology, applied in the field of Qifushen tissue culture process, can solve the problems of growth cycle, survival rate limitation, unfavorable production, etc., and achieve the effect of moderate number of leaves, good growth and strong root system.

Active Publication Date: 2022-04-19
福建三杉生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no special tissue culture technology for the Seven Lucky Gods. In the experiment, it was found that although the tissue culture method of other succulent varieties can also be used to reproduce the Seven Lucky Gods, it is not conducive to production due to the limitation of the growth cycle and survival rate.

Method used

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  • Tissue culture technique of Seven Lucky Gods
  • Tissue culture technique of Seven Lucky Gods
  • Tissue culture technique of Seven Lucky Gods

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] The tissue culture technique of the Seven Lucky Gods comprises the following steps:

[0044]1) mother plant pretreatment: select the female parent whose color is light pink green, leaf ovate, thicker, blunt leaf end, disease-free, worm-free, stop watering 7 days before seed production, make the substrate dry, The whole potted plant is slightly wilted;

[0045] After drying, the 10mm-thick soil layer below the surface of the substrate is dried uniformly, and it becomes loose sand when pinched by hand, and it is not sticky;

[0046] 2) Explant treatment: cutting terminal buds from the female parent, and obtaining explants after disinfection;

[0047] Disinfection treatment is as follows: rinse the excised tissue in 0.5‰ washing powder solution for 10 minutes, then rinse with water for 3 times; then rinse with 1‰ HgCl 2 The solution was soaked and disinfected for 10 minutes, and then soaked and cleaned with sterile water 5 times, each time for 4 minutes; then the discolo...

Embodiment 2

[0062] The difference between embodiment 2 and embodiment 1 is that step 2) is different, and step 2 of embodiment 2) is:

[0063] 2) Explant treatment: cutting young and tender leaves from the female parent, and obtaining explants after disinfection;

[0064] Disinfection treatment is as follows: rinse the excised tissue in 0.5‰ washing powder solution for 5 minutes, then rinse twice with water; then rinse with 1‰ HgCl 2 The solution was soaked and disinfected for 8 minutes, and then soaked and washed with sterile water for 3 times, each time for 3 minutes; then the discolored part of the tissue section was excised.

Embodiment 3

[0066] The difference between embodiment 3 and embodiment 1 is that step 2) is different, and step 2 of embodiment 2) is:

[0067] 2) Explant treatment: cutting old leaves from the female parent, and obtaining explants after disinfection;

[0068] Disinfection treatment is as follows: rinse the excised tissue in 0.5‰ washing powder solution for 10 minutes, then rinse with water for 3 times; then rinse with 1‰ HgCl 2 The solution was soaked and disinfected for 9 minutes, and then soaked and washed with sterile water for 4 times, each time for 5 minutes; then the discolored part of the tissue section was excised.

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Abstract

The invention discloses a tissue culture process of Seven Lucky Gods, belonging to the technical field of plant tissue culture, comprising the following steps: mother plant pretreatment, explant treatment, primary culture, subculture, bacterial culture, proliferation culture and rooting culture. This tissue culture process is specially used for the cultivation and reproduction of the Seven Lucky Gods. Compared with the tissue culture process directly applied to other succulents, the Seven Lucky Gods with more buds can be obtained by changing the composition of the medium and optimizing the culture conditions. Rooted seedlings, and the obtained rooted seedlings grow well, grow strong, and can be directly used for rooting culture; and finally, the seedlings obtained through rooting culture have strong root systems, high plants, and moderate number of leaves, and are suitable for direct planting.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a tissue culture technique of the Seven Lucky Gods. Background technique [0002] Seven Lucky Gods (Echeveria secunda) is a succulent plant of the genus Echeveria in the sedum family, native to Mexico. Likes a warm, dry and sunny environment. Not cold-resistant, semi-shade and drought-tolerant, afraid of stagnant water and hot sun exposure, short stems, ovate leaves, thicker, light pink-green, white powder on the surface, round and blunt leaf ends. It is suitable for fertile, loose and well-drained sandy loam. The suitable temperature for growth is 18-25°C, and the temperature in winter is not lower than 5°C. Seven Lucky Gods, like most succulents of the genus Echeveria, can be cultivated by cuttings. But adopting this traditional method to cultivate, the breeding time is long and the reproduction coefficient is low. At present, there is no special t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 潘国余林萍
Owner 福建三杉生物科技有限公司
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