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A kind of Gynostemma pentaphyllum active ingredient dammarane type triterpene saponin and its separation and application

An active ingredient, dammarane-type technology, which is applied in the field of separation of Gynostemma pentaphyllum active parts and its main active ingredients, and can solve problems such as preparation and activity evaluation that have not been reported.

Active Publication Date: 2022-03-25
LANZHOU INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the studies on Gynostemma pentaphyllum are all Gynostemma pentaphyllum extract or total saponins of Gynostemma pentaphyllum, and there is no report on the preparation and activity evaluation of functional active parts in the extract

Method used

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  • A kind of Gynostemma pentaphyllum active ingredient dammarane type triterpene saponin and its separation and application
  • A kind of Gynostemma pentaphyllum active ingredient dammarane type triterpene saponin and its separation and application
  • A kind of Gynostemma pentaphyllum active ingredient dammarane type triterpene saponin and its separation and application

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Embodiment Construction

[0069] The extraction method and activity of the active part of Gynostemma pentaphyllum of the present invention will be further described below through specific examples.

[0070] Grind the dried aerial part of Gynostemma pentaphyllum, heat and reflux extract with 70% ethanol-water for 3 times, each time for 1-2 hours, combine the extracts, concentrate until there is no alcohol smell, and purify with D101 macroporous adsorption resin to obtain the active part JB2020. JB2020 was dissolved in water and extracted with petroleum ether (60-90°C), ethyl acetate and n-butanol respectively to obtain petroleum ether phase, ethyl acetate phase, n-butanol phase and water phase.

[0071] The ethyl acetate phase (200 g) was separated on a silica gel column, and chloroform-methanol (70:1~10:1, v / v) gradient elution was used to obtain 7 fractions (Fr. A~ Fr. G). Fr. D was separated by preparative liquid phase (methanol / water = 85 / 15, v / v was the mobile phase, the flow rate was 10mL / min; the...

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Abstract

The invention provides a dammarane-type triterpene saponin compound as an active component of Gynostemma pentaphyllum and a separation method thereof. Experiments show that the isolated 8 dammarane-type triterpenoid saponin compounds can significantly prolong the time of resistance to atmospheric airtight hypoxia and sodium nitrite hypoxia in mice, reduce the mortality of acute hypoxia, and have better anti-hypoxia resistance. Oxygen effect; it can significantly prolong the time that mice fall on the rotarod, prolong the time of mice swimming with weight, and has a good anti-fatigue effect; hypoxia can lead to cognitive dysfunction in mice, and Gynostemma pentaphyllum was found through water maze experiment The active site pretreatment will protect the learning and cognition ability of mice to a certain extent, so it can be used in the preparation of anti-fatigue, anti-oxygen hypoxia tolerance and improving learning and cognition drugs.

Description

technical field [0001] The invention relates to the separation of the active part of Gynostemma pentaphyllum and its main active components, in particular to a dammarane-type triterpene saponin and its separation method of the active component of Gynostemma pentaphyllum with functions of anti-fatigue, anti-hypoxia and improving learning and cognition, belonging to The field of plant active ingredient separation and the field of medicine and health care. Background technique [0002] Gynostemma ( Gynostemma pentaphyllum (Thunb.) Makino), is a herbaceous climbing plant of Cucurbitaceae and Gynostemma genus, the whole herb is used as medicine, and it is included in the "medicinal materials and decoction pieces not included in the prescription preparations" part of the 2015 edition of "Chinese Pharmacopoeia". Gynostemma pentaphyllum has the effects of delaying aging, anti-cancer, improving mental activity, improving brain function, enhancing human immunity, regulating endocrine...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07J71/00C07J73/00C07J17/00A61P39/00A61P25/28A61K36/424
CPCC07J71/0005C07J73/003C07J17/005A61P39/00A61P25/28A61K36/424
Inventor 杨军丽孟宪华王铖博
Owner LANZHOU INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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