Digestive tract tumor PDX model and construction method of standardized model library

Abstract

The invention provides a digestive tract tumor PDX model and a construction method of a standardized model library. The method comprises the following steps: conducting sampling, treating a collected peripheral blood sample of a digestive tract tumor, obtaining tumor cells, conducting treating and culturing, stably passing the tumor cells to n generations, constructing a digestive tract tumor PDX model and evaluating the model. A Chinese population human-derived digestive tract tumor transplantation animal model and a corresponding primary tumor cell line are established in a digestive tract tumor PDX standardized model library, an important in-vivo model is provided for biological research of tumors, searching of diagnostic markers and drug screening, randomness and blindness of drug use are avoided, disturbance by factors such as original tissue samples is avoided, the digestive tract tumor PDX model can be stably subcultured to three generations or more, and the construction of the digestive tract tumor PDX model standard sample library provides bioinformatics for exploring new targets and searching and analyzing clinical characteristics of digestive tract tumors, and assists in research of a fatal mechanism of digestive tract tumors and treatment of patient tumors.

Description

Technical field [0001] The present invention relates to the field of biological and medical models and model libraries, in particular, involving a construction method of a digestive tract tumor PDX model and a standardized model library. Background technique [0002] According to statistics, my country's gastrointestinal tumors account for 43.3% of all cancer morbidity, almost occupying half-Wanjiang Mountain, is a number of killers in the tumor industry. Human tissue xenograft model, also known as the PDX model, is a tumor tissue of tumor patients into a severe immunodeficiency mouse (NSG) body, and the tumor tissue is constructed in mice. Tumor model, auxiliary tumor treatment. The PDX model retains the basic characteristics of the primary tumor microenvironment and cells, forming the first generation of transplanted tumors, to be grown to a certain size to take the transplanted tissue, and then transplanted into a new batch of mice, forming the second Sending tumor, so repeate...

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Problems solved by technology

This model retains the microenvironment of the parental tumor growth as much as possible, such as infiltrated lymphocytes, extracellular matrix, microvessels, etc. PDX model transplantation methods include subcutaneous transplantation, renal subcapsular xenografting, and orthotopic transplantation. Compared with CDX models, they can maintain the molecular, genetic, and pathological characteristics of the primary tumor. There are few studies on the model. Since the PDX model has differentiated characteristics, there is no uniform standard. The use of immunodeficiency mice (NSG) for transplantation and passage to construct PDX models is easily disturbed by the factors of the original tissue samples, and the process standardization is poor. The success rate of model construction, and there is no standard sample library of PDX model of gastrointestinal tumors, which cannot provide bioinformatics for exploring new targets and finding and analyzing clinical characteristics of gastrointestinal tumors

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