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Polypeptides and methods for improving skin conditions

A technology for skin and pathological conditions, applied in the field of peptides and methods for improving skin pathological conditions, which can solve problems such as reducing the quality of life

Pending Publication Date: 2021-07-23
英特肽治疗有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Such wounds place a huge financial burden on the healthcare system while greatly reducing the quality of life of those affected

Method used

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  • Polypeptides and methods for improving skin conditions
  • Polypeptides and methods for improving skin conditions
  • Polypeptides and methods for improving skin conditions

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0197] Example 1: Polypeptides RSKAKNPLYRRRRRRRR and rrrrrrrrylpnkaksr promote wound healing.

[0198] figure 1 and figure 2 The polypeptide IK34720 (SEQ ID NO:3-RSKAKNPLYRRRRRRRR) ( figure 1 ) and dextro-reversal sequence IK236770 (SEQ ID NO:4–rrrrrrrrrylpnkaksr) ( figure 2 ) on wound healing over 10 days in a murine wound model according to the following protocol.

[0199] In this study, three groups of 8 male ICR mice with an average weight of 22 g (± 2 g) were used. Animals were housed in individual cages during the study. Under isoflurane gas anesthesia, the shoulders and back of each animal were shaved and induced on day 1 by removing the skin, including panniculus carnosus and adhesions, using a sharp punch (12 mm inner diameter). Wound.

[0200] After wound induction, polypeptides IK34720 and IK236770 were topically applied to the wound site at a dose of 10 μg per animal. Additionally, the positive control group was administered 10 μg per animal of an α2A agon...

Embodiment 2

[0206] Example 2: The polypeptide RSKAKNPLYRRRRRRRR inhibits pCREB formation in primary keratinocytes and restores UV-induced inhibition of the TGFβ receptor (TGFβRII) when applied after exposure of keratinocytes to sun-mimicking UV radiation.

[0207] CREB is phosphorylated at serine at position 133 (Ser-133) in response to exposure of cells to UV irradiation. Increased phosphorylation of CREB is associated with a range of functions including cell proliferation, cell cycle, metabolism, DNA repair, differentiation, inflammation, angiogenesis, immune response and cell survival. Thus, aberrant CREB phosphorylation is associated with tumor development, malignancy and survival (Steven. A and Seliger B., Oncotarget. 2016 Jun 7;7(23):35454–35465).

[0208] The effect of polypeptide IK34720 (SEQ ID NO:3) on CREB phosphorylation was assessed using the following protocol.

[0209] Keratinocytes were harvested and cultured as previously described (Gupta et al., J Invest Dermatol, 2007,...

Embodiment 3

[0220] Example 3: The polypeptide RSKAKNPLYRRRRRRRR increases ATP levels in primary keratinocytes exposed to solar simulated UV irradiation.

[0221] Figure 4 The effect of polypeptide IK34720 (SEQ ID NO:3) on ATP levels in primary keratinocytes exposed to solar simulated UV irradiation is illustrated. Inadequately repaired DNA damage after UV exposure has been shown to be a causative factor in the development of skin cancer. DNA repair requires energy, but after UV exposure, skin cells have a limited ability to produce ATP, the main source of energy.

[0222] The following protocol was used to assess the effect of polypeptide IK34720 (SEQ ID NO:3) on intracellular ATP after UV exposure.

[0223] Primary keratinocytes were harvested as described above in Example 2 and were treated in the presence or absence of calcitriol (positive control) or polypeptide IK34720 (SEQ ID NO:3; RSKAKNPLYRRRRRRRR) at concentrations described in Example 2 They were exposed to ssUV irradiation ...

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Abstract

The present invention relates to polypeptides, compositions and methods for preventing and / or treating skin conditions including dermal aging and skin conditions associated with UV exposure.

Description

technical field [0001] The present disclosure relates to polypeptides, compositions and methods for preventing and / or treating skin conditions including skin aging and skin conditions associated with UV exposure. Background technique [0002] The cellular and molecular mechanisms of skin aging, skin injury, and cutaneous wound healing are not fully understood. [0003] Skin aging is associated with loss of elasticity, dryness, wrinkles and hyperpigmentation. Ultraviolet (UV) irradiation of the skin, and particularly exposure to UVB with a wavelength of 290 nm to 320 nm, is known to cause chronic skin damage, including premature aging of the skin and induction of skin cancer. Skin cancer costs the Australian health system more than $700 million annually, and an estimated over 138,000 new cases of skin cancer were diagnosed in 2018. [0004] UVB irradiation has been shown to induce the expression of cyclooxygenase 2 (COX-2), where upregulation of COX-2 plays a functional rol...

Claims

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Application Information

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IPC IPC(8): C07K7/08A61P17/02A61P17/18A61K9/00A61K38/00A61P17/16A61Q17/04A61K8/64
CPCC07K7/08A61P17/02A61P17/16A61P17/18A61Q17/04A61K8/64A61K9/0014A61P17/00A61Q19/08C07K7/06C07K2319/10A61K38/00A61Q19/00A61Q19/004
Inventor M·阿格雷斯
Owner 英特肽治疗有限公司
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