Method for screening volatile matter for repelling macrosiphum avenae from chrysanthemum filial generation population
A long tube aphid and volatile technology, applied in botany equipment and methods, pest repellants, gardening methods, etc., can solve the problems of abnormal growth and development of chrysanthemums, chemical control is not an environmentally friendly way, wilting, etc. The effect of sample size
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[0046] This embodiment provides a method for screening the volatiles from the hybrid offspring of Hibiscus chrysanthemum and chamomile for repelling Aphis chrysanthemum, the steps are as follows:
[0047] 1. Obtaining clones of hybrid progeny: select the foot buds of hibiscus chrysanthemum and chamomile hybrid progeny in good growth state and without pests and diseases, cut 6-8 cm long cuttings, and cut them into peat: perlite: vermiculite = 2:1:1 in the matrix. 5 strains were multiplied for each strain.
[0048] 2. Determination of the resistance of the hybrid progeny to A. chrysanthemum: when the clones of the hybrid progeny in step 1 grow to 8-12 leaves, artificially inoculate the wingless adults within 24 hours after the molt of the 4th instar nymph of A. chrysanthemum. After the inoculation was completed, the plants were covered with a PC box with air holes of 7×7×12 cm, and the bottom substrate was covered with a filter paper with a diameter of 10 cm. On the 10th day a...
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