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Microbial agent

A microbial agent and seed technology, applied in the field of microbial agent, can solve problems such as single function and non-availability, and achieve the effects of improving the soil environment, enhancing the absorption capacity of roots, and reducing the content of heavy metals

Pending Publication Date: 2021-07-30
天津益农生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, kasugamycin, polyoxin, Jinggangmycin, agromycin, streptomycin, etc. are used as fungicides; abamectin, acaricide, etc. are used as insecticides; bialaphos is used as herbicides; Plant growth regulators include gibberellin, etc., but the existing microbial agents do not have the functions of improving soil, protecting plants and promoting plant growth at the same time.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A preparation method of microbial inoculum, comprising the following steps:

[0024] S1, after solid-state activation culture of Bacillus lateralis, Brevibacillus soil and Bacillus amyloliquefaciens respectively, three kinds of bacterial suspensions with concentrations of 2×10^8 CFU / ml were obtained;

[0025] S2, the preparation requires a primary seed medium, a secondary seed medium and a fermentation medium;

[0026] S3, inoculating the three kinds of bacterial suspensions to the primary seed culture medium with an inoculation amount of 3% by volume percentage, and culturing to obtain three primary seed bacterial suspensions with a concentration of 2×10^10 CFU / ml;

[0027] S4, three kinds of primary seed bacteria suspensions are mixed with equal volumes, then transfer to the secondary seed culture medium with an inoculum size of 1% by volume percentage, and the secondary seed culture medium with a mixed bacterial concentration of 1×10^11 CFU / ml is cultivated. Grade s...

Embodiment 2

[0031] A preparation method of microbial inoculum, comprising the following steps:

[0032] S1, after solid-state activation culture of Bacillus lateralis, Brevibacillus soil and Bacillus amyloliquefaciens respectively, three kinds of bacterial suspensions with concentrations of 2×10^8 CFU / ml were obtained;

[0033] S2, the preparation requires a primary seed medium, a secondary seed medium and a fermentation medium;

[0034] S3. Inoculate the three kinds of bacterial suspensions into the primary seed culture medium with an inoculation amount of 4% by volume percentage, and cultivate three kinds of primary seed bacterial suspensions with a concentration of 3×10^10 CFU / ml;

[0035] S4, three kinds of primary seed bacteria suspensions are mixed with equal volumes, then transfer to the secondary seed culture medium with an inoculum size of 2% by volume percentage, and the secondary seed culture medium with a mixed bacterial concentration of 2×10^11 CFU / ml is cultivated. Grade se...

Embodiment 3

[0039] A preparation method of microbial inoculum, comprising the following steps:

[0040] S1, after solid-state activation culture of Bacillus lateralis, Brevibacillus agroides and Bacillus amyloliquefaciens bacterial strains respectively, three kinds of bacterial suspensions with concentrations of 3×10^8 CFU / ml were obtained;

[0041] S2, the preparation requires a primary seed medium, a secondary seed medium and a fermentation medium;

[0042] S3. Inoculate the three kinds of bacterial suspensions into the primary seed culture medium with an inoculation amount of 7% by volume percentage, and cultivate three kinds of primary seed bacterial suspensions with a concentration of 4×10^10 CFU / ml;

[0043] S4, the three kinds of primary seed bacteria suspensions are mixed with equal volumes, then transferred to the secondary seed culture medium with an inoculum size of 3% by volume percentage, and the secondary seed culture medium that is cultivated to obtain a mixed bacterial con...

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PUM

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Abstract

The invention relates to the field of microbial agents, in particular to a microbial agent which can effectively improve the soil environment and prevent and improve the symptoms of root rot, root rot, seedling death, damping-off, damping-off, water chestnut blight and root retting of melons and fruits, vegetables, fruit trees, flowers, cotton and rice crops.

Description

technical field [0001] The invention relates to the field of microbial bacterial agents, in particular to a microbial bacterial agent. Background technique [0002] The production of microorganisms is used in agricultural planting to be widely promoted, and the commercialized agricultural antibiotics are used in almost all fields of pesticides. Among them, kasugamycin, polyoxin, Jinggangmycin, agromycin, streptomycin, etc. are used as fungicides; abamectin, acaricide, etc. are used as insecticides; bialaphos is used as herbicides; Plant growth regulators include gibberellin, etc., but the existing microbial agents do not have the functions of improving soil, protecting plants and promoting plant growth at the same time. Contents of the invention [0003] The purpose of the present invention is to solve the shortcoming that exists in the background technology, and propose a kind of microbial bacterial agent. [0004] In order to achieve the above object, the technical sol...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01B79/00A01G13/00A01G7/06C12R1/07C12R1/08
CPCC12N1/20A01B79/00A01G13/00A01G7/06
Inventor 位全龙
Owner 天津益农生物科技有限公司
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