PCR primer and kit for rapidly identifying asarum plant material, and applications of PCR primer

Abstract

The invention discloses a PCR primer and a kit for rapidly identifying asarum plants, and applications of the PCR primer. The nucleotide sequence of the primer is as follows: Asa_F: 5'-TATCTCTTTCTTTTTTTCAAAAGG-3', and Asa_R: 5'-ATGGGCTTTTGCCAATGAGCCAATC-3'. The invention further discloses a method and a kit for rapidly identifying the asarum plants. The method and the kit are strong in specificity, high in sensitivity and high in accuracy.

Description

technical field [0001] The invention relates to the technical field of molecular biology, in particular to a PCR primer for rapid identification of Asarum plants, a kit and an application thereof. Background technique [0002] Aristolochiaceae (Aristolochiaceae) belongs to the angiosperm phylum dicotyledon class Magnolia subclass Aristolochiales (Aristolochiales), divided into Aristolochia and Asarinae, including more than 600 species of plants, and most of them contain Aristolochia acid. The main medicinal materials include Aristolochia, Akebia officinalis, Fangji, Asarum, Qingmuxiang, Tianxianteng, Cinnabar lotus, Horseshoe incense, etc. Aristolochia plants mainly contain aristolochone, greenwood acid, quaternary ammonium alkaloids, magnolanine, cylindrine and other components. The main components of most species of Asarum (scientific name: Asarum L.) are methyl eugenol, safrole, asarone, agaric acid, asarum ketone and alkaloids with cardiotonic effect. [0003] Aristol...

AI Technical Summary

Problems solved by technology

Based on the following reasons, it is necessary to develop a rapid identification technology for Asarum: (1) The root of the medicinal part of Asarum is easily confused with Bai Wei, Xu Changqing and other medicinal materials, and the phenomenon of confusion and adulteration of Chinese herbal medicines occurs frequently. It is difficult to identify by conventional morphological methods; (2) Asarum is used as a Chinese herbal medicine in different parts, and the products on the market cannot be displayed as a whole whole plant, and cannot pass morphological identification; (3) As people invest more in diet and health care, , especially the variety of dishes pursued in traditional Chinese medicine diet therapy is also changing with each passing day. At the same time, pure natural and pollution-free wild vegetables are sought after by the public. Aristolochic acid poisoning is caused by eating medicated diets and Chinese herbal medicine pills containing aristolochic acid However, due to the lack of effective rapid identification technology, the cause of poisoning cannot be diagnosed in time, and even misdiagnosis occurs.

[0004] To sum up, food safety is related to the national economy and the people's livelihood, and the species identification technology of food materials is an important link in the food safety chain. Foods with unknown raw material sources or composition composition will bring huge risks and hidden dangers to food safety. Conventional morphological identification methods can solve the problem. These problems cannot be solved, so it is necessary to develop an accurate and rapid technology for identifying the ingredients of Asarum in food

In the past ten years, a variety of molecular biology techniques have been applied to the rapid identification of other species, such as: DNA barcoding (DNA Barcoding), PCR technology, restriction fragment length polymorphism (Restriction Fragment Length Polymorphism, RFLP), random amplification Polymorphic DNA technology (Random Amplified PolymorphicDNA, RAPD), amplified fragment length polymorphism technology (Amplified Fragment Length Polymorphism, AFLP) and gene chip technology, etc. The rapid identification method of plant species in the genus Simulacrum fails to fully meet the detection and inspection requirements of my country's market supervision and food safety supervision

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