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Method for detecting prolactin monomer in serum

A detection method and technology of prolactin, applied in the direction of measuring devices, biological tests, material inspection products, etc., can solve the problems of lack of detection methods for prolactin monomers in serum

Pending Publication Date: 2021-07-30
AFFILIATED HUSN HOSPITAL OF FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The purpose of the present invention is to provide a method for detecting prolactin monomers in serum, thereby solving the problem of lack of detection methods for prolactin monomers in serum in the prior art

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  • Method for detecting prolactin monomer in serum

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Embodiment 1

[0019] Example 1 Construction of Prolactin Monomer Detection Method

[0020] Option One

[0021] Provided is a method for detecting prolactin monomers in serum, comprising the following steps:

[0022] 1) Add 200 μL of serum to an equal volume of 20% polyethylene glycol 6000 solution for pretreatment and precipitation, wherein the 20% polyethylene glycol 6000 solution is prepared by dissolving 20 g of polyethylene glycol 6000 in 1000 ml of deionized water have to;

[0023] 2) Vortex mixing for 5 minutes at room temperature;

[0024] 3) Centrifuge at 13400rpm for 10 minutes;

[0025] 4) Take the supernatant, and use Roche Cobas 8000 electrochemiluminescence immunoassay analyzer to detect the concentration of prolactin monomer therein;

[0026] 5) The detection result is multiplied by the dilution factor 2 to obtain the prolactin monomer concentration level in the serum.

[0027] Option II

[0028] Provided is a method for detecting prolactin monomers in serum, comprising ...

Embodiment 2

[0064] Example 2 Establishing reference intervals for prolactin monomers in both sexes in this region

[0065] This example also establishes for the first time the prolactin monomer reference interval of the healthy sex population after polyethylene glycol precipitation in this region, and the establishment of the prolactin monomer reference interval is limited to the Roche electrochemiluminescence system. A total of 240 serum samples of healthy subjects were collected, aged from 18 to 60 years old, and the serum samples of women who used estrogen-containing contraceptives or received hormone therapy were excluded. There is a significant difference between men and women in the test results of prolactin monomers in the population, and the distribution is not normal, so the reference interval adopts the two-sided 2.5-97.5 percentile, women: 3.4-18.5ng / mL; men: 2.7-13.1ng / mL mL. Due to the innovation of the evaluation criteria, the reference interval of prolactin monomer is adop...

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Abstract

The invention provides a method for detecting prolactin monomer in serum, which comprises the following steps: S1, taking a proper amount of serum, adding the serum into an isometric 25% polyethylene glycol 6000 solution, and carrying out pretreatment precipitation; s2, carrying out vortex mixing at room temperature for 5-10 minutes; s3, performing high-speed centrifugation for 10-15 minutes, and taking supernatant; and S4, detecting the supernatant obtained in the step S3 by using an immunoassay instrument, and multiplying the obtained detection result by a dilution factor 2 to obtain the concentration of the prolactin monomer in the serum. According to the present invention, the detection of the prolactin monomer in the serum is achieved for the first time, the basis is provided for the diagnosis of the hyperprolactinemia, the diagnosis and treatment time is saved for the patient, and the diagnosis and treatment cost is reduced, such that the detection method provided by the present invention has the promotion value in the biological molecule clinical detection.

Description

technical field [0001] The invention relates to the technical field of biomolecular detection, in particular to a method for detecting prolactin monomers in serum. Background technique [0002] Prolactin, also known as prolactin, is one of the hormones secreted by the pituitary gland. During late pregnancy and lactation, women secrete prolactin to promote mammary gland development and lactation. The highest level of prolactin in the serum of non-pregnant women generally does not exceed 20 ng / ml. [0003] Prolactin is secreted by specialized cells concentrated on both sides of the posterior pituitary gland, and originates from the same cell as growth hormone. Elevated prolactin, medically known as hyperprolactinemia, is a hypothalamic-pituitary-gonadal axis dysfunction disease, the main symptoms of which are significantly reduced menstrual flow, thin menstruation or even amenorrhea, infertility, and galactorrhea , menopausal symptoms, etc. [0004] Excessive prolactin wil...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/74G01N1/28G01N1/34
CPCG01N33/74G01N1/28G01N1/34G01N2333/5752
Inventor 胡尧张心菊关明
Owner AFFILIATED HUSN HOSPITAL OF FUDAN UNIV